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Q7VDV0

- SYI_PROMA

UniProt

Q7VDV0 - SYI_PROMA

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Prochlorococcus marinus (strain SARG / CCMP1375 / SS120)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei584 – 5841Aminoacyl-adenylateUniRule annotation
Binding sitei628 – 6281ATPUniRule annotation
Metal bindingi938 – 9381ZincUniRule annotation
Metal bindingi941 – 9411ZincUniRule annotation
Metal bindingi958 – 9581ZincUniRule annotation
Metal bindingi961 – 9611ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMAR167539:GJN2-272-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Pro_0268
OrganismiProchlorococcus marinus (strain SARG / CCMP1375 / SS120)
Taxonomic identifieri167539 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000001420: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 969969Isoleucine--tRNA ligasePRO_0000098439Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi167539.Pro0268.

Structurei

3D structure databases

ProteinModelPortaliQ7VDV0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi68 – 7811"HIGH" regionAdd
BLAST
Motifi625 – 6295"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7VDV0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNHEKTNDGN NRPSYKESLN LLKTSFSMRA NAIVREPELQ QFWKEKGIDF
60 70 80 90 100
ELGLANKGST FTLHDGPPYA NGNLHMGHAL NKILKDIINK YQILKGSKVR
110 120 130 140 150
FVPGWDCHGL PIELQVLKTL DRKEREDLTP IKLRKKAAAY AHKQIALQKD
160 170 180 190 200
GFRRWGIWAD WDNPYLTLQK EYEAAQIKLF GEMAFKGYIY RGLKPVHWSP
210 220 230 240 250
SSRTALAEAE LEYPEGHTSP SIYVAFSVVK LPNLLQESLS KQGLALPVHE
260 270 280 290 300
DGLSNKLKVA IWTTTPWTLP ANMAVAVNEN IDYIIAKDNS EKLLIIASDL
310 320 330 340 350
REIVSEEIGS PLEKVAIVKG KMLDGVLYKH PLFDSLNPFV LGGNYITTES
360 370 380 390 400
GTGIVHTAPG HGVDDFNTGM KYKLPIICPV DEKGFFTSEA GDFEGLNVLK
410 420 430 440 450
DANTKIINAL KESGCLLKEK PYIHKYPYDW RTKKPTIFRA TEQWFASVEG
460 470 480 490 500
FRNDALNSIE KVEWLPQSGK KRIKSMVEDR GDWCISRQRN WGVPIPVFYS
510 520 530 540 550
KEGSKVLLNE ATIKHIYNLF VKYGADAWWE LPISELLPSN YSSEAHKWEK
560 570 580 590 600
GKDTMDVWFD SGSSWTSVIS KSDELNYPAD LYLEGSDQHR GWFQSSLLTS
610 620 630 640 650
VAVNSHAPYL KVLTHGFALD ENGRKMSKSL GNIIDPWKII NGGNNKKLEP
660 670 680 690 700
AYGADVLRLW VSSVDYSVDV PIGNNILSQL SDVYRKVRNT ARYLLGNLHD
710 720 730 740 750
FDPAKDGLDI GDLPILDRWM LNRTADVVDE ISIAFERYEF SKFFQLLQSF
760 770 780 790 800
CVVDLSNFYL DIAKDRLYVS APNDFRRRSC QYVLSLIVER LAGVISPVLS
810 820 830 840 850
HTAEDIWQNI PYKLNEESVF QRYWPKVPDS WRDSSFNKPI NQIRELRTSV
860 870 880 890 900
NRALEDCRTR QELGSSLEAS VRISPINESL QNSLTFLQEN GHNSVDNLFD
910 920 930 940 950
WLIVSKVQIG GEPWAEVLFT KEDDIGVIEI AKSRGAKCER CWHYELDIGQ
960
YERHPLLCGR CVDIIERID
Length:969
Mass (Da):110,344
Last modified:October 1, 2003 - v1
Checksum:i7D21237B13D0CB44
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017126 Genomic DNA. Translation: AAP99314.1.
RefSeqiNP_874662.1. NC_005042.1.
WP_011124423.1. NC_005042.1.

Genome annotation databases

EnsemblBacteriaiAAP99314; AAP99314; Pro_0268.
GeneIDi1461647.
KEGGipma:Pro_0268.
PATRICi23027127. VBIProMar8617_0276.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017126 Genomic DNA. Translation: AAP99314.1 .
RefSeqi NP_874662.1. NC_005042.1.
WP_011124423.1. NC_005042.1.

3D structure databases

ProteinModelPortali Q7VDV0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 167539.Pro0268.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAP99314 ; AAP99314 ; Pro_0268 .
GeneIDi 1461647.
KEGGi pma:Pro_0268.
PATRICi 23027127. VBIProMar8617_0276.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PMAR167539:GJN2-272-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SARG / CCMP1375 / SS120.

Entry informationi

Entry nameiSYI_PROMA
AccessioniPrimary (citable) accession number: Q7VDV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2003
Last modified: October 29, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3