Reviewed, UniProtKB/Swiss-Prot Q7V5R2 (FUMC_PROMM)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class II
      Short name=Fumarase C
    EC=4.2.1.2

Gene names

Name:	fumC
Ordered Locus Names:	PMT_1485

Organism

Prochlorococcus marinus (strain MIT 9313) [Complete proteome] [HAMAP]

Taxonomic identifier

74547 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle. HAMAP MF_00743
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161297
Regions
	Region	131 – 134	4	B site By similarity
	Region	141 – 143	3	Substrate binding By similarity
Sites
	Binding site	102	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7V5R2-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 395908D8DD84B12D	FASTA	466	50,233
	10 20 30 40 50 60 MMADLMRIEH DSMGTIEVPA GVLWGAQTQR SLLNFAISTD RMPVELIHAL ALIKQAAASV 70 80 90 100 110 120 NCRLGVLDEV QRDQIIKAAS AVASGLHDDQ FPLRVWQTGS GTHTNMNVNE VISNLASQAN 130 140 150 160 170 180 DEPLGSHRPV HPNDHVNRSQ STNDAFPTAI HIAAVQGITN NLLPELEQLI AAFARKSDAW 190 200 210 220 230 240 SDIIKIGRTH LQDAVPLTLG QEASAWRDQI ASAHSRIQSS LIELYPLPLG GTAVGTGLNA 250 260 270 280 290 300 PARFGQETAA QLASITGLPF SSAKNKFAVM ASHDGLVNAM AQLRMLAVAL FKISNDLRLL 310 320 330 340 350 360 ACGPRAGLAE LHLPENEPGS SIMPGKVNPS QCEAMAMVCL QVIGLDSAVT MAGGSGHLQM 370 380 390 400 410 420 NVYKPLIGFN LLHSIELLHD ACRKYRLAMV QGIEPNRIKI QHDLEQSLML VTALAPEIGY 430 440 450 460 DKASEIAHLA HEKGFSLREA ALKLGYVSKE DFDRIVNPAL MTSARL

References

[1]

"Genome divergence in two Prochlorococcus ecotypes reflects oceanic niche differentiation." Rocap G., Larimer F.W., Lamerdin J.E., Malfatti S., Chain P., Ahlgren N.A., Arellano A., Coleman M., Hauser L., Hess W.R., Johnson Z.I., Land M.L., Lindell D., Post A.F., Regala W., Shah M., Shaw S.L., Steglich C. , Sullivan M.B., Ting C.S., Tolonen A., Webb E.A., Zinser E.R., Chisholm S.W. Nature 424:1042-1047(2003) [PubMed: 12917642] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX548175 Genomic DNA. Translation: CAE21660.1.
RefSeq	NP_895312.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q7V5R2.
Genome annotation databases
GeneID	1728513.
GenomeReviews	Gene locus PMT_1485 in contig BX548175_GR.
KEGG	pmt:PMT1485.
NMPDR	fig|74547.1.peg.1479.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7V5R2.
OMA	GSQGHFE.
Enzyme and pathway databases
BioCyc	PMAR74547:PMT1485-MON.
Family and domain databases
HAMAP	MF_00743. [Tree]
InterPro	IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00149. FUMRATELYASE.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_PROMM

Accession

Primary (citable) accession number: Q7V5R2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)