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Q7VFE6

- BIOB_HELHP

UniProt

Q7VFE6 - BIOB_HELHP

Protein

Biotin synthase

Gene

bioB

Organism
Helicobacter hepaticus (strain ATCC 51449 / 3B1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi21 – 211Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi25 – 251Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi28 – 281Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi65 – 651Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi100 – 1001Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi225 – 2251Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciHHEP235279:GHUA-1771-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:HH_1731
    OrganismiHelicobacter hepaticus (strain ATCC 51449 / 3B1)
    Taxonomic identifieri235279 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
    ProteomesiUP000002495: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 283283Biotin synthasePRO_0000381423Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi235279.HH1731.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7VFE6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    KOiK01012.
    OMAiTCENTLR.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q7VFE6-1 [UniParc]FASTAAdd to Basket

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    MQKISNEIFL CSICNVSSGD CPEDCKYCTQ SAHYGTQIQR YKNKSIDKIV    50
    QEAKTLREYG ALGFCLVTAG RSLESQKCEY IAKAASAIKK ADLGLHIIAC 100
    CGSADVDSLK YLKTNGVDSY NHNLETSKEF FPHICTTHTW KERFETCENT 150
    LKAELGLLSG GIFGLGESWG DRIELLKHLQ ILSPHTSPLN FYIANESLPL 200
    PMQTLSPQEA LECVTLAREY LPNTRLMIAG GREAVFGDNQ KPLFEAGING 250
    VVLGDYLTTD GKAPKDDVAM IESYGYVAAT NCH 283
    Length:283
    Mass (Da):31,166
    Last modified:October 1, 2003 - v1
    Checksum:i84B9F5289D27EA69
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017125 Genomic DNA. Translation: AAP78328.1.
    RefSeqiNP_861262.1. NC_004917.1.
    WP_011116570.1. NC_004917.1.

    Genome annotation databases

    EnsemblBacteriaiAAP78328; AAP78328; HH_1731.
    GeneIDi1492278.
    KEGGihhe:HH1731.
    PATRICi20590898. VBIHelHep90276_1707.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017125 Genomic DNA. Translation: AAP78328.1 .
    RefSeqi NP_861262.1. NC_004917.1.
    WP_011116570.1. NC_004917.1.

    3D structure databases

    ProteinModelPortali Q7VFE6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 235279.HH1731.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP78328 ; AAP78328 ; HH_1731 .
    GeneIDi 1492278.
    KEGGi hhe:HH1731.
    PATRICi 20590898. VBIHelHep90276_1707.

    Phylogenomic databases

    eggNOGi COG0502.
    KOi K01012.
    OMAi TCENTLR.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci HHEP235279:GHUA-1771-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51449 / 3B1.

    Entry informationi

    Entry nameiBIOB_HELHP
    AccessioniPrimary (citable) accession number: Q7VFE6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3