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Q7VDU8 (TRMB_PROMA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Pro_0270
OrganismProchlorococcus marinus (strain SARG / CCMP1375 / SS120) [Reference proteome] [HAMAP]
Taxonomic identifier167539 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus

Protein attributes

Sequence length213 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 213213tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171373

Sites

Active site1131 By similarity
Binding site351S-adenosyl-L-methionine By similarity
Binding site601S-adenosyl-L-methionine By similarity
Binding site871S-adenosyl-L-methionine By similarity
Binding site1131S-adenosyl-L-methionine By similarity
Binding site1171Substrate By similarity
Binding site1491Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7VDU8 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 6413A68BC374A5DC

FASTA21325,154
        10         20         30         40         50         60 
MRQHVNPLSH FFQLHNELPR PEDLFESIDL PIHLDIGCAR GKFLIDMAAD NAQWNFLGVE 

        70         80         90        100        110        120 
IREPLVFAAE KERIELGLTN LRFFFCNANI SLEKWFLSLE NGQLKRVSIQ FPDPWFKKRH 

       130        140        150        160        170        180 
RKRRVLNQSF LFLLAQSLDI GSELFIQSDV ISVMDEMTEI IELSHFFDRH SKYSESNLNA 

       190        200        210 
NPFKFCTERE KYAIHQGVKV YRQLYFRNST GIL 

« Hide

References

[1]"Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a nearly minimal oxyphototrophic genome."
Dufresne A., Salanoubat M., Partensky F., Artiguenave F., Axmann I.M., Barbe V., Duprat S., Galperin M.Y., Koonin E.V., Le Gall F., Makarova K.S., Ostrowski M., Oztas S., Robert C., Rogozin I.B., Scanlan D.J., Tandeau de Marsac N., Weissenbach J. expand/collapse author list , Wincker P., Wolf Y.I., Hess W.R.
Proc. Natl. Acad. Sci. U.S.A. 100:10020-10025(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SARG / CCMP1375 / SS120.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017126 Genomic DNA. Translation: AAP99316.1.
RefSeqNP_874664.1. NC_005042.1.

3D structure databases

ProteinModelPortalQ7VDU8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING167539.Pro0270.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP99316; AAP99316; Pro_0270.
GeneID1461649.
KEGGpma:Pro_0270.
PATRIC23027131. VBIProMar8617_0278.

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OMAHVNPLSR.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycPMAR167539:GJN2-274-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_PROMA
AccessionPrimary (citable) accession number: Q7VDU8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: October 1, 2003
Last modified: April 16, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways