Reviewed, UniProtKB/Swiss-Prot Q7VBV0 (ACSF_PROMA)

Names and origin

Protein names

Recommended name:
    Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase
      Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase
    EC=1.14.13.81

Gene names

Name:	acsF
Ordered Locus Names:	Pro_0992

Organism

Prochlorococcus marinus [Complete proteome] [HAMAP]

Taxonomic identifier

1219 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity.
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + H(2)O. 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + 2 H(2)O. 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = divinylprotochlorophyllide + NADP(+) + 2 H(2)O.
Cofactor	Iron By similarity.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis (light-independent).
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: HAMAP magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase		PRO_0000217530

Sequences

	Sequence		Length	Mass (Da)
	Q7VBV0-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: AAACDF2B53AAA97E	FASTA	347	40,465
	10 20 30 40 50 60 MTTTTSGAPA SLGRNELPPH LDENLLTPRF YTTEFEKAAK TDLEIARKDF EAMFKEMEAD 70 80 90 100 110 120 YNLKHFDRKA SLERLDELSP EDKAVYESYL VRSVVSEFSG FLLFKEISNR FKKAGRQELG 130 140 150 160 170 180 QFFTFLARDE ARHAGFLGRA LKTEGINVDL PNLPKKRAAT FFPLSWVLYS LYLSEKIGYW 190 200 210 220 230 240 RYILINRHLK ANPDKVCAPL FDFFEPWCQD ENRHGDCINL MMRCWPGMTK GFRGKLLSRF 250 260 270 280 290 300 FLWTVFLTHT LTVCERGEFY ELLGIDPVLF DEEVIIQTNN TSKNAFPWVY KFEDGKFLSM 310 320 330 340 RIDILNAFKK WRNQNGIKKP LALGKFVLLI LKQFTLPMEK TDAVRYG

References

[1]

"Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a nearly minimal oxyphototrophic genome." Dufresne A., Salanoubat M., Partensky F., Artiguenave F., Axmann I.M., Barbe V., Duprat S., Galperin M.Y., Koonin E.V., Le Gall F., Makarova K.S., Ostrowski M., Oztas S., Robert C., Rogozin I.B., Scanlan D.J., Tandeau de Marsac N., Weissenbach J. , Wincker P., Wolf Y.I., Hess W.R. Proc. Natl. Acad. Sci. U.S.A. 100:10020-10025(2003) [PubMed: 12917486] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SARG / CCMP1375 / SS120.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017126 Genomic DNA. Translation: AAQ00037.1.
RefSeq	NP_875384.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1462374.
GenomeReviews	Gene locus Pro_0992 in contig AE017126_GR.
KEGG	pma:Pro0992.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7VBV0.
Enzyme and pathway databases
BioCyc	PMAR167539:PRO_0992-MON.
Family and domain databases
HAMAP	MF_01840. [Tree]
InterPro	IPR008434. AcsF. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACSF_PROMA

Accession

Primary (citable) accession number: Q7VBV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	October 1, 2003
Last modified:	November 25, 2008
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)