Reviewed, UniProtKB/Swiss-Prot Q7V654 (UCRI_PROMM)

Names and origin

Protein names

Recommended name:
    Cytochrome b6-f complex iron-sulfur subunit
    EC=1.10.99.1
Alternative name(s):
    Rieske iron-sulfur protein
    Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
      Short name=ISP
      Short name=RISP

Gene names

Name:	petC
Ordered Locus Names:	PMT_1322

Organism

Prochlorococcus marinus (strain MIT 9313) [Complete proteome] [HAMAP]

Taxonomic identifier

74547 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions By similarity.
Catalytic activity	Plastoquinol-1 + 2 oxidized plastocyanin = plastoquinone + 2 reduced plastocyanin. HAMAP MF_01335
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer By similarity.
Subcellular location	Cellular thylakoid membrane; Single-pass membrane protein By similarity. Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer By similarity.
Miscellaneous	The Rieske iron-sulfur protein is a high potential 2Fe-2S protein. HAMAP MF_01335
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Membrane Thylakoid
Domain	Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW plastoquinol-plastocyanin reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 178	178	Cytochrome b6-f complex iron-sulfur subunit HAMAP MF_01335		PRO_0000127778
Regions
	Transmembrane	20 – 42	23	Potential
	Domain	68 – 161	94	Rieske
Sites
	Metal binding	107	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	109	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	125	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	128	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	112 ↔ 127		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7V654-1 [UniParc]. Last modified July 5, 2005. Version 2. Checksum: 94808A7DC07F0D9F	FASTA	178	18,861
	10 20 30 40 50 60 MTQMTSSDVP GMGRRQFMNL LTFGSVTGVA LGSLYPVVKY FIPPRASGSG GGTSAKDELG 70 80 90 100 110 120 NSVTASGWLA NHSDGDRSLV QGLKGDPTYL IVEGDDAIGS YGINAICTHL GCVVPWNSGV 130 140 150 160 170 NKFVCPCHGS QYNSTGKVVR GPAPLSLALA NIAVENDNVF VSQWTETDFR TGEKPWWT

References

[1]

"Genome divergence in two Prochlorococcus ecotypes reflects oceanic niche differentiation." Rocap G., Larimer F.W., Lamerdin J.E., Malfatti S., Chain P., Ahlgren N.A., Arellano A., Coleman M., Hauser L., Hess W.R., Johnson Z.I., Land M.L., Lindell D., Post A.F., Regala W., Shah M., Shaw S.L., Steglich C. , Sullivan M.B., Ting C.S., Tolonen A., Webb E.A., Zinser E.R., Chisholm S.W. Nature 424:1042-1047(2003) [PubMed: 12917642] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX548175 Genomic DNA. Translation: CAE21497.1. Different initiation.
RefSeq	NP_895149.1.
3D structure databases
HSSP	HSSP built from PDB template 1VF5 based on UniProtKB P83794.
SMR	Q7V654. Positions 12-177.
ModBase	Search...
Genome annotation databases
GeneID	1729248.
GenomeReviews	Gene locus PMT_1322 in contig BX548175_GR.
KEGG	pmt:PMT1322.
NMPDR	fig|74547.1.peg.1316.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7V654.
Enzyme and pathway databases
BioCyc	PMAR74547:PMT1322-MON.
Family and domain databases
HAMAP	MF_01335. [Tree]
InterPro	IPR014909. Cyt_b6-f_cplx_Fe-S_su. IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF08802. CytB6-F_Fe-S. 1 hit. PF00355. Rieske. 1 hit. [Graphical view]
PROSITE	PS51296. RIESKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI_PROMM

Accession

Primary (citable) accession number: Q7V654

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	July 5, 2005
Last modified:	June 16, 2009
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)