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Q7VGX8

- SYI_HELHP

UniProt

Q7VGX8 - SYI_HELHP

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Protein
Isoleucine--tRNA ligase
Gene
ileS, HH_1190
Organism
Helicobacter hepaticus (strain ATCC 51449 / 3B1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei573 – 5731Aminoacyl-adenylate By similarity
Binding sitei617 – 6171ATP By similarity
Metal bindingi902 – 9021Zinc By similarity
Metal bindingi905 – 9051Zinc By similarity
Metal bindingi918 – 9181Zinc By similarity
Metal bindingi921 – 9211Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHHEP235279:GHUA-1226-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:HH_1190
OrganismiHelicobacter hepaticus (strain ATCC 51449 / 3B1)
Taxonomic identifieri235279 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000002495: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 930930Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098397Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi235279.HH1190.

Structurei

3D structure databases

ProteinModelPortaliQ7VGX8.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi614 – 6185"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7VGX8-1 [UniParc]FASTAAdd to Basket

« Hide

MDYKETLILP DTSFPMRGNL PANEPHTYAK WRDEKLYSKL KNAHSGAKNF    50
CLHDGPPYAN GHLHIGHALN KILKDIIVKY HYFKGENIRY TPGWDCHGLP 100
IEQQVEQKIG KAKKDNLNKT KLRELCRNHA QKFIQIQSDE FQALGVLGDF 150
ENPYKTMDFA FEAQIYRSLI ELVKEGLLAE RSKPIYWSWA CETALADAEV 200
EYQDKQSDSI FVAFTLSDSA LQSLGISQGK VIIWTTTPWT LPANVGIALN 250
PSESYVLTDK GYIVAKALLE KVCENIDIGI SKREFKAQEF ERLEAINPLN 300
GRTSLIVLGE HVSINDGTGA VHTAPGHGED DYYIGLKYGL EVIMPVDDRG 350
NFSPLIESMG LVPKEFANEF VGKNIFDTHE SIFKILGKAL LKQDVITHSY 400
PHCWRSHKPV IYRATAQWFI LLDKPFYQGK TLKELALEEL DKVRFYPQNG 450
KNRIYSMIEN RPDWCISRQR DWGVPIAFLI DKITNVALLD EAVLEHIACI 500
FEKEGCDAWW SYEIKDLLPP SHKHLAENLT KSKHILDVWF DSGSTWSAVL 550
ENEYKGAGNN YDAGSHPADM YLEGSDQHRG WFQSSLLLSC AIAKRAPYKQ 600
ILTHGFTFDR NGEKMSKSKG NVISPSEIIK TQGSEILRLW VALSHYQSDQ 650
NISDEILKQV GEQYRKIRNT IRFLLANASQ NSQDMVEKDE LDVIDRWILS 700
VCDRVFDEAY EYFDEYEFAK AFQVVMSFLI NELSGIYLDL CKDILYCDDV 750
SSLRRRAIQS ALVMILRRVL HFIAPVLTYT ADEAFRHSSA ALKGEYESIF 800
DLPKLSCAQG YNLEINEDFT RLLRVREKFT ESLDNLKKQK IVKSSLEVLL 850
QSPQEKEFAL LDRWLIVSGV CGKECKDKEE LARFMLEDKE EFVIYRAQNG 900
RCERCWQFIV EESEGKLCKR CTSVIAQTKK 930
Length:930
Mass (Da):106,646
Last modified:October 1, 2003 - v1
Checksum:iFBBDA80B50BE692E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017125 Genomic DNA. Translation: AAP77787.1.
RefSeqiNP_860721.1. NC_004917.1.
WP_011116030.1. NC_004917.1.

Genome annotation databases

EnsemblBacteriaiAAP77787; AAP77787; HH_1190.
GeneIDi1492878.
KEGGihhe:HH1190.
PATRICi20589834. VBIHelHep90276_1178.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017125 Genomic DNA. Translation: AAP77787.1 .
RefSeqi NP_860721.1. NC_004917.1.
WP_011116030.1. NC_004917.1.

3D structure databases

ProteinModelPortali Q7VGX8.
ModBasei Search...

Protein-protein interaction databases

STRINGi 235279.HH1190.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAP77787 ; AAP77787 ; HH_1190 .
GeneIDi 1492878.
KEGGi hhe:HH1190.
PATRICi 20589834. VBIHelHep90276_1178.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci HHEP235279:GHUA-1226-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51449 / 3B1.

Entry informationi

Entry nameiSYI_HELHP
AccessioniPrimary (citable) accession number: Q7VGX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2003
Last modified: September 3, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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