ID   ACSF_PROMM              Reviewed;         349 AA.
AC   Q7V3Y9;
DT   16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   16-JUN-2009, entry version 37.
DE   RecName: Full=Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase;
DE            Short=Mg-protoporphyrin IX monomethyl ester oxidative cyclase;
DE            EC=1.14.13.81;
GN   Name=acsF; OrderedLocusNames=PMT_2196;
OS   Prochlorococcus marinus (strain MIT 9313).
OC   Bacteria; Cyanobacteria; Prochlorophytes; Prochlorococcaceae;
OC   Prochlorococcus.
OX   NCBI_TaxID=74547;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=22825698; PubMed=12917642; DOI=10.1038/nature01947;
RA   Rocap G., Larimer F.W., Lamerdin J.E., Malfatti S., Chain P.,
RA   Ahlgren N.A., Arellano A., Coleman M., Hauser L., Hess W.R.,
RA   Johnson Z.I., Land M.L., Lindell D., Post A.F., Regala W., Shah M.,
RA   Shaw S.L., Steglich C., Sullivan M.B., Ting C.S., Tolonen A.,
RA   Webb E.A., Zinser E.R., Chisholm S.W.;
RT   "Genome divergence in two Prochlorococcus ecotypes reflects oceanic
RT   niche differentiation.";
RL   Nature 424:1042-1047(2003).
CC   -!- FUNCTION: Catalyzes the formation of the isocyclic ring in
CC       chlorophyll biosynthesis. Mediates the cyclase reaction, which
CC       results in the formation of divinylprotochlorophyllide (Pchlide)
CC       characteristic of all chlorophylls from magnesium-protoporphyrin
CC       IX 13-monomethyl ester (MgPMME) (By similarity).
CC   -!- CATALYTIC ACTIVITY: Magnesium-protoporphyrin IX 13-monomethyl
CC       ester + NADPH + O(2) = 13(1)-hydroxy-magnesium-protoporphyrin IX
CC       13-monomethyl ester + NADP(+) + H(2)O.
CC   -!- CATALYTIC ACTIVITY: 13(1)-hydroxy-magnesium-protoporphyrin IX 13-
CC       monomethyl ester + NADPH + O(2) = 13(1)-oxo-magnesium-
CC       protoporphyrin IX 13-monomethyl ester + NADP(+) + 2 H(2)O.
CC   -!- CATALYTIC ACTIVITY: 13(1)-oxo-magnesium-protoporphyrin IX 13-
CC       monomethyl ester + NADPH + O(2) = divinylprotochlorophyllide +
CC       NADP(+) + 2 H(2)O.
CC   -!- COFACTOR: Iron (By similarity).
CC   -!- PATHWAY: Porphyrin biosynthesis; chlorophyll biosynthesis (light-
CC       independent).
CC   -!- SIMILARITY: Belongs to the acsF family.
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DR   EMBL; BX548175; CAE22370.1; -; Genomic_DNA.
DR   RefSeq; NP_896020.1; -.
DR   GeneID; 1728466; -.
DR   GenomeReviews; BX548175_GR; PMT_2196.
DR   KEGG; pmt:PMT2196; -.
DR   NMPDR; fig|74547.1.peg.2187; -.
DR   HOGENOM; Q7V3Y9; -.
DR   OMA; Q7V3Y9; SMSDFGL.
DR   BioCyc; PMAR74547:PMT2196-MON; -.
DR   GO; GO:0005506; F:iron ion binding; IEA:HAMAP.
DR   GO; GO:0048529; F:magnesium-protoporphyrin IX monomethyl este...; IEA:HAMAP.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0015979; P:photosynthesis; IEA:HAMAP.
DR   HAMAP; MF_01840; -; 1.
DR   InterPro; IPR008434; AcsF.
DR   InterPro; IPR003251; Rubrerythrin.
DR   Pfam; PF02915; Rubrerythrin; 1.
DR   TIGRFAMs; TIGR02029; AcsF; 1.
PE   3: Inferred from homology;
KW   Chlorophyll biosynthesis; Complete proteome; Iron; Metal-binding;
KW   NADP; Oxidoreductase; Photosynthesis.
FT   CHAIN         1    349       Magnesium-protoporphyrin IX monomethyl
FT                                ester [oxidative] cyclase.
FT                                /FTId=PRO_0000217531.
SQ   SEQUENCE   349 AA;  40442 MW;  223C1FA2E192E8E9 CRC64;
     MTATTATAPT MRGGGRNELP PHLDDNLLTP RFYTTEFDKA AKTDLDIARK DFEAMFKEME
     ADYNLKHFDR KASLERLSEL SPEDKAIYES YLVRSVVSEF SGFLLFKEIS NRFKKAGRQE
     LGQFFTFLAR DEARHAGFLG RALKAEGINV DLPNLGNKRA ATFFPLSWVL YSLYLSEKIG
     YWRYILINRH LNDNPEKVCA PLFDFFEPWC QDENRHGDCI NLMMRCWPGM TKGFRGKLLS
     RFFLWSVFLT HTLTVCERGD FYGLLGIDPV LFDEEVIIQT NNTSRNAFPW VYNFDDGKFL
     EMRVQILKAF RNWRESSGLA KPVALSKFVS LILRQFALPM QKTNAVRYG
//