Q7VFE6 (BIOB_HELHP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biotin synthase
EC=2.8.1.6

Gene names

Name:	bioB
Ordered Locus Names:	HH_1731

Organism

Helicobacter hepaticus (strain ATCC 51449 / 3B1) [Complete proteome] [HAMAP]

Taxonomic identifier

235279 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter hepaticus

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694
Catalytic activity	Dethiobiotin + sulfur + 2 S-adenosyl-L-methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the radical SAM superfamily. Biotin synthase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW biotin synthase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Biotin synthase HAMAP-Rule MF_01694		PRO_0000381423
Sites
	Metal binding	21	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	25	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	28	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	65	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	100	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	225	1	Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VFE6 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 84B9F5289D27EA69	FASTA	283	31,166
	10 20 30 40 50 60 MQKISNEIFL CSICNVSSGD CPEDCKYCTQ SAHYGTQIQR YKNKSIDKIV QEAKTLREYG 70 80 90 100 110 120 ALGFCLVTAG RSLESQKCEY IAKAASAIKK ADLGLHIIAC CGSADVDSLK YLKTNGVDSY 130 140 150 160 170 180 NHNLETSKEF FPHICTTHTW KERFETCENT LKAELGLLSG GIFGLGESWG DRIELLKHLQ 190 200 210 220 230 240 ILSPHTSPLN FYIANESLPL PMQTLSPQEA LECVTLAREY LPNTRLMIAG GREAVFGDNQ 250 260 270 280 KPLFEAGING VVLGDYLTTD GKAPKDDVAM IESYGYVAAT NCH

References

[1]

"The complete genome sequence of the carcinogenic bacterium Helicobacter hepaticus." Suerbaum S., Josenhans C., Sterzenbach T., Drescher B., Brandt P., Bell M., Droege M., Fartmann B., Fischer H.-P., Ge Z., Hoerster A., Holland R., Klein K., Koenig J., Macko L., Mendz G.L., Nyakatura G., Schauer D.B. , Shen Z., Weber J., Frosch M., Fox J.G. Proc. Natl. Acad. Sci. U.S.A. 100:7901-7906(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51449 / 3B1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017125 Genomic DNA. Translation: AAP78328.1.
RefSeq	NP_861262.1. NC_004917.1.
3D structure databases
HSSP	HSSP built from PDB template 1R30 based on UniProtKB P12996.
ProteinModelPortal	Q7VFE6.
ModBase	Search...
Protein-protein interaction databases
STRING	235279.HH1731.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAP78328; AAP78328; HH_1731.
GeneID	1492278.
KEGG	hhe:HH1731.
PATRIC	20590898. VBIHelHep90276_1707.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0502.
KO	K01012.
OMA	RIMMPAS.
ProtClustDB	PRK08508.
Enzyme and pathway databases
BioCyc	HHEP235279:GHUA-1732-MONOMER.
UniPathway	UPA00078; UER00162.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01694. BioB.
InterPro	IPR013785. Aldolase_TIM. IPR010722. Biotin/thiamin_synth-assoc. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM. [Graphical view]
Pfam	PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF001619. Biotin_synth. 1 hit.
SMART	SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00433. bioB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOB_HELHP

Accession

Primary (citable) accession number: Q7VFE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program