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Q7VGX8

- SYI_HELHP

UniProt

Q7VGX8 - SYI_HELHP

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Helicobacter hepaticus (strain ATCC 51449 / 3B1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei573 – 5731Aminoacyl-adenylateUniRule annotation
    Binding sitei617 – 6171ATPUniRule annotation
    Metal bindingi902 – 9021ZincUniRule annotation
    Metal bindingi905 – 9051ZincUniRule annotation
    Metal bindingi918 – 9181ZincUniRule annotation
    Metal bindingi921 – 9211ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciHHEP235279:GHUA-1226-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:HH_1190
    OrganismiHelicobacter hepaticus (strain ATCC 51449 / 3B1)
    Taxonomic identifieri235279 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    ProteomesiUP000002495: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 930930Isoleucine--tRNA ligasePRO_0000098397Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi235279.HH1190.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7VGX8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi614 – 6185"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7VGX8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYKETLILP DTSFPMRGNL PANEPHTYAK WRDEKLYSKL KNAHSGAKNF    50
    CLHDGPPYAN GHLHIGHALN KILKDIIVKY HYFKGENIRY TPGWDCHGLP 100
    IEQQVEQKIG KAKKDNLNKT KLRELCRNHA QKFIQIQSDE FQALGVLGDF 150
    ENPYKTMDFA FEAQIYRSLI ELVKEGLLAE RSKPIYWSWA CETALADAEV 200
    EYQDKQSDSI FVAFTLSDSA LQSLGISQGK VIIWTTTPWT LPANVGIALN 250
    PSESYVLTDK GYIVAKALLE KVCENIDIGI SKREFKAQEF ERLEAINPLN 300
    GRTSLIVLGE HVSINDGTGA VHTAPGHGED DYYIGLKYGL EVIMPVDDRG 350
    NFSPLIESMG LVPKEFANEF VGKNIFDTHE SIFKILGKAL LKQDVITHSY 400
    PHCWRSHKPV IYRATAQWFI LLDKPFYQGK TLKELALEEL DKVRFYPQNG 450
    KNRIYSMIEN RPDWCISRQR DWGVPIAFLI DKITNVALLD EAVLEHIACI 500
    FEKEGCDAWW SYEIKDLLPP SHKHLAENLT KSKHILDVWF DSGSTWSAVL 550
    ENEYKGAGNN YDAGSHPADM YLEGSDQHRG WFQSSLLLSC AIAKRAPYKQ 600
    ILTHGFTFDR NGEKMSKSKG NVISPSEIIK TQGSEILRLW VALSHYQSDQ 650
    NISDEILKQV GEQYRKIRNT IRFLLANASQ NSQDMVEKDE LDVIDRWILS 700
    VCDRVFDEAY EYFDEYEFAK AFQVVMSFLI NELSGIYLDL CKDILYCDDV 750
    SSLRRRAIQS ALVMILRRVL HFIAPVLTYT ADEAFRHSSA ALKGEYESIF 800
    DLPKLSCAQG YNLEINEDFT RLLRVREKFT ESLDNLKKQK IVKSSLEVLL 850
    QSPQEKEFAL LDRWLIVSGV CGKECKDKEE LARFMLEDKE EFVIYRAQNG 900
    RCERCWQFIV EESEGKLCKR CTSVIAQTKK 930
    Length:930
    Mass (Da):106,646
    Last modified:October 1, 2003 - v1
    Checksum:iFBBDA80B50BE692E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017125 Genomic DNA. Translation: AAP77787.1.
    RefSeqiNP_860721.1. NC_004917.1.
    WP_011116030.1. NC_004917.1.

    Genome annotation databases

    EnsemblBacteriaiAAP77787; AAP77787; HH_1190.
    GeneIDi1492878.
    KEGGihhe:HH1190.
    PATRICi20589834. VBIHelHep90276_1178.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017125 Genomic DNA. Translation: AAP77787.1 .
    RefSeqi NP_860721.1. NC_004917.1.
    WP_011116030.1. NC_004917.1.

    3D structure databases

    ProteinModelPortali Q7VGX8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 235279.HH1190.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP77787 ; AAP77787 ; HH_1190 .
    GeneIDi 1492878.
    KEGGi hhe:HH1190.
    PATRICi 20589834. VBIHelHep90276_1178.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci HHEP235279:GHUA-1226-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51449 / 3B1.

    Entry informationi

    Entry nameiSYI_HELHP
    AccessioniPrimary (citable) accession number: Q7VGX8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3