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Q7VFE6 (BIOB_HELHP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:HH_1731
OrganismHelicobacter hepaticus (strain ATCC 51449 / 3B1) [Complete proteome] [HAMAP]
Taxonomic identifier235279 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter

Protein attributes

Sequence length283 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 283283Biotin synthase HAMAP-Rule MF_01694
PRO_0000381423

Sites

Metal binding211Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding251Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding281Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding651Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1001Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2251Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7VFE6 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 84B9F5289D27EA69

FASTA28331,166
        10         20         30         40         50         60 
MQKISNEIFL CSICNVSSGD CPEDCKYCTQ SAHYGTQIQR YKNKSIDKIV QEAKTLREYG 

        70         80         90        100        110        120 
ALGFCLVTAG RSLESQKCEY IAKAASAIKK ADLGLHIIAC CGSADVDSLK YLKTNGVDSY 

       130        140        150        160        170        180 
NHNLETSKEF FPHICTTHTW KERFETCENT LKAELGLLSG GIFGLGESWG DRIELLKHLQ 

       190        200        210        220        230        240 
ILSPHTSPLN FYIANESLPL PMQTLSPQEA LECVTLAREY LPNTRLMIAG GREAVFGDNQ 

       250        260        270        280 
KPLFEAGING VVLGDYLTTD GKAPKDDVAM IESYGYVAAT NCH 

« Hide

References

[1]"The complete genome sequence of the carcinogenic bacterium Helicobacter hepaticus."
Suerbaum S., Josenhans C., Sterzenbach T., Drescher B., Brandt P., Bell M., Droege M., Fartmann B., Fischer H.-P., Ge Z., Hoerster A., Holland R., Klein K., Koenig J., Macko L., Mendz G.L., Nyakatura G., Schauer D.B. expand/collapse author list , Shen Z., Weber J., Frosch M., Fox J.G.
Proc. Natl. Acad. Sci. U.S.A. 100:7901-7906(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51449 / 3B1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017125 Genomic DNA. Translation: AAP78328.1.
RefSeqNP_861262.1. NC_004917.1.

3D structure databases

ProteinModelPortalQ7VFE6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING235279.HH1731.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP78328; AAP78328; HH_1731.
GeneID1492278.
KEGGhhe:HH1731.
PATRIC20590898. VBIHelHep90276_1707.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
KOK01012.
OMATCENTLR.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycHHEP235279:GHUA-1771-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_HELHP
AccessionPrimary (citable) accession number: Q7VFE6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: October 1, 2003
Last modified: May 14, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways