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Q7V1I2 (SYL_PROMP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:PMM0889
OrganismProchlorococcus marinus subsp. pastoris (strain CCMP1986 / MED4) [Complete proteome] [HAMAP]
Taxonomic identifier59919 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus

Protein attributes

Sequence length858 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 858858Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152065

Regions

Motif53 – 6311"HIGH" region HAMAP-Rule MF_00049
Motif622 – 6265"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6251ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7V1I2 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: 8CE816904876088F

FASTA85899,148
        10         20         30         40         50         60 
MITSENTDNR ATNLYKPSDI EGKWQKIWED DNLYNTDEQA SNKEKFYALS MFPYPSGNLH 

        70         80         90        100        110        120 
MGHVRNYVIT DLIARFQRFQ GKVVLHPMGW DAFGLPAENA AIERGINPDK WTKQNIAHMK 

       130        140        150        160        170        180 
SQLKLLGLSV DWDREFATCD ENYYVWTQFL FLELHKAGLV YQKESEVNWD PIDNTVLANE 

       190        200        210        220        230        240 
QVDSEGKSWR SGAIVEKKLL TQWFLKITDY AEELLQDLEK LNEWPERVKI MQENWIGKSI 

       250        260        270        280        290        300 
GTNINFKIKE FKKEKIQVFT TRPDTLFGVT YLAISVNHPL IKKISDNKIL SKLENLKIYL 

       310        320        330        340        350        360 
QESKDKDQKK IGIPTNLIAI NPINSKEIPI LIASYVLDEY GTGAVMGVPA HDERDFEFAK 

       370        380        390        400        410        420 
INSIDIKQVI IKEKDKITSQ LTNAFTDNGF LINSNNFDGL NNSDAKKHIS EHGERNGWAE 

       430        440        450        460        470        480 
NKIQFRLRDW LISRQRYWGC PIPIIKCTNC GSVPVNKKDI PVRLPNEIKI SSNKINSLGS 

       490        500        510        520        530        540 
NQSWINTTCP KCGNLASRET DTMDTFMCSS WYFLRYPSSK SLTKPFEKEK INKWLPVDQY 

       550        560        570        580        590        600 
VGGVEHAILH LLYARFLTKA LRDNNLFDID EPFKRLLTQG MVQSAAYKNS ITGKYISPTD 

       610        620        630        640        650        660 
IKDITNPKDP KDNSKLEVLF EKMSKSKYNG IDPESVIKKY GADTARMFIL FKAPPEKDLE 

       670        680        690        700        710        720 
WGDSDVEGQY RFLCRIWKLF LDYSNNDITH EADKLKKENE SSLLKSINIA IKEISNDIKN 

       730        740        750        760        770        780 
NQFNTAISEL MKFYNSISSN LNYVNKDLRR ESLMKFCILL APFAPHISDE IWHLIGNSKS 

       790        800        810        820        830        840 
VHLEKWPVFD EDALKENSFE LVIQINGKVR DKINVEINIS DDEIKEKTLI RPNVKKWIDN 

       850 
KTIRKIIIVK GRIINIVV 

« Hide

References

[1]"Genome divergence in two Prochlorococcus ecotypes reflects oceanic niche differentiation."
Rocap G., Larimer F.W., Lamerdin J.E., Malfatti S., Chain P., Ahlgren N.A., Arellano A., Coleman M., Hauser L., Hess W.R., Johnson Z.I., Land M.L., Lindell D., Post A.F., Regala W., Shah M., Shaw S.L., Steglich C. expand/collapse author list , Sullivan M.B., Ting C.S., Tolonen A., Webb E.A., Zinser E.R., Chisholm S.W.
Nature 424:1042-1047(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CCMP1986 / MED4.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX548174 Genomic DNA. Translation: CAE19348.1.
RefSeqNP_893007.1. NC_005072.1.

3D structure databases

ProteinModelPortalQ7V1I2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING59919.PMM0889.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAE19348; CAE19348; PMM0889.
GeneID1726569.
KEGGpmm:PMM0889.
PATRIC23032694. VBIProMar68066_1012.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAVHRQSWP.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycPMAR59919:GJMQ-914-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PROMP
AccessionPrimary (citable) accession number: Q7V1I2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: October 1, 2003
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries