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Q7VGX8

- SYI_HELHP

UniProt

Q7VGX8 - SYI_HELHP

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Helicobacter hepaticus (strain ATCC 51449 / 3B1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei573 – 5731Aminoacyl-adenylateUniRule annotation
Binding sitei617 – 6171ATPUniRule annotation
Metal bindingi902 – 9021ZincUniRule annotation
Metal bindingi905 – 9051ZincUniRule annotation
Metal bindingi918 – 9181ZincUniRule annotation
Metal bindingi921 – 9211ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciHHEP235279:GHUA-1226-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:HH_1190
OrganismiHelicobacter hepaticus (strain ATCC 51449 / 3B1)
Taxonomic identifieri235279 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
ProteomesiUP000002495: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 930930Isoleucine--tRNA ligasePRO_0000098397Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi235279.HH1190.

Structurei

3D structure databases

ProteinModelPortaliQ7VGX8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi614 – 6185"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7VGX8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYKETLILP DTSFPMRGNL PANEPHTYAK WRDEKLYSKL KNAHSGAKNF
60 70 80 90 100
CLHDGPPYAN GHLHIGHALN KILKDIIVKY HYFKGENIRY TPGWDCHGLP
110 120 130 140 150
IEQQVEQKIG KAKKDNLNKT KLRELCRNHA QKFIQIQSDE FQALGVLGDF
160 170 180 190 200
ENPYKTMDFA FEAQIYRSLI ELVKEGLLAE RSKPIYWSWA CETALADAEV
210 220 230 240 250
EYQDKQSDSI FVAFTLSDSA LQSLGISQGK VIIWTTTPWT LPANVGIALN
260 270 280 290 300
PSESYVLTDK GYIVAKALLE KVCENIDIGI SKREFKAQEF ERLEAINPLN
310 320 330 340 350
GRTSLIVLGE HVSINDGTGA VHTAPGHGED DYYIGLKYGL EVIMPVDDRG
360 370 380 390 400
NFSPLIESMG LVPKEFANEF VGKNIFDTHE SIFKILGKAL LKQDVITHSY
410 420 430 440 450
PHCWRSHKPV IYRATAQWFI LLDKPFYQGK TLKELALEEL DKVRFYPQNG
460 470 480 490 500
KNRIYSMIEN RPDWCISRQR DWGVPIAFLI DKITNVALLD EAVLEHIACI
510 520 530 540 550
FEKEGCDAWW SYEIKDLLPP SHKHLAENLT KSKHILDVWF DSGSTWSAVL
560 570 580 590 600
ENEYKGAGNN YDAGSHPADM YLEGSDQHRG WFQSSLLLSC AIAKRAPYKQ
610 620 630 640 650
ILTHGFTFDR NGEKMSKSKG NVISPSEIIK TQGSEILRLW VALSHYQSDQ
660 670 680 690 700
NISDEILKQV GEQYRKIRNT IRFLLANASQ NSQDMVEKDE LDVIDRWILS
710 720 730 740 750
VCDRVFDEAY EYFDEYEFAK AFQVVMSFLI NELSGIYLDL CKDILYCDDV
760 770 780 790 800
SSLRRRAIQS ALVMILRRVL HFIAPVLTYT ADEAFRHSSA ALKGEYESIF
810 820 830 840 850
DLPKLSCAQG YNLEINEDFT RLLRVREKFT ESLDNLKKQK IVKSSLEVLL
860 870 880 890 900
QSPQEKEFAL LDRWLIVSGV CGKECKDKEE LARFMLEDKE EFVIYRAQNG
910 920 930
RCERCWQFIV EESEGKLCKR CTSVIAQTKK
Length:930
Mass (Da):106,646
Last modified:October 1, 2003 - v1
Checksum:iFBBDA80B50BE692E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017125 Genomic DNA. Translation: AAP77787.1.
RefSeqiNP_860721.1. NC_004917.1.
WP_011116030.1. NC_004917.1.

Genome annotation databases

EnsemblBacteriaiAAP77787; AAP77787; HH_1190.
GeneIDi1492878.
KEGGihhe:HH1190.
PATRICi20589834. VBIHelHep90276_1178.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017125 Genomic DNA. Translation: AAP77787.1 .
RefSeqi NP_860721.1. NC_004917.1.
WP_011116030.1. NC_004917.1.

3D structure databases

ProteinModelPortali Q7VGX8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 235279.HH1190.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAP77787 ; AAP77787 ; HH_1190 .
GeneIDi 1492878.
KEGGi hhe:HH1190.
PATRICi 20589834. VBIHelHep90276_1178.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci HHEP235279:GHUA-1226-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51449 / 3B1.

Entry informationi

Entry nameiSYI_HELHP
AccessioniPrimary (citable) accession number: Q7VGX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2003
Last modified: November 26, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3