Reviewed, UniProtKB/Swiss-Prot Q7V3Y9 (ACSF_PROMM)

Names and origin

Protein names

Recommended name:
    Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase
      Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase
    EC=1.14.13.81

Gene names

Name:	acsF
Ordered Locus Names:	PMT2196

Organism

Prochlorococcus marinus (strain MIT 9313) [Complete proteome] [HAMAP]

Taxonomic identifier

74547 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity.
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + H(2)O. 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + 2 H(2)O. 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = divinylprotochlorophyllide + NADP(+) + 2 H(2)O.
Cofactor	Iron By similarity.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis (light-independent).
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: HAMAP magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase		PRO_0000217531

Sequences

	Sequence		Length	Mass (Da)
	Q7V3Y9-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 223C1FA2E192E8E9	FASTA	349	40,442
	10 20 30 40 50 60 MTATTATAPT MRGGGRNELP PHLDDNLLTP RFYTTEFDKA AKTDLDIARK DFEAMFKEME 70 80 90 100 110 120 ADYNLKHFDR KASLERLSEL SPEDKAIYES YLVRSVVSEF SGFLLFKEIS NRFKKAGRQE 130 140 150 160 170 180 LGQFFTFLAR DEARHAGFLG RALKAEGINV DLPNLGNKRA ATFFPLSWVL YSLYLSEKIG 190 200 210 220 230 240 YWRYILINRH LNDNPEKVCA PLFDFFEPWC QDENRHGDCI NLMMRCWPGM TKGFRGKLLS 250 260 270 280 290 300 RFFLWSVFLT HTLTVCERGD FYGLLGIDPV LFDEEVIIQT NNTSRNAFPW VYNFDDGKFL 310 320 330 340 EMRVQILKAF RNWRESSGLA KPVALSKFVS LILRQFALPM QKTNAVRYG

References

[1]

"Genome divergence in two Prochlorococcus ecotypes reflects oceanic niche differentiation." Rocap G., Larimer F.W., Lamerdin J.E., Malfatti S., Chain P., Ahlgren N.A., Arellano A., Coleman M., Hauser L., Hess W.R., Johnson Z.I., Land M.L., Lindell D., Post A.F., Regala W., Shah M., Shaw S.L., Steglich C. , Sullivan M.B., Ting C.S., Tolonen A., Webb E.A., Zinser E.R., Chisholm S.W. Nature 424:1042-1047(2003) [PubMed: 12917642] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX572101 Genomic DNA. Translation: CAE22370.1.
RefSeq	NP_896020.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1728466.
GenomeReviews	Gene locus PMT2196 in contig BX548175_GR.
KEGG	pmt:PMT2196.
NMPDR	fig|74547.1.peg.2187.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7V3Y9.
Enzyme and pathway databases
BioCyc	PMAR74547:PMT2196-MON.
Family and domain databases
HAMAP	MF_01840. [Tree]
InterPro	IPR008434. AcsF. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACSF_PROMM

Accession

Primary (citable) accession number: Q7V3Y9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	October 1, 2003
Last modified:	November 25, 2008
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)