Q7VD23 (GLO2_PROMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	Pro_0561

Organism

Prochlorococcus marinus (strain SARG / CCMP1375 / SS120)

Taxonomic identifier

167539 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_0000309675
Sites
	Metal binding	59	1	Zinc 1 By similarity
	Metal binding	61	1	Zinc 1 By similarity
	Metal binding	63	1	Zinc 2 By similarity
	Metal binding	64	1	Zinc 2 By similarity
	Metal binding	118	1	Zinc 1 By similarity
	Metal binding	143	1	Zinc 1 By similarity
	Metal binding	143	1	Zinc 2 By similarity
	Metal binding	181	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VD23 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 501C275FBFCA9E73	FASTA	253	28,879
	10 20 30 40 50 60 MKKKEEGFII HPIPVLMDNI IWIWVKEKQA IVVDPAISEP VINLLKGNGL SLHSVLQTHH 70 80 90 100 110 120 HEDHIGGTQE LINVWPSASV IAAKSDLDRI QFQTKSVVDN EELDILGQKI KVIEVPGHTS 130 140 150 160 170 180 NHICFFLQGS KESKIDPVLF CGDTLFGAGC GRLFEGTPEQ MFNSLSRINN LPKNTKIYCA 190 200 210 220 230 240 HEYTEANLRW AKSIFPEDIY IEERLKEVIL RKSKGFLSIP SKLSEERKTN LFIRASNQRE 250 FAQLRLHKDN WKS

References

[1]

"Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a nearly minimal oxyphototrophic genome." Dufresne A., Salanoubat M., Partensky F., Artiguenave F., Axmann I.M., Barbe V., Duprat S., Galperin M.Y., Koonin E.V., Le Gall F., Makarova K.S., Ostrowski M., Oztas S., Robert C., Rogozin I.B., Scanlan D.J., Tandeau de Marsac N., Weissenbach J. , Wincker P., Wolf Y.I., Hess W.R. Proc. Natl. Acad. Sci. U.S.A. 100:10020-10025(2003) [PubMed: 12917486] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SARG / CCMP1375 / SS120.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017126 Genomic DNA. Translation: AAP99606.1.
RefSeq	NP_874954.1. NC_005042.1.
3D structure databases
HSSP	HSSP built from PDB template 1XM8 based on UniProtKB Q9SID3.
ProteinModelPortal	Q7VD23.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1461943.
GenomeReviews	Gene locus Pro_0561 in contig AE017126_GR.
KEGG	pma:Pro0561.
PATRIC	23027755. VBIProMar8617_0576.
Phylogenomic databases
HOGENOM	HBG753931.
OMA	FLCRTDN.
ProtClustDB	CLSK919284.
Enzyme and pathway databases
BioCyc	PMAR167539:PRO_0561-MONOMER.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_PROMA

Accession

Primary (citable) accession number: Q7VD23

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program