Q7V329 (ISPH_PROMP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Synonyms:	lytB
Ordered Locus Names:	PMM0264

Organism

Prochlorococcus marinus subsp. pastoris (strain CCMP1986 / MED4) [Complete proteome] [HAMAP]

Taxonomic identifier

59919 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_0000128856

Sequences

	Sequence		Length	Mass (Da)
	Q7V329 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 8EA1FC0CCC78B854	FASTA	398	45,202
	10 20 30 40 50 60 MDTQAFRRSL HHSDRYNRRG FDSPTKRAQA LEEAYQSDLI TSIRNNSFTY KKGRLNIKLA 70 80 90 100 110 120 QAFGFCWGVE RAVAMAYETR RHYPDENIWI TNEIIHNPSV NDHLRKMNVK FISAKNGIKD 130 140 150 160 170 180 FSLVSDGDVV ILPAFGATVQ EMKLLHEKGC HIIDTTCPWV SKVWHTVEKH KKHTFTSIIH 190 200 210 220 230 240 GKFKHEETLA TSSFADKYLV VLNLEEANYV SEYILGRGNK NHFLNKFAQA FSNGFDPDKD 250 260 270 280 290 300 LDRVGVANQT TMLKSETEEI GKVFEKTMLH KFGPEKLNSH FLAFNTICDA TEERQDAMFS 310 320 330 340 350 360 LVDEDLDILV VIGGYNSSNT THLQEIAINK NISSFHIDTP ERISVKENSI LHKPLKSEMV 370 380 390 LKKNFIPDGE IKVGITSGAS TPDKIVADVI EKLIAITK

References

[1]

"Genome divergence in two Prochlorococcus ecotypes reflects oceanic niche differentiation." Rocap G., Larimer F.W., Lamerdin J.E., Malfatti S., Chain P., Ahlgren N.A., Arellano A., Coleman M., Hauser L., Hess W.R., Johnson Z.I., Land M.L., Lindell D., Post A.F., Regala W., Shah M., Shaw S.L., Steglich C. , Sullivan M.B., Ting C.S., Tolonen A., Webb E.A., Zinser E.R., Chisholm S.W. Nature 424:1042-1047(2003) [PubMed: 12917642] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CCMP1986 / MED4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX548174 Genomic DNA. Translation: CAE18723.1.
RefSeq	NP_892383.1. NC_005072.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q7V329.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1726268.
GenomeReviews	Gene locus PMM0264 in contig BX548174_GR.
KEGG	pmm:PMM0264.
NMPDR	fig|59919.1.peg.262.
PATRIC	23031180. VBIProMar68066_0272.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0761.
HOGENOM	HBG286221.
OMA	VQEMQLL.
PhylomeDB	Q7V329.
ProtClustDB	PRK13371.
Enzyme and pathway databases
BioCyc	PMAR167540:PMM0264-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_PROMP

Accession

Primary (citable) accession number: Q7V329

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program