Q7VHF9 (NADE_HELHP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	HH_1008

Organism

Helicobacter hepaticus (strain ATCC 51449 / 3B1) [Complete proteome] [HAMAP]

Taxonomic identifier

235279 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152172
Regions
	Nucleotide binding	27 – 34	8	ATP By similarity
Sites
	Active site	29	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VHF9 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 1C91A0144716695E	FASTA	274	30,669
	10 20 30 40 50 60 MKHFVNPCIH FIQKQLQERG FKKVVLGLSG GIDSAVVATL ATLALGSENV RALLMPSLSS 70 80 90 100 110 120 NEEHFNDAFN LAHNLELESK IIQLAPFQEN FAKQEGMDLS GKYMEKLDMN QKMRMGNFCA 130 140 150 160 170 180 RIRMTMLYDC ASADNALVLG TSNKSEILLG YGTIFGDLAY AINPIGGLYK TQIFAFARAL 190 200 210 220 230 240 NVPQEIIAKK PSADLFANQS DETDLGYNYA DIDTFLEAFE KLGGVEATQN KEREHIKEKL 250 260 270 KNAGFECNMI ESLSTRVWNN TFKRTKPTIL EYKV

References

[1]

"The complete genome sequence of the carcinogenic bacterium Helicobacter hepaticus." Suerbaum S., Josenhans C., Sterzenbach T., Drescher B., Brandt P., Bell M., Droege M., Fartmann B., Fischer H.-P., Ge Z., Hoerster A., Holland R., Klein K., Koenig J., Macko L., Mendz G.L., Nyakatura G., Schauer D.B. , Shen Z., Weber J., Frosch M., Fox J.G. Proc. Natl. Acad. Sci. U.S.A. 100:7901-7906(2003) [PubMed: 12810954] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51449 / 3B1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017125 Genomic DNA. Translation: AAP77605.1.
RefSeq	NP_860539.1. NC_004917.1.
3D structure databases
ProteinModelPortal	Q7VHF9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1492697.
GenomeReviews	Gene locus HH_1008 in contig AE017125_GR.
KEGG	hhe:HH1008.
NMPDR	fig|235279.1.peg.1008.
PATRIC	20589488. VBIHelHep90276_1006.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	VNIGPAV.
Enzyme and pathway databases
BioCyc	HHEP235279:HH_1008-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_HELHP

Accession

Primary (citable) accession number: Q7VHF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program