Q7VEK9 (PURL_PROMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Pro_0003

Organism

Prochlorococcus marinus (strain SARG / CCMP1375 / SS120) [Reference proteome] [HAMAP]

Taxonomic identifier

167539 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Prochlorales › Prochlorococcaceae › Prochlorococcus › Prochlorococcus marinus › Prochlorococcus marinus subsp. marinus

Protein attributes

Sequence length	793 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP-Rule MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP-Rule MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FGAMS family.
Sequence caution	The sequence AAP99049.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 793	793	Phosphoribosylformylglycinamidine synthase 2 HAMAP-Rule MF_00420		PRO_0000100477
Regions
	Nucleotide binding	110 – 121	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7VEK9 [UniParc]. Last modified June 7, 2005. Version 2. Checksum: DA470F1FED396B31	FASTA	793	86,331
	10 20 30 40 50 60 MIKSKDYLVD YNVELALKKE GLTKADYIEI CNRLKRSPNR TELGMFGVMW SEHCCYRNSR 70 80 90 100 110 120 SLLSNFPTTG KNILVGPGEN AGVVDLGEGQ RLAFKIESHN HPSAIEPFQG AATGVGGILR 130 140 150 160 170 180 DIFTMGARPI ALLNSLRFGP LDTPINVGLL EGVVSGIAHY GNCVGVPTVG GEVAFDKSYS 190 200 210 220 230 240 GNPLVNAMAL GIMETKEIVC SGAKGIDFPV IYVGSTTGRD GMGGASFASS ELTQASIDDR 250 260 270 280 290 300 PAVQVGDPFL EKGLIEGCLE AFKTGYVVAA QDMGAAGLTC SCSEMAAKGG VGIELDLDLV 310 320 330 340 350 360 PAREKNMTAY EFLLSESQER MLFVVEPGKE ELIMNKFRKW GLQAKVVGKV LEENIVRVIH 370 380 390 400 410 420 EKQIVVNLPA DSLAEDTPVN KHELLEEPPG FIKDHWKWDE TSLPKVSIKG VHFKENNTIL 430 440 450 460 470 480 DWNQIILRLL DDPTIASKRW IYNQYDYQVQ NNTIIAPGIG DAALIRLREI ENQNQNSNRG 490 500 510 520 530 540 IAAVVDCPNR WVALDPERGA IAAVAEASRN ISCVGAKPLA VTDNLNFSSP EDPIGYWQLA 550 560 570 580 590 600 KACKGLSKAC VVLETPVTGG NVSLYNETAL PNGLKQPIQP TPVVGMIGLI QDINSATRQG 610 620 630 640 650 660 WKDQGDQIYL LGTSIDPSIL NNQNISLAAT SYLENIYGLK TGRPPLIDLE FEKLVQLFLR 670 680 690 700 710 720 ESIANNLIKS AHDISDGGLV IGLAESCISS GLGIECNLPE IDNRLDKLLF AEGGSRVLVS 730 740 750 760 770 780 VSPNNIHNIK NSLNNFNIAN SEQISFNYLG TVTDNKYFQI NINQTKIIDL SVNEITNKFE 790 RSIPRRINST IVS

References

[1]

"Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a nearly minimal oxyphototrophic genome." Dufresne A., Salanoubat M., Partensky F., Artiguenave F., Axmann I.M., Barbe V., Duprat S., Galperin M.Y., Koonin E.V., Le Gall F., Makarova K.S., Ostrowski M., Oztas S., Robert C., Rogozin I.B., Scanlan D.J., Tandeau de Marsac N., Weissenbach J. , Wincker P., Wolf Y.I., Hess W.R. Proc. Natl. Acad. Sci. U.S.A. 100:10020-10025(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SARG / CCMP1375 / SS120.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017126 Genomic DNA. Translation: AAP99049.1. Different initiation.
RefSeq	NP_874397.1. NC_005042.1.
3D structure databases
ProteinModelPortal	Q7VEK9.
ModBase	Search...
Protein-protein interaction databases
STRING	167539.Pro0003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAP99049; AAP99049; Pro_0003.
GeneID	1461381.
KEGG	pma:Pro0003.
PATRIC	23026575. VBIProMar8617_0003.
Phylogenomic databases
eggNOG	COG0046.
KO	K01952.
OMA	WSEHCCY.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	PMAR167539:GJN2-3-MONOMER.
UniPathway	UPA00074; UER00128.
Family and domain databases
HAMAP	MF_00420. PurL_2.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_PROMA

Accession

Primary (citable) accession number: Q7VEK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	May 1, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program