Q7VCH6 (ISPE_PROMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Pro_0764

Organism

Prochlorococcus marinus (strain SARG / CCMP1375 / SS120)

Taxonomic identifier

167539 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000189246
Regions
	Nucleotide binding	106 – 116	11	ATP Potential
Sites
	Active site	21	1	By similarity
	Active site	148	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VCH6 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 3C5FE95D3B8CA93F	FASTA	319	34,635
	10 20 30 40 50 60 MNINYSELSS SRSLSVIAPA KINLHLEVLG FRKDGFHELA MLMQSINLFD EIDFLKTDNG 70 80 90 100 110 120 EITLTSDDPN LSTGDDNLIL KAAQLIQTCS SDKNVGAEIH LKKNIPIGAG LAGGSSDAAA 130 140 150 160 170 180 TLVGLNSLWG IGSSEKQLEK LGSELGSDVP FCLRGGTQFC FGRGESLEMI PEIKQSMAVV 190 200 210 220 230 240 LVKDPLVEVS TPWAYSKFKE IYGNDYLKME EDFEKRRQSL RDASWLNPLN CTNPPPLQND 250 260 270 280 290 300 LQKVIEPITP AVRNALEFLS SLEGVLSLAM SGSGPSCFGI FADLNGAQIA LEENRNKLKL 310 SGLDAWCCAF KSSGVSLRL

References

[1]

"Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a nearly minimal oxyphototrophic genome." Dufresne A., Salanoubat M., Partensky F., Artiguenave F., Axmann I.M., Barbe V., Duprat S., Galperin M.Y., Koonin E.V., Le Gall F., Makarova K.S., Ostrowski M., Oztas S., Robert C., Rogozin I.B., Scanlan D.J., Tandeau de Marsac N., Weissenbach J. , Wincker P., Wolf Y.I., Hess W.R. Proc. Natl. Acad. Sci. U.S.A. 100:10020-10025(2003) [PubMed: 12917486] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SARG / CCMP1375 / SS120.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017126 Genomic DNA. Translation: AAP99808.1.
RefSeq	NP_875156.1. NC_005042.1.
3D structure databases
ProteinModelPortal	Q7VCH6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1462146.
GenomeReviews	Gene locus Pro_0764 in contig AE017126_GR.
KEGG	pma:Pro0764.
PATRIC	23028229. VBIProMar8617_0808.
Phylogenomic databases
HOGENOM	HBG734593.
OMA	QLCFGRG.
ProtClustDB	PRK02534.
Enzyme and pathway databases
BioCyc	PMAR167539:PRO_0764-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_PROMA

Accession

Primary (citable) accession number: Q7VCH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program