Reviewed, UniProtKB/Swiss-Prot Q7VHN8 (SYE1_HELHP)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	HH_0926

Organism

Helicobacter hepaticus [Complete proteome] [HAMAP]

Taxonomic identifier

32025 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity. HAMAP MF_00022
Subcellular location	Cytoplasm HAMAP MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000119574
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022
	Motif	238 – 242	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	241	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VHN8-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 1230249C82263C3D	FASTA	464	52,985
	10 20 30 40 50 60 MDKIVTRFAP SPTGYLHIGG LRTALFNYLY ARANGGKFLL RIEDTDLARN SMEATKAIIE 70 80 90 100 110 120 SFEWAGLDYD GEVVYQSQRF DLYKTYIQQL LESKKAYYCY MSKEELDALR KEQEKNKQTP 130 140 150 160 170 180 RYDNRYRDFT GIPPQGIQPV VRIKAPLEGN IEFEDGIKGQ ISINAKEIDD FIIARSDGTP 190 200 210 220 230 240 TYNFVVAVDD ALMGITDVIR GDDHLSNTPK QIIIYNALGF ALPRFFHVPM ILNSQGKKLS 250 260 270 280 290 300 KRDGAMGVMD YAKMGYLPEA ILNFLVRLGW SYGDKEIFSL EEMLELFNPN ELNSSPSAYN 310 320 330 340 350 360 EDKLLWLNQH YIKYMDNTLL EQLLGHFGVT KLSESKREIL YPALKDRSNT LVSFAQGFNE 370 380 390 400 410 420 VMNAPCSYDE KMRIKLDANA TQWLNELCKN LNMASWEDNP NIIESYLHQF ALEHQIKIGK 430 440 450 460 LMPTLRCALL GKSGGIGVCE ALAVLGIEES MRRIYTFIGY QNNI

References

[1]

"The complete genome sequence of the carcinogenic bacterium Helicobacter hepaticus." Suerbaum S., Josenhans C., Sterzenbach T., Drescher B., Brandt P., Bell M., Droege M., Fartmann B., Fischer H.-P., Ge Z., Hoerster A., Holland R., Klein K., Koenig J., Macko L., Mendz G.L., Nyakatura G., Schauer D.B. , Shen Z., Weber J., Frosch M., Fox J.G. Proc. Natl. Acad. Sci. U.S.A. 100:7901-7906(2003) [PubMed: 12810954] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51449 / 3B1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017125 Genomic DNA. Translation: AAP77523.1.
RefSeq	NP_860457.1.
3D structure databases
SMR	Q7VHN8. Positions 4-459.
ModBase	Search...
Genome annotation databases
GeneID	1492615.
GenomeReviews	Gene locus HH_0926 in contig AE017125_GR.
KEGG	hhe:HH0926.
NMPDR	fig|235279.1.peg.926.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	DLVIQRS.
Enzyme and pathway databases
BioCyc	HHEP235279:HH_0926-MONOMER.
BRENDA	6.1.1.17. 279780.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_HELHP

Accession

Primary (citable) accession number: Q7VHN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)