Q7V2K3 (SYE_PROMP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	PMM0473

Organism

Prochlorococcus marinus subsp. pastoris (strain CCMP1986 / MED4) [Complete proteome] [HAMAP]

Taxonomic identifier

59919 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000119626
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00022_B
	Motif	248 – 252	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	251	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7V2K3 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: E77F0448B7960EC3	FASTA	478	55,384
	10 20 30 40 50 60 MEKHLRLAPS PTGLLHIGTA RTALFNWLYA RKINGKFLIR IEDTDIVRSK SEYTTNILNG 70 80 90 100 110 120 LNWLGLNWDE EPINQSKRVS VHKNYIKRLL ESGAAYRCFT SESEILDLRE KQKKSGLPPK 130 140 150 160 170 180 HDNRHRNLTR KEIKEFISQG KSSVIRFKID EETEIKWEDQ IRGEIKWQGK DLGGDLVLSR 190 200 210 220 230 240 RALGDEIGNP LYNLAVVVDD NFMNITHVVR GEDHISNTAK QILIYKALNF KLPIFAHTPL 250 260 270 280 290 300 ILNSEGKKLS KRDSVTSIDE FKEMGYLPEA LANYMAFLGW SIKSPESEIL SLSEISKVFN 310 320 330 340 350 360 LSDVNKAGAK FNWEKLNWIN SQYIKKMELT QLCKFIKKYW EEMGWESPSS EWDLKLTNLI 370 380 390 400 410 420 QDSMILLKDA IDQSKPFFIL SQMKKEGEEF LESKKEVKES LKYILFYLKE ANITKVNKDN 430 440 450 460 470 AREIINKIIV NHSIKKGILM KSLRVAFFGC LSGPDLIQSW ELFSENKIDI PLIERCFN

References

[1]

"Genome divergence in two Prochlorococcus ecotypes reflects oceanic niche differentiation." Rocap G., Larimer F.W., Lamerdin J.E., Malfatti S., Chain P., Ahlgren N.A., Arellano A., Coleman M., Hauser L., Hess W.R., Johnson Z.I., Land M.L., Lindell D., Post A.F., Regala W., Shah M., Shaw S.L., Steglich C. , Sullivan M.B., Ting C.S., Tolonen A., Webb E.A., Zinser E.R., Chisholm S.W. Nature 424:1042-1047(2003) [PubMed: 12917642] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CCMP1986 / MED4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX548174 Genomic DNA. Translation: CAE18932.1.
RefSeq	NP_892591.1. NC_005072.1.
3D structure databases
ProteinModelPortal	Q7V2K3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7V2K3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1726650.
GenomeReviews	Gene locus PMM0473 in contig BX548174_GR.
KEGG	pmm:PMM0473.
NMPDR	fig|59919.1.peg.470.
PATRIC	23031710. VBIProMar68066_0527.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	DKETAND.
PhylomeDB	Q7V2K3.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	PMAR167540:PMM0473-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_PROMP

Accession

Primary (citable) accession number: Q7V2K3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program