Q7VDB2 (SYE_PROMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Pro_0471

Organism

Prochlorococcus marinus (strain SARG / CCMP1375 / SS120)

Taxonomic identifier

167539 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000119624
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00022_B
	Motif	248 – 252	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	251	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VDB2 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 0FDDFD8035A9F910	FASTA	476	54,477
	10 20 30 40 50 60 MKVRVRLAPS PTGSLHIGTA RTALFNWLFA QRNKGSFLIR IEDTDKERSK DEFTSNILEG 70 80 90 100 110 120 LQWLGLKWDE APIIQSKRIH EHKEAIKSLI NKGLAYRCFA TEEELQTMRD AQINSGEAPR 130 140 150 160 170 180 YDNRHRKLTK EQEQKFIKDG RSAVIRFRIE DEESVHWNDM VRGPIHWNSK DLGGDMVISR 190 200 210 220 230 240 RAPANEIGDP LYNLVVVLDD AAMEITHVIR GEDHIANTAK QLLLYKALGF KEPSFAHTPL 250 260 270 280 290 300 ILNKDGRKLS KRDGVTSISD FKTMGYTSQA MTNYMTLLGW SPPEGMGEKF TLKESSSVFD 310 320 330 340 350 360 FDRVNNAGAK FDWDKLQWLN SQEIHNWSNE KLLDSLIPLW TKEGWNLSSK DWGLKLVHLI 370 380 390 400 410 420 KPSLILINDG IEESRVFFEE PLLKKDAIQQ LEIPRAKEAL KFICRKLEET PWHGKENEIA 430 440 450 460 470 IELITSCSKS LDIKKGIIMK SLRAALLGTL QGPDLITSWG LLAMMENDQA RIKRCL

References

[1]

"Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a nearly minimal oxyphototrophic genome." Dufresne A., Salanoubat M., Partensky F., Artiguenave F., Axmann I.M., Barbe V., Duprat S., Galperin M.Y., Koonin E.V., Le Gall F., Makarova K.S., Ostrowski M., Oztas S., Robert C., Rogozin I.B., Scanlan D.J., Tandeau de Marsac N., Weissenbach J. , Wincker P., Wolf Y.I., Hess W.R. Proc. Natl. Acad. Sci. U.S.A. 100:10020-10025(2003) [PubMed: 12917486] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SARG / CCMP1375 / SS120.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017126 Genomic DNA. Translation: AAP99516.1.
RefSeq	NP_874864.1. NC_005042.1.
3D structure databases
ProteinModelPortal	Q7VDB2.
SMR	Q7VDB2. Positions 2-476.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1461853.
GenomeReviews	Gene locus Pro_0471 in contig AE017126_GR.
KEGG	pma:Pro0471.
PATRIC	23027567. VBIProMar8617_0484.
Phylogenomic databases
HOGENOM	HBG628189.
OMA	DKETAND.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	PMAR167539:PRO_0471-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_PROMA

Accession

Primary (citable) accession number: Q7VDB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program