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Q7V352

- SYI_PROMP

UniProt

Q7V352 - SYI_PROMP

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Protein
Isoleucine--tRNA ligase
Gene
ileS, PMM0238
Organism
Prochlorococcus marinus subsp. pastoris (strain CCMP1986 / MED4)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei582 – 5821Aminoacyl-adenylate By similarity
Binding sitei626 – 6261ATP By similarity
Metal bindingi936 – 9361Zinc By similarity
Metal bindingi939 – 9391Zinc By similarity
Metal bindingi956 – 9561Zinc By similarity
Metal bindingi959 – 9591Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMAR59919:GJMQ-245-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:PMM0238
OrganismiProchlorococcus marinus subsp. pastoris (strain CCMP1986 / MED4)
Taxonomic identifieri59919 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000001026: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 965965Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098442Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi59919.PMM0238.

Structurei

3D structure databases

ProteinModelPortaliQ7V352.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi68 – 7811"HIGH" regionUniRule annotation
Add
BLAST
Motifi623 – 6275"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiEVMDEIT.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7V352-1 [UniParc]FASTAAdd to Basket

« Hide

MKSKHNKEKK SGFSYKETLN LLKTDFSMRA NSVIREPEIH EFWSRNKIDL    50
ELGSTNSGKN FTLHDGPPYA NGSLHMGHAL NKVLKDIINK YKTLQGFKVH 100
FIPGWDCHGL PIELKVLQSL KSNERRNLDS LGLRKKATDY AKIQINNQLE 150
GFKRWGIWGD WDNPYLTLKK NYESAQIGVF GKMFLNGYIY RGLKPVHWSP 200
SSRTALAEAE LEYPEEHFSK SIYVSLNITK LSDAVLLKLE EKDLRNKLLS 250
SLNKLFIAIW TTTPWTIPGN EAVAINPRIN YVFASDQNGN IYLFARDLIS 300
EICEKLDREF NVLLDVKGSL LEGLEYKHPT KNKFCNIVIG GDYITTESGT 350
GIVHTAPGHG MDDFNVGQKY QLPITCIVDE KGNLNKHAEK FCGLNVLKDA 400
NDLIIEDLEK NNLLLLKENY KHRYPYDWRT KKPTIFRATE QWFASVEGFR 450
SSALKAIEDV EWMPKTGKKR IYSMVVGRGD WCISRQRSWG VPIPVFYEKE 500
GKGVLINTET INHIKSLFEE YGADVWWEWD EKKLLPEKYR NESDRWEKGL 550
DTMDVWFDSG SSWAAVCEQR EELQYPADLY LEGSDQHRGW FQSSLLTSVA 600
VNNKPPYKTV LTHGFALDEN GRKMSKSLGN VVDPNIIING GPNQKIQPAY 650
GADVLRLWVS SVDYSVDVPI GSNILKQLSD VYRKVRNTAR YLLGNIHDYD 700
PALEEIDIDQ LPLLDQWMLN RLVEVSDEVT IAYENYEFSK FFQILQSFCV 750
VDLSNFYLDI AKDRLYVSAK TQFRRRSCQF VMSRIVENLA VIISPVLCHM 800
AEDIWQNIPY STIEKSVFER RWPKFPESWL NPELNEHISN LRTLRVEINK 850
AIEGCRTQQM IGAALETEVN YLPENEFFKN SLSWLDKFGN KEVDLYRDWL 900
IVSDFNIVNN LSKDCLSIDN NELGKIQILK AKGLKCDRCW HYQNITVKGI 950
ENTKLCERCA NIINL 965
Length:965
Mass (Da):111,413
Last modified:October 1, 2003 - v1
Checksum:i06D4C8BF586957A7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX548174 Genomic DNA. Translation: CAE18697.1.
RefSeqiNP_892357.1. NC_005072.1.
WP_011131876.1. NC_005072.1.

Genome annotation databases

EnsemblBacteriaiCAE18697; CAE18697; PMM0238.
GeneIDi1726535.
KEGGipmm:PMM0238.
PATRICi23031124. VBIProMar68066_0245.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX548174 Genomic DNA. Translation: CAE18697.1 .
RefSeqi NP_892357.1. NC_005072.1.
WP_011131876.1. NC_005072.1.

3D structure databases

ProteinModelPortali Q7V352.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 59919.PMM0238.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE18697 ; CAE18697 ; PMM0238 .
GeneIDi 1726535.
KEGGi pmm:PMM0238.
PATRICi 23031124. VBIProMar68066_0245.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi EVMDEIT.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PMAR59919:GJMQ-245-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CCMP1986 / MED4.

Entry informationi

Entry nameiSYI_PROMP
AccessioniPrimary (citable) accession number: Q7V352
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2003
Last modified: September 3, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi