Reviewed, UniProtKB/Swiss-Prot Q7VA47 (FUMC_PROMA)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class II
      Short name=Fumarase C
    EC=4.2.1.2

Gene names

Name:	fumC
Ordered Locus Names:	Pro_1620

Organism

Prochlorococcus marinus [Complete proteome] [HAMAP]

Taxonomic identifier

1219 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle. HAMAP MF_00743
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161296
Regions
	Region	130 – 133	4	B site By similarity
	Region	140 – 142	3	Substrate binding By similarity
Sites
	Binding site	101	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7VA47-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: F2F17242168FDA54	FASTA	465	51,045
	10 20 30 40 50 60 MAQSFRIEND SMGTIKVPDQ ALWGAQTQRS LINFAIGHNK MPMKLIYSIV QIKASAAIVN 70 80 90 100 110 120 CRLGVLDKQR KNFILNACNE ISNGMHDEQF PLSVWQTGSG TQTNMNVNEV ISNIASHLNG 130 140 150 160 170 180 NKLGSHEPLH PNDHVNRSQS TNDVFPAAIQ IATVQEILEN LLPELDQLIE TFDKKIIKWN 190 200 210 220 230 240 RIIKTGRTHL QDAVPLTLGQ EASAWKEQLI ASRNRLNKSL NELYPLPLGG TAIGTGLNAP 250 260 270 280 290 300 AKFDKEIALE IAKSTRSPFV SAQNKFAIMA SHDALVHTMS QLKLLAVSLF KIVNDLRLLS 310 320 330 340 350 360 CGPRGGLGEL RLPENEPGSS IMPGKVNPTQ CEAMAMVCTQ IMALDSAVTM AGSGGHLQMN 370 380 390 400 410 420 SYKPLIAFNL LESIDLLSSA CKSSRILMIE GIEPNLEKIQ NSLQNSLMLI TSLTPIIGYE 430 440 450 460 KASKIAQCAH EKDITLKEAT KLLGYLNEDD FDRIVNPQSM TGMEN

References

[1]

"Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a nearly minimal oxyphototrophic genome." Dufresne A., Salanoubat M., Partensky F., Artiguenave F., Axmann I.M., Barbe V., Duprat S., Galperin M.Y., Koonin E.V., Le Gall F., Makarova K.S., Ostrowski M., Oztas S., Robert C., Rogozin I.B., Scanlan D.J., Tandeau de Marsac N., Weissenbach J. , Wincker P., Wolf Y.I., Hess W.R. Proc. Natl. Acad. Sci. U.S.A. 100:10020-10025(2003) [PubMed: 12917486] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SARG / CCMP1375 / SS120.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017126 Genomic DNA. Translation: AAQ00664.1.
RefSeq	NP_876011.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1463002.
GenomeReviews	Gene locus Pro_1620 in contig AE017126_GR.
KEGG	pma:Pro1620.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7VA47.
OMA	GSQGHFE.
Enzyme and pathway databases
BioCyc	PMAR167539:PRO_1620-MON.
BRENDA	4.2.1.2. 141456.
Family and domain databases
HAMAP	MF_00743. [Tree]
InterPro	IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00149. FUMRATELYASE.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_PROMA

Accession

Primary (citable) accession number: Q7VA47

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)