Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable cytosol aminopeptidase

Gene

pepA

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.UniRule annotation

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.UniRule annotation
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi279 – 2791Manganese 2UniRule annotation
Metal bindingi284 – 2841Manganese 1UniRule annotation
Metal bindingi284 – 2841Manganese 2UniRule annotation
Active sitei291 – 2911UniRule annotation
Metal bindingi302 – 3021Manganese 2UniRule annotation
Metal bindingi361 – 3611Manganese 1UniRule annotation
Metal bindingi363 – 3631Manganese 1UniRule annotation
Metal bindingi363 – 3631Manganese 2UniRule annotation
Active sitei365 – 3651UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosol aminopeptidaseUniRule annotation (EC:3.4.11.1UniRule annotation)
Alternative name(s):
Leucine aminopeptidaseUniRule annotation (EC:3.4.11.10UniRule annotation)
Short name:
LAPUniRule annotation
Leucyl aminopeptidaseUniRule annotation
Gene namesi
Name:pepAUniRule annotation
Synonyms:pepB
Ordered Locus Names:Mb2236
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 515515Probable cytosol aminopeptidasePRO_0000165765Add
BLAST

Proteomic databases

PRIDEiQ7VEN5.

Structurei

3D structure databases

ProteinModelPortaliQ7VEN5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000243129.
KOiK01255.
OMAiEGVQWAH.
OrthoDBiEOG6FV8B3.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7VEN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTEPGYLSP SVAVATSMPK RGVGAAVLIV PVVSTGEEDR PGAVVASAEP
60 70 80 90 100
FLRADTVAEI EAGLRALDAT GASDQVHRLA VPSLPVGSVL TVGLGKPRRE
110 120 130 140 150
WPADTIRCAA GVAARALNSS EAVITTLAEL PGDGICSATV EGLILGSYRF
160 170 180 190 200
SAFRSDKTAP KDAGLRKITV LCCAKDAKKR ALHGAAVATA VATARDLVNT
210 220 230 240 250
PPSHLFPAEL AKRAKTLSES VGLDVEVIDE KALKKAGYGG VIGVGQGSSR
260 270 280 290 300
PPRLVRLIHR GSRLAKNPQK AKKVALVGKG ITFDTGGISI KPAASMHHMT
310 320 330 340 350
SDMGGAAAVI ATVTLAARLR LPIDVIATVP MAENMPSATA QRPGDVLTQY
360 370 380 390 400
GGTTVEVLNT DAEGRLILAD AIVRACEDKP DYLIETSTLT GAQTVALGTR
410 420 430 440 450
IPGVMGSDEF RDRVAAISQR VGENGWPMPL PDDLKDDLKS TVADLANVSG
460 470 480 490 500
QRFAGMLVAG VFLREFVAES VDWAHIDVAG PAYNTGSAWG YTPKGATGVP
510
TRTMFAVLED IAKNG
Length:515
Mass (Da):53,447
Last modified:October 1, 2003 - v1
Checksum:iFEA76405AABC28E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO43490.1.
RefSeqiNP_855885.1. NC_002945.3.
WP_003411444.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCDO43490; CDO43490; Mb2236.
GeneIDi1091360.
KEGGimbo:Mb2236.
PATRICi18006612. VBIMycBov88188_2452.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO43490.1.
RefSeqiNP_855885.1. NC_002945.3.
WP_003411444.1. NC_002945.3.

3D structure databases

ProteinModelPortaliQ7VEN5.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ7VEN5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCDO43490; CDO43490; Mb2236.
GeneIDi1091360.
KEGGimbo:Mb2236.
PATRICi18006612. VBIMycBov88188_2452.

Phylogenomic databases

HOGENOMiHOG000243129.
KOiK01255.
OMAiEGVQWAH.
OrthoDBiEOG6FV8B3.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiAMPA_MYCBO
AccessioniPrimary (citable) accession number: Q7VEN5
Secondary accession number(s): X2BK52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: October 1, 2003
Last modified: November 11, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.