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Q7V8X5

- MTND_PROMM

UniProt

Q7V8X5 - MTND_PROMM

Protein

Acireductone dioxygenase

Gene

mtnD

Organism
Prochlorococcus marinus (strain MIT 9313)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.UniRule annotation

    Catalytic activityi

    1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 3-(methylthio)propanoate + formate + CO.UniRule annotation
    1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 4-(methylthio)-2-oxobutanoate + formate.UniRule annotation

    Cofactori

    Binds 1 iron ion per monomer.UniRule annotation
    Binds 1 nickel ion per monomer.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei101 – 1011May play a role in metal incorporation in vivoUniRule annotation
    Metal bindingi102 – 1021Iron; alternateUniRule annotation
    Metal bindingi102 – 1021Nickel; alternateUniRule annotation
    Metal bindingi104 – 1041Iron; alternateUniRule annotation
    Metal bindingi104 – 1041Nickel; alternateUniRule annotation
    Sitei107 – 1071May play a role in transmitting local conformational changesUniRule annotation
    Metal bindingi108 – 1081Iron; alternateUniRule annotation
    Metal bindingi108 – 1081Nickel; alternateUniRule annotation
    Sitei110 – 1101Important to generate the dianionUniRule annotation
    Metal bindingi146 – 1461Iron; alternateUniRule annotation
    Metal bindingi146 – 1461Nickel; alternateUniRule annotation

    GO - Molecular functioni

    1. acireductone dioxygenase (Ni2+-requiring) activity Source: UniProtKB-HAMAP
    2. acireductone dioxygenase [iron(II)-requiring] activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. nickel cation binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. L-methionine biosynthetic process from methylthioadenosine Source: UniProtKB-UniPathway
    2. L-methionine biosynthetic process from S-adenosylmethionine Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Methionine biosynthesis

    Keywords - Ligandi

    Iron, Metal-binding, Nickel

    Enzyme and pathway databases

    UniPathwayiUPA00904; UER00878.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acireductone dioxygenaseUniRule annotation
    Alternative name(s):
    1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenaseUniRule annotation
    Short name:
    DHK-MTPene dioxygenaseUniRule annotation
    Acireductone dioxygenase (Fe(2+)-requiring)UniRule annotation (EC:1.13.11.54UniRule annotation)
    Short name:
    ARD'UniRule annotation
    Short name:
    Fe-ARDUniRule annotation
    Acireductone dioxygenase (Ni(2+)-requiring)UniRule annotation (EC:1.13.11.53UniRule annotation)
    Short name:
    ARDUniRule annotation
    Short name:
    Ni-ARDUniRule annotation
    Gene namesi
    Name:mtnDUniRule annotation
    Ordered Locus Names:PMT_0197
    OrganismiProchlorococcus marinus (strain MIT 9313)
    Taxonomic identifieri74547 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
    ProteomesiUP000001423: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 185185Acireductone dioxygenasePRO_0000359216Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi74547.PMT0197.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7V8X5.
    SMRiQ7V8X5. Positions 2-185.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the acireductone dioxygenase (ARD) family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1791.
    KOiK08967.
    OMAiVLCEKND.
    OrthoDBiEOG6ZPT06.

    Family and domain databases

    Gene3Di2.60.120.10. 1 hit.
    HAMAPiMF_01682. Salvage_MtnD.
    InterProiIPR023956. Acireductn_d0ase.
    IPR004313. Acireductn_dOase_family.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    [Graphical view]
    PANTHERiPTHR23418. PTHR23418. 1 hit.
    PfamiPF03079. ARD. 1 hit.
    [Graphical view]
    SUPFAMiSSF51182. SSF51182. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q7V8X5-1 [UniParc]FASTAAdd to Basket

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    MSRLSIHPEG STNATSPAEP LLESDDPAVI KVELAKRGIA FQRWPAKVKL    50
    DQNSSESDIL AAYAVEIARV QADGRYPTVD AIRITPDHPD REALRQKFLD 100
    EHTHAEDEVR FFVEGCGLFC LHIGAEVLQV LCEQNDCINV PAGTRHWFDM 150
    GSKPQFCAVR FFDNPEGWIA NFTGDAIAER FAKLP 185
    Length:185
    Mass (Da):20,649
    Last modified:October 1, 2003 - v1
    Checksum:i7F642F7459F8E8C7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX548175 Genomic DNA. Translation: CAE20372.1.
    RefSeqiNP_894030.1. NC_005071.1.

    Genome annotation databases

    EnsemblBacteriaiCAE20372; CAE20372; PMT_0197.
    GeneIDi1729659.
    KEGGipmt:PMT0197.
    PATRICi23008113. VBIProMar135351_0217.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX548175 Genomic DNA. Translation: CAE20372.1 .
    RefSeqi NP_894030.1. NC_005071.1.

    3D structure databases

    ProteinModelPortali Q7V8X5.
    SMRi Q7V8X5. Positions 2-185.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 74547.PMT0197.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAE20372 ; CAE20372 ; PMT_0197 .
    GeneIDi 1729659.
    KEGGi pmt:PMT0197.
    PATRICi 23008113. VBIProMar135351_0217.

    Phylogenomic databases

    eggNOGi COG1791.
    KOi K08967.
    OMAi VLCEKND.
    OrthoDBi EOG6ZPT06.

    Enzyme and pathway databases

    UniPathwayi UPA00904 ; UER00878 .

    Family and domain databases

    Gene3Di 2.60.120.10. 1 hit.
    HAMAPi MF_01682. Salvage_MtnD.
    InterProi IPR023956. Acireductn_d0ase.
    IPR004313. Acireductn_dOase_family.
    IPR014710. RmlC-like_jellyroll.
    IPR011051. RmlC_Cupin.
    [Graphical view ]
    PANTHERi PTHR23418. PTHR23418. 1 hit.
    Pfami PF03079. ARD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51182. SSF51182. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MIT 9313.

    Entry informationi

    Entry nameiMTND_PROMM
    AccessioniPrimary (citable) accession number: Q7V8X5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 20, 2009
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3