Reviewed, UniProtKB/Swiss-Prot Q7V1M1 (ACSF_PROMP)

Names and origin

Protein names

Recommended name:
    Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase
      Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase
    EC=1.14.13.81

Gene names

Name:	acsF
Ordered Locus Names:	PMM0844

Organism

Prochlorococcus marinus subsp. pastoris (strain CCMP1378 / MED4) [Complete proteome] [HAMAP]

Taxonomic identifier

59919 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity.
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + H(2)O. 13(1)-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP(+) + 2 H(2)O. 13(1)-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O(2) = divinylprotochlorophyllide + NADP(+) + 2 H(2)O.
Cofactor	Iron By similarity.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis (light-independent).
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: HAMAP magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase		PRO_0000217532

Sequences

	Sequence		Length	Mass (Da)
	Q7V1M1-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: A55456142B4B34BF	FASTA	390	45,980
	10 20 30 40 50 60 MAQQTIESNN KKSVNRGKDI AKDTILTPNF YTTDFEAMEK MDLSINEDEL EAICEEFRKD 70 80 90 100 110 120 YNRHHFVRNK EFEGAADKID AETRELFVDF LEGSCTSEFS GFLLYKELSK RIKDKNPLLA 130 140 150 160 170 180 ECFAHMARDE ARHAGFLNKS MNDFGLQLDL GFLTANKDYT YFAPRAIFYA TYISEKIGYW 190 200 210 220 230 240 RYIAIYRHLE KNPSGKIFPL FNFFENWCQD ENRHGDFFDA LMKAQPRTVK SLSQKIEIFG 250 260 270 280 290 300 YTLKHPIFDY YHRFRYFLNN HPIVSKLWSR FFLLAVFATM YIRDLGTKRN FYGALGLNAR 310 320 330 340 350 360 EYDQFVINKT NETSAKVFPV VLNVYDKSFY KRLDRIVENG TRLSEIDKKE NPNVIKVLSK 370 380 390 LPIFISNGYQ LIRLYLLKPL ESDDFQPSIR

References

[1]

"Genome divergence in two Prochlorococcus ecotypes reflects oceanic niche differentiation." Rocap G., Larimer F.W., Lamerdin J.E., Malfatti S., Chain P., Ahlgren N.A., Arellano A., Coleman M., Hauser L., Hess W.R., Johnson Z.I., Land M.L., Lindell D., Post A.F., Regala W., Shah M., Shaw S.L., Steglich C. , Sullivan M.B., Ting C.S., Tolonen A., Webb E.A., Zinser E.R., Chisholm S.W. Nature 424:1042-1047(2003) [PubMed: 12917642] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX548174 Genomic DNA. Translation: CAE19303.1.
RefSeq	NP_892962.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1727176.
GenomeReviews	Gene locus PMM0844 in contig BX548174_GR.
KEGG	pmm:PMM0844.
NMPDR	fig|59919.1.peg.841.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7V1M1.
Enzyme and pathway databases
BioCyc	PMAR167540:PMM0844-MON.
Family and domain databases
HAMAP	MF_01840. [Tree]
InterPro	IPR008434. AcsF. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACSF_PROMP

Accession

Primary (citable) accession number: Q7V1M1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	October 1, 2003
Last modified:	November 25, 2008
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)