Q7V8B5 (GLGB_PROMM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE

Gene names

Name:	glgB
Ordered Locus Names:	PMT_0444

Organism

Prochlorococcus marinus (strain MIT 9313) [Complete proteome] [HAMAP]

Taxonomic identifier

74547 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Prochlorales › Prochlorococcaceae › Prochlorococcus › Prochlorococcus marinus

Protein attributes

Sequence length	756 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-HAMAP
Molecular_function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: UniProtKB-HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 756	756	1,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685		PRO_0000188728
Sites
	Active site	431	1	Nucleophile By similarity
	Active site	484	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7V8B5 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: ECBB3AB60700C4AA	FASTA	756	87,745
	10 20 30 40 50 60 MTTSVALDWV VQDGQRLAEC RHDDPFSLLG PQSHEGQWIV RIWMPEASQV ELLCDGRTTA 70 80 90 100 110 120 MTTPNHSWIF EAALNQNPGR TYQVRVKRAG IVHEQHDPWA FHDEWMGEMD RHLFAEGNHH 130 140 150 160 170 180 HIWQRMGAHL MEREGVEGVM FCLWAPHACS VAVLGELNGW DGRHHPMQRR QGGLWELFIP 190 200 210 220 230 240 GFKEGTLYKY EIRTQEGHCY QKADPYGFQH EVRPATSSVV ARLDRYQWQD EQWMRQRDSR 250 260 270 280 290 300 NALDQPISVY EMHLGSWIHA ATDEPYIELD GTPRAPVPAA DMKPGARLLT YPELADQLIP 310 320 330 340 350 360 YVKDRGFTHI ELMPISEHPF DGSWGYQVTG WYAPTSRFGS PDEFRAFVDR CHAEGLGVII 370 380 390 400 410 420 DWVPGHFPKD GHGLAFFDGT HLYEHSDPRI GEHKEWGTLI FNYSRNEVRN FLVANLVYWF 430 440 450 460 470 480 EQFHIDGIRV DAVASMLYRD YLRPDGEWIA NEDGGRENTE AVRFLQQANH VLFQHFPGAL 490 500 510 520 530 540 SIAEESTTWP MVTQPTDIGG LGFNLKWNMG WMHDMLDYFE LDPWFRQFHQ NNITFSIWYA 550 560 570 580 590 600 YTENFMLALS HDEVVHGKSN LLHKMPGDDW QKFANVRALL AYMWTHPGKK TIFMGMEFGQ 610 620 630 640 650 660 RSEWNVWGDL QWELLKHDPH KGLQRLVDDL NTFYKGEPAL WKDDFDQYGF QWIDCNDNRH 670 680 690 700 710 720 SVISFMRRES SGGTWLVVVA NFTPQSHSNY RIGVPMSGYY QEVFNTDSSC YGGRNLGNMG 730 740 750 GKNTDEFNIH GYEQSLELCL PALSVLVFRH DPKRSL

References

[1]

"Genome divergence in two Prochlorococcus ecotypes reflects oceanic niche differentiation." Rocap G., Larimer F.W., Lamerdin J.E., Malfatti S., Chain P., Ahlgren N.A., Arellano A., Coleman M., Hauser L., Hess W.R., Johnson Z.I., Land M.L., Lindell D., Post A.F., Regala W., Shah M., Shaw S.L., Steglich C. , Sullivan M.B., Ting C.S., Tolonen A., Webb E.A., Zinser E.R., Chisholm S.W. Nature 424:1042-1047(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MIT 9313.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX548175 Genomic DNA. Translation: CAE20619.1.
RefSeq	NP_894277.1. NC_005071.1.
3D structure databases
ProteinModelPortal	Q7V8B5.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
STRING	74547.PMT0444.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAE20619; CAE20619; PMT_0444.
GeneID	1729755.
KEGG	pmt:PMT0444.
PATRIC	23008755. VBIProMar135351_0535.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
KO	K00700.
OMA	AKLLFMG.
OrthoDB	EOG6JX7GT.
Enzyme and pathway databases
UniPathway	UPA00164.
Family and domain databases
Gene3D	2.60.40.10. 2 hits. 2.60.40.1180. 1 hit. 3.20.20.80. 1 hit.
HAMAP	MF_00685. GlgB.
InterPro	IPR006048. A-amylase_b_C. IPR006407. GlgB. IPR015902. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
PANTHER	PTHR10357. PTHR10357. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. SSF51445. 1 hit. SSF81296. SSF81296. 2 hits.
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_PROMM

Accession

Primary (citable) accession number: Q7V8B5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	October 1, 2003
Last modified:	June 11, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program