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Q7VEK9

- PURL_PROMA

UniProt

Q7VEK9 - PURL_PROMA

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Protein

Phosphoribosylformylglycinamidine synthase subunit PurL

Gene

purL

Organism
Prochlorococcus marinus (strain SARG / CCMP1375 / SS120)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei53 – 531UniRule annotation
Binding sitei56 – 561ATPUniRule annotation
Binding sitei95 – 951ATPUniRule annotation
Metal bindingi97 – 971Magnesium 1UniRule annotation
Active sitei99 – 991Proton acceptorUniRule annotation
Binding sitei120 – 1201SubstrateUniRule annotation
Metal bindingi121 – 1211Magnesium 2UniRule annotation
Binding sitei244 – 2441SubstrateUniRule annotation
Metal bindingi272 – 2721Magnesium 2UniRule annotation
Binding sitei523 – 5231ATPUniRule annotation
Binding sitei560 – 5601ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi561 – 5611Magnesium 1UniRule annotation
Binding sitei563 – 5631SubstrateUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. phosphoribosylformylglycinamidine synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPMAR167539:GJN2-3-MONOMER.
UniPathwayiUPA00074; UER00128.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase subunit PurLUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase subunit IIUniRule annotation
Short name:
FGAR amidotransferase IIUniRule annotation
Short name:
FGAR-AT IIUniRule annotation
Glutamine amidotransferase PurLUniRule annotation
Phosphoribosylformylglycinamidine synthase subunit IIUniRule annotation
Gene namesi
Name:purLUniRule annotation
Ordered Locus Names:Pro_0003
OrganismiProchlorococcus marinus (strain SARG / CCMP1375 / SS120)
Taxonomic identifieri167539 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000001420: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 793793Phosphoribosylformylglycinamidine synthase subunit PurLPRO_0000100477Add
BLAST

Interactioni

Subunit structurei

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotation

Protein-protein interaction databases

STRINGi167539.Pro0003.

Structurei

3D structure databases

ProteinModelPortaliQ7VEK9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni98 – 1014Substrate bindingUniRule annotation
Regioni316 – 3183Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the FGAMS family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0046.
KOiK01952.
OMAiQAVVFKI.
OrthoDBiEOG6FNHHR.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
3.90.650.10. 1 hit.
HAMAPiMF_00420. PurL_2.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR010074. PRibForGlyAmidine_synth_II.
IPR016188. PurM_N-like.
[Graphical view]
PfamiPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMiSSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01736. FGAM_synth_II. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7VEK9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIKSKDYLVD YNVELALKKE GLTKADYIEI CNRLKRSPNR TELGMFGVMW
60 70 80 90 100
SEHCCYRNSR SLLSNFPTTG KNILVGPGEN AGVVDLGEGQ RLAFKIESHN
110 120 130 140 150
HPSAIEPFQG AATGVGGILR DIFTMGARPI ALLNSLRFGP LDTPINVGLL
160 170 180 190 200
EGVVSGIAHY GNCVGVPTVG GEVAFDKSYS GNPLVNAMAL GIMETKEIVC
210 220 230 240 250
SGAKGIDFPV IYVGSTTGRD GMGGASFASS ELTQASIDDR PAVQVGDPFL
260 270 280 290 300
EKGLIEGCLE AFKTGYVVAA QDMGAAGLTC SCSEMAAKGG VGIELDLDLV
310 320 330 340 350
PAREKNMTAY EFLLSESQER MLFVVEPGKE ELIMNKFRKW GLQAKVVGKV
360 370 380 390 400
LEENIVRVIH EKQIVVNLPA DSLAEDTPVN KHELLEEPPG FIKDHWKWDE
410 420 430 440 450
TSLPKVSIKG VHFKENNTIL DWNQIILRLL DDPTIASKRW IYNQYDYQVQ
460 470 480 490 500
NNTIIAPGIG DAALIRLREI ENQNQNSNRG IAAVVDCPNR WVALDPERGA
510 520 530 540 550
IAAVAEASRN ISCVGAKPLA VTDNLNFSSP EDPIGYWQLA KACKGLSKAC
560 570 580 590 600
VVLETPVTGG NVSLYNETAL PNGLKQPIQP TPVVGMIGLI QDINSATRQG
610 620 630 640 650
WKDQGDQIYL LGTSIDPSIL NNQNISLAAT SYLENIYGLK TGRPPLIDLE
660 670 680 690 700
FEKLVQLFLR ESIANNLIKS AHDISDGGLV IGLAESCISS GLGIECNLPE
710 720 730 740 750
IDNRLDKLLF AEGGSRVLVS VSPNNIHNIK NSLNNFNIAN SEQISFNYLG
760 770 780 790
TVTDNKYFQI NINQTKIIDL SVNEITNKFE RSIPRRINST IVS
Length:793
Mass (Da):86,331
Last modified:June 7, 2005 - v2
Checksum:iDA470F1FED396B31
GO

Sequence cautioni

The sequence AAP99049.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017126 Genomic DNA. Translation: AAP99049.1. Different initiation.
RefSeqiNP_874397.1. NC_005042.1.

Genome annotation databases

EnsemblBacteriaiAAP99049; AAP99049; Pro_0003.
GeneIDi1461381.
KEGGipma:Pro_0003.
PATRICi23026575. VBIProMar8617_0003.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017126 Genomic DNA. Translation: AAP99049.1 . Different initiation.
RefSeqi NP_874397.1. NC_005042.1.

3D structure databases

ProteinModelPortali Q7VEK9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 167539.Pro0003.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAP99049 ; AAP99049 ; Pro_0003 .
GeneIDi 1461381.
KEGGi pma:Pro_0003.
PATRICi 23026575. VBIProMar8617_0003.

Phylogenomic databases

eggNOGi COG0046.
KOi K01952.
OMAi QAVVFKI.
OrthoDBi EOG6FNHHR.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00128 .
BioCyci PMAR167539:GJN2-3-MONOMER.

Family and domain databases

Gene3Di 3.30.1330.10. 2 hits.
3.90.650.10. 1 hit.
HAMAPi MF_00420. PurL_2.
InterProi IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR010074. PRibForGlyAmidine_synth_II.
IPR016188. PurM_N-like.
[Graphical view ]
Pfami PF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view ]
SUPFAMi SSF55326. SSF55326. 2 hits.
SSF56042. SSF56042. 2 hits.
TIGRFAMsi TIGR01736. FGAM_synth_II. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SARG / CCMP1375 / SS120.

Entry informationi

Entry nameiPURL_PROMA
AccessioniPrimary (citable) accession number: Q7VEK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: October 29, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3