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Protein

Acireductone dioxygenase

Gene

mtnD

Organism
Prochlorococcus marinus (strain MIT 9313)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.UniRule annotation

Catalytic activityi

1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 3-(methylthio)propanoate + formate + CO.UniRule annotation
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 4-(methylthio)-2-oxobutanoate + formate.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Fe cationUniRule annotationNote: Binds 1 Fe cation per monomer.UniRule annotation
  • Ni2+UniRule annotationNote: Binds 1 nickel ion per monomer.UniRule annotation

Pathway:iL-methionine biosynthesis via salvage pathway

This protein is involved in step 5 of the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Methylthioribose-1-phosphate isomerase (mtnA)
  2. Methylthioribulose-1-phosphate dehydratase (mtnB)
  3. Enolase-phosphatase E1 (mtnC)
  4. Enolase-phosphatase E1 (mtnC)
  5. Acireductone dioxygenase (mtnD)
  6. no protein annotated in this organism
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate, the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei101 – 1011May play a role in metal incorporation in vivoUniRule annotation
Metal bindingi102 – 1021Iron; alternateUniRule annotation
Metal bindingi102 – 1021Nickel; alternateUniRule annotation
Metal bindingi104 – 1041Iron; alternateUniRule annotation
Metal bindingi104 – 1041Nickel; alternateUniRule annotation
Sitei107 – 1071May play a role in transmitting local conformational changesUniRule annotation
Metal bindingi108 – 1081Iron; alternateUniRule annotation
Metal bindingi108 – 1081Nickel; alternateUniRule annotation
Sitei110 – 1101Important to generate the dianionUniRule annotation
Metal bindingi146 – 1461Iron; alternateUniRule annotation
Metal bindingi146 – 1461Nickel; alternateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Iron, Metal-binding, Nickel

Enzyme and pathway databases

UniPathwayiUPA00904; UER00878.

Names & Taxonomyi

Protein namesi
Recommended name:
Acireductone dioxygenaseUniRule annotation
Alternative name(s):
1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenaseUniRule annotation
Short name:
DHK-MTPene dioxygenaseUniRule annotation
Acireductone dioxygenase (Fe(2+)-requiring)UniRule annotation (EC:1.13.11.54UniRule annotation)
Short name:
ARD'UniRule annotation
Short name:
Fe-ARDUniRule annotation
Acireductone dioxygenase (Ni(2+)-requiring)UniRule annotation (EC:1.13.11.53UniRule annotation)
Short name:
ARDUniRule annotation
Short name:
Ni-ARDUniRule annotation
Gene namesi
Name:mtnDUniRule annotation
Ordered Locus Names:PMT_0197
OrganismiProchlorococcus marinus (strain MIT 9313)
Taxonomic identifieri74547 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000001423 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 185185Acireductone dioxygenasePRO_0000359216Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi74547.PMT0197.

Structurei

3D structure databases

ProteinModelPortaliQ7V8X5.
SMRiQ7V8X5. Positions 2-185.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acireductone dioxygenase (ARD) family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1791.
KOiK08967.
OMAiWFDMGSE.
OrthoDBiEOG6ZPT06.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_01682. Salvage_MtnD.
InterProiIPR023956. Acireductn_d0ase.
IPR004313. Acireductn_dOase_family.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR23418. PTHR23418. 1 hit.
PfamiPF03079. ARD. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7V8X5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRLSIHPEG STNATSPAEP LLESDDPAVI KVELAKRGIA FQRWPAKVKL
60 70 80 90 100
DQNSSESDIL AAYAVEIARV QADGRYPTVD AIRITPDHPD REALRQKFLD
110 120 130 140 150
EHTHAEDEVR FFVEGCGLFC LHIGAEVLQV LCEQNDCINV PAGTRHWFDM
160 170 180
GSKPQFCAVR FFDNPEGWIA NFTGDAIAER FAKLP
Length:185
Mass (Da):20,649
Last modified:October 1, 2003 - v1
Checksum:i7F642F7459F8E8C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX548175 Genomic DNA. Translation: CAE20372.1.
RefSeqiWP_011129576.1. NC_005071.1.

Genome annotation databases

EnsemblBacteriaiCAE20372; CAE20372; PMT_0197.
KEGGipmt:PMT0197.
PATRICi23008113. VBIProMar135351_0217.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX548175 Genomic DNA. Translation: CAE20372.1.
RefSeqiWP_011129576.1. NC_005071.1.

3D structure databases

ProteinModelPortaliQ7V8X5.
SMRiQ7V8X5. Positions 2-185.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi74547.PMT0197.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE20372; CAE20372; PMT_0197.
KEGGipmt:PMT0197.
PATRICi23008113. VBIProMar135351_0217.

Phylogenomic databases

eggNOGiCOG1791.
KOiK08967.
OMAiWFDMGSE.
OrthoDBiEOG6ZPT06.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00878.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_01682. Salvage_MtnD.
InterProiIPR023956. Acireductn_d0ase.
IPR004313. Acireductn_dOase_family.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR23418. PTHR23418. 1 hit.
PfamiPF03079. ARD. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MIT 9313.

Entry informationi

Entry nameiMTND_PROMM
AccessioniPrimary (citable) accession number: Q7V8X5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: October 1, 2003
Last modified: July 22, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.