Reviewed, UniProtKB/Swiss-Prot Q7VEV7 (SYK2_MYCBO)

Names and origin

Protein names

Recommended name:
    Putative lysyl-tRNA synthetase 2
    EC=6.1.1.6
Alternative name(s):
    Lysine--tRNA ligase 2
      Short name=LysRS 2

Gene names

Name:	lysS2
Synonyms:	lysX
Ordered Locus Names:	Mb1667c

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	1172 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP MF_00252
Cofactor	Binds 3 magnesium ions per subunit By similarity. HAMAP MF_00252
Subcellular location	Cell membrane; Multi-pass membrane protein Potential HAMAP MF_00252.
Miscellaneous	There are two lysyl-tRNA ligases in M.bovis. HAMAP MF_00252
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP lysine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1172	1172	Putative lysyl-tRNA synthetase 2 HAMAP MF_00252		PRO_0000152656
Regions
	Transmembrane	80 – 100	21	Potential
	Transmembrane	122 – 142	21	Potential
	Transmembrane	146 – 166	21	Potential
	Transmembrane	177 – 197	21	Potential
	Transmembrane	214 – 234	21	Potential
	Transmembrane	272 – 292	21	Potential
	Region	1 – 663	663	Unknown HAMAP MF_00252
	Region	664 – 1172	509	Lysyl-tRNA synthetase HAMAP MF_00252
Sites
	Metal binding	1084	1	Magnesium 1 By similarity
	Metal binding	1091	1	Magnesium 1 By similarity
	Metal binding	1091	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VEV7-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 20B46091FC6FE926	FASTA	1,172	128,182
	10 20 30 40 50 60 MGLHLTVPGL RRDGRGVQSN SHDTSSKTTA DISRCPQHTD AGLQRAATPG ISRLLGISSR 70 80 90 100 110 120 SVTLTKPRSA TRGNSRYHWV PAAAGWTVGV IATLSLLASV SPLIRWIIKV PREFINDYLF 130 140 150 160 170 180 NFPDTNFAWS FVLALLAAAL TARKRIAWLV LLANMVLAAV VNAAEIAAGG NTAAESFGEN 190 200 210 220 230 240 LGFAVHVVAI VVLVLGYREF WAKVRRGALF RAAAVWLAGA VVGIVASWGL VELFPGSLAP 250 260 270 280 290 300 DERLGYAANR VVGFALADPD LFTGRPHVFL NAIFGLFGAF ALIGAAIVLF LSQRADNALT 310 320 330 340 350 360 GEDESAIRGL LDLYGKDDSL GYFATRRDKS VVFASSGRAC ITYRVEVGVC LASGDPVGDH 370 380 390 400 410 420 RAWPQAVDAW LRLCQTYGWA PGVMGASSQG AQTYREAGLT ALELGDEAIL RPADFKLSGP 430 440 450 460 470 480 EMRGVRQAVT RARRAGLTVR IRRHRDIAED EMAQTITRAD SWRDTETERG FSMALGRLGD 490 500 510 520 530 540 PADSDCLLVE AIDPHNQVLA MLSLVPWGTT GVSLDLMRRS PQSPNGTIEL MVSELALHAE 550 560 570 580 590 600 SLGITRISLN FAVFRAAFEQ GAQLGAGPVA RLWRGLLVFF SRWWQLETLY RSNMKYQPEW 610 620 630 640 650 660 VPRYACYEDA RVIPRVGVAS VIAEGFLVLP FSRRNRVHTG HHPAVPERLA ATGLLHHDGS 670 680 690 700 710 720 APDVSGLRQV GLTNGDGVER RLPEQVRVRF DKLEKLRSSG IDAFPVGRPP SHTVAQALAA 730 740 750 760 770 780 DHQASVSVSG RIMRIRNYGG VLFAQLRDWS GEMQVLLDNS RLDQGCAADF NAATDLGDLV 790 800 810 820 830 840 EMTGHMGASK TGTPSLIVSG WRLIGKCLRP LPNKWKGLLD PEARVRTRYL DLAVNAESRA 850 860 870 880 890 900 LITARSSVLR AVRETLFAKG FVEVETPILQ QLHGGATARP FVTHINTYSM DLFLRIAPEL 910 920 930 940 950 960 YLKRLCVGGV ERVFELGRAF RNEGVDFSHN PEFTLLEAYQ AHAGYLEWID GCRELIQNAA 970 980 990 1000 1010 1020 QAANGAPIAM RPRTDKGSDG TRHHLEPVDI SGIWPVRTVH DAISEALGER IDADTGLTTL 1030 1040 1050 1060 1070 1080 RKLCDAAGVP YRTQWDAGAV VLELYEHLVE CRTEQPTFYI DFPTSVSPLT RPHRSKRGVA 1090 1100 1110 1120 1130 1140 ERWDLVAWGI ELGTAYSELT DPVEQRRRLQ EQSLLAAGGD PEAMELDEDF LQAMEYAMPP 1150 1160 1170 TGGLGMGIDR VVMLITGRSI RETLPFPLAK PH

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248339 Genomic DNA. Translation: CAD96335.1.
RefSeq	NP_855320.1.
3D structure databases
SMR	Q7VEV7. Positions 298-608, 681-1171.
ModBase	Search...
Genome annotation databases
GeneID	1092607.
KEGG	mbo:Mb1667c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG431994.
OMA	ATRRDKA.
Enzyme and pathway databases
BioCyc	MBOV233413:MB1667C-MONOMER.
BRENDA	6.1.1.6. 3091.
Family and domain databases
HAMAP	MF_00252. Lys_tRNA_synth_class2. Fused. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-dom. IPR002313. Lys-tRNA-synth_II. IPR018149. Lys-tRNA-synth_II_C. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF4. tRNA-synt_lys_2. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR00982. TRNASYNTHLYS.
TIGRFAMs	TIGR00499. lysS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYK2_MYCBO

Accession

Primary (citable) accession number: Q7VEV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)