Q7V6G8 (GLO2_PROMM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	PMT_1194

Organism

Prochlorococcus marinus (strain MIT 9313) [Complete proteome] [HAMAP]

Taxonomic identifier

74547 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_0000309680
Sites
	Metal binding	66	1	Zinc 1 By similarity
	Metal binding	68	1	Zinc 1 By similarity
	Metal binding	70	1	Zinc 2 By similarity
	Metal binding	71	1	Zinc 2 By similarity
	Metal binding	125	1	Zinc 1 By similarity
	Metal binding	150	1	Zinc 1 By similarity
	Metal binding	150	1	Zinc 2 By similarity
	Metal binding	188	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7V6G8 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: A384BD33702814B5	FASTA	260	28,990
	10 20 30 40 50 60 MEATSTAATS DKQRSSIHAL PVLQDNIIWI WIKGNQAVVV DPAIAEPVKT WLRTRKLSLA 70 80 90 100 110 120 AVLQTHHHAD HIGGTLELLR DWPNAAVVAA AEDRDRIPFQ TISVRDRDKV SLLNSSVEVL 130 140 150 160 170 180 AVAGHTRAHI AYYLPTNKED LEDPAVFCGD TLFGAGCGRL FEGTAEDMFK ALQRLSCLPA 190 200 210 220 230 240 KTRVYCAHEY TEANLRWASA LHPEDVAISE RLADVSSRRQ RGALSLPSSI SEEQRTNLFV 250 260 RAQSSKELAE LRHHKDQWRN

References

[1]

"Genome divergence in two Prochlorococcus ecotypes reflects oceanic niche differentiation." Rocap G., Larimer F.W., Lamerdin J.E., Malfatti S., Chain P., Ahlgren N.A., Arellano A., Coleman M., Hauser L., Hess W.R., Johnson Z.I., Land M.L., Lindell D., Post A.F., Regala W., Shah M., Shaw S.L., Steglich C. , Sullivan M.B., Ting C.S., Tolonen A., Webb E.A., Zinser E.R., Chisholm S.W. Nature 424:1042-1047(2003) [PubMed: 12917642] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MIT 9313.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX548175 Genomic DNA. Translation: CAE21369.1.
RefSeq	NP_895024.1. NC_005071.1.
3D structure databases
HSSP	HSSP built from PDB template 1QH5 based on UniProtKB Q16775.
ProteinModelPortal	Q7V6G8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7V6G8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1729141.
GenomeReviews	Gene locus PMT_1194 in contig BX548175_GR.
KEGG	pmt:PMT1194.
NMPDR	fig|74547.1.peg.1191.
PATRIC	23010793. VBIProMar135351_1545.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HBG753931.
OMA	ITHHHRD.
PhylomeDB	Q7V6G8.
ProtClustDB	CLSK647185.
Enzyme and pathway databases
BioCyc	PMAR74547:PMT1194-MONOMER.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_PROMM

Accession

Primary (citable) accession number: Q7V6G8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program