Q7V8X5 (MTND_PROMM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acireductone dioxygenase

Alternative name(s):
1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Short name=DHK-MTPene dioxygenase
Acireductone dioxygenase (Fe(2+)-requiring)
Short name=ARD'
Short name=Fe-ARD
EC=1.13.11.54
Acireductone dioxygenase (Ni(2+)-requiring)
Short name=ARD
Short name=Ni-ARD
EC=1.13.11.53

Gene names

Name:	mtnD
Ordered Locus Names:	PMT_0197

Organism

Prochlorococcus marinus (strain MIT 9313) [Complete proteome] [HAMAP]

Taxonomic identifier

74547 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Prochlorales › Prochlorococcaceae › Prochlorococcus › Prochlorococcus marinus

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway By similarity. HAMAP-Rule MF_01682
Catalytic activity	1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 3-(methylthio)propanoate + formate + CO. HAMAP-Rule MF_01682 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 4-(methylthio)-2-oxobutanoate + formate.
Cofactor	Binds 1 iron ion per monomer By similarity. HAMAP-Rule MF_01682 Binds 1 nickel ion per monomer By similarity. HAMAP-Rule MF_01682
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6. HAMAP-Rule MF_01682
Subunit structure	Monomer By similarity. HAMAP-Rule MF_01682
Sequence similarities	Belongs to the acireductone dioxygenase (ARD) family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Iron Metal-binding Nickel
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-methionine biosynthetic process from S-adenosylmethionine Inferred from electronic annotation. Source: HAMAP L-methionine salvage from methylthioadenosine Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	acireductone dioxygenase (Ni2+-requiring) activity Inferred from electronic annotation. Source: HAMAP acireductone dioxygenase [iron(II)-requiring] activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP nickel cation binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 185	185	Acireductone dioxygenase HAMAP-Rule MF_01682		PRO_0000359216
Sites
	Metal binding	102	1	Iron; alternate By similarity
	Metal binding	102	1	Nickel; alternate By similarity
	Metal binding	104	1	Iron; alternate By similarity
	Metal binding	104	1	Nickel; alternate By similarity
	Metal binding	108	1	Iron; alternate By similarity
	Metal binding	108	1	Nickel; alternate By similarity
	Metal binding	146	1	Iron; alternate By similarity
	Metal binding	146	1	Nickel; alternate By similarity
	Site	101	1	May play a role in metal incorporation in vivo By similarity
	Site	107	1	May play a role in transmitting local conformational changes By similarity
	Site	110	1	Important to generate the dianion By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7V8X5 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 7F642F7459F8E8C7	FASTA	185	20,649
	10 20 30 40 50 60 MSRLSIHPEG STNATSPAEP LLESDDPAVI KVELAKRGIA FQRWPAKVKL DQNSSESDIL 70 80 90 100 110 120 AAYAVEIARV QADGRYPTVD AIRITPDHPD REALRQKFLD EHTHAEDEVR FFVEGCGLFC 130 140 150 160 170 180 LHIGAEVLQV LCEQNDCINV PAGTRHWFDM GSKPQFCAVR FFDNPEGWIA NFTGDAIAER FAKLP

References

[1]

"Genome divergence in two Prochlorococcus ecotypes reflects oceanic niche differentiation." Rocap G., Larimer F.W., Lamerdin J.E., Malfatti S., Chain P., Ahlgren N.A., Arellano A., Coleman M., Hauser L., Hess W.R., Johnson Z.I., Land M.L., Lindell D., Post A.F., Regala W., Shah M., Shaw S.L., Steglich C. , Sullivan M.B., Ting C.S., Tolonen A., Webb E.A., Zinser E.R., Chisholm S.W. Nature 424:1042-1047(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MIT 9313.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX548175 Genomic DNA. Translation: CAE20372.1.
RefSeq	NP_894030.1. NC_005071.1.
3D structure databases
ProteinModelPortal	Q7V8X5.
SMR	Q7V8X5. Positions 2-185.
ModBase	Search...
Protein-protein interaction databases
STRING	74547.PMT0197.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAE20372; CAE20372; PMT_0197.
GeneID	1729659.
KEGG	pmt:PMT0197.
PATRIC	23008113. VBIProMar135351_0217.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1791.
KO	K08967.
OMA	VLCEKND.
ProtClustDB	CLSK825846.
Enzyme and pathway databases
UniPathway	UPA00904; UER00878.
Family and domain databases
Gene3D	2.60.120.10. 1 hit.
HAMAP	MF_01682. Salvage_MtnD.
InterPro	IPR023956. Acireductn_d0ase. IPR004313. Acireductn_dOase_family. IPR014710. RmlC-like_jellyroll. IPR011051. RmlC_Cupin. [Graphical view]
PANTHER	PTHR23418. PTHR23418. 1 hit.
Pfam	PF03079. ARD. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTND_PROMM

Accession

Primary (citable) accession number: Q7V8X5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program