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Q7V352

- SYI_PROMP

UniProt

Q7V352 - SYI_PROMP

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Prochlorococcus marinus subsp. pastoris (strain CCMP1986 / MED4)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei582 – 5821Aminoacyl-adenylateUniRule annotation
Binding sitei626 – 6261ATPUniRule annotation
Metal bindingi936 – 9361ZincUniRule annotation
Metal bindingi939 – 9391ZincUniRule annotation
Metal bindingi956 – 9561ZincUniRule annotation
Metal bindingi959 – 9591ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMAR59919:GJMQ-245-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PMM0238
OrganismiProchlorococcus marinus subsp. pastoris (strain CCMP1986 / MED4)
Taxonomic identifieri59919 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000001026: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 965965Isoleucine--tRNA ligasePRO_0000098442Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi59919.PMM0238.

Structurei

3D structure databases

ProteinModelPortaliQ7V352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi68 – 7811"HIGH" regionAdd
BLAST
Motifi623 – 6275"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiEVMDEIT.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7V352-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKSKHNKEKK SGFSYKETLN LLKTDFSMRA NSVIREPEIH EFWSRNKIDL
60 70 80 90 100
ELGSTNSGKN FTLHDGPPYA NGSLHMGHAL NKVLKDIINK YKTLQGFKVH
110 120 130 140 150
FIPGWDCHGL PIELKVLQSL KSNERRNLDS LGLRKKATDY AKIQINNQLE
160 170 180 190 200
GFKRWGIWGD WDNPYLTLKK NYESAQIGVF GKMFLNGYIY RGLKPVHWSP
210 220 230 240 250
SSRTALAEAE LEYPEEHFSK SIYVSLNITK LSDAVLLKLE EKDLRNKLLS
260 270 280 290 300
SLNKLFIAIW TTTPWTIPGN EAVAINPRIN YVFASDQNGN IYLFARDLIS
310 320 330 340 350
EICEKLDREF NVLLDVKGSL LEGLEYKHPT KNKFCNIVIG GDYITTESGT
360 370 380 390 400
GIVHTAPGHG MDDFNVGQKY QLPITCIVDE KGNLNKHAEK FCGLNVLKDA
410 420 430 440 450
NDLIIEDLEK NNLLLLKENY KHRYPYDWRT KKPTIFRATE QWFASVEGFR
460 470 480 490 500
SSALKAIEDV EWMPKTGKKR IYSMVVGRGD WCISRQRSWG VPIPVFYEKE
510 520 530 540 550
GKGVLINTET INHIKSLFEE YGADVWWEWD EKKLLPEKYR NESDRWEKGL
560 570 580 590 600
DTMDVWFDSG SSWAAVCEQR EELQYPADLY LEGSDQHRGW FQSSLLTSVA
610 620 630 640 650
VNNKPPYKTV LTHGFALDEN GRKMSKSLGN VVDPNIIING GPNQKIQPAY
660 670 680 690 700
GADVLRLWVS SVDYSVDVPI GSNILKQLSD VYRKVRNTAR YLLGNIHDYD
710 720 730 740 750
PALEEIDIDQ LPLLDQWMLN RLVEVSDEVT IAYENYEFSK FFQILQSFCV
760 770 780 790 800
VDLSNFYLDI AKDRLYVSAK TQFRRRSCQF VMSRIVENLA VIISPVLCHM
810 820 830 840 850
AEDIWQNIPY STIEKSVFER RWPKFPESWL NPELNEHISN LRTLRVEINK
860 870 880 890 900
AIEGCRTQQM IGAALETEVN YLPENEFFKN SLSWLDKFGN KEVDLYRDWL
910 920 930 940 950
IVSDFNIVNN LSKDCLSIDN NELGKIQILK AKGLKCDRCW HYQNITVKGI
960
ENTKLCERCA NIINL
Length:965
Mass (Da):111,413
Last modified:October 1, 2003 - v1
Checksum:i06D4C8BF586957A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX548174 Genomic DNA. Translation: CAE18697.1.
RefSeqiNP_892357.1. NC_005072.1.
WP_011131876.1. NC_005072.1.

Genome annotation databases

EnsemblBacteriaiCAE18697; CAE18697; PMM0238.
GeneIDi1726535.
KEGGipmm:PMM0238.
PATRICi23031124. VBIProMar68066_0245.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX548174 Genomic DNA. Translation: CAE18697.1 .
RefSeqi NP_892357.1. NC_005072.1.
WP_011131876.1. NC_005072.1.

3D structure databases

ProteinModelPortali Q7V352.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 59919.PMM0238.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE18697 ; CAE18697 ; PMM0238 .
GeneIDi 1726535.
KEGGi pmm:PMM0238.
PATRICi 23031124. VBIProMar68066_0245.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi EVMDEIT.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PMAR59919:GJMQ-245-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CCMP1986 / MED4.

Entry informationi

Entry nameiSYI_PROMP
AccessioniPrimary (citable) accession number: Q7V352
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2003
Last modified: November 26, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3