Q7V024 (FUMC_PROMP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fumarate hydratase class II
Short name=Fumarase C
EC=4.2.1.2

Gene names

Name:	fumC
Ordered Locus Names:	PMM1466

Organism

Prochlorococcus marinus subsp. pastoris (strain CCMP1986 / MED4) [Complete proteome] [HAMAP]

Taxonomic identifier

59919 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. HAMAP MF_00743
Subunit structure	Homotetramer By similarity. HAMAP MF_00743
Subcellular location	Cytoplasm By similarity HAMAP MF_00743.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity. HAMAP MF_00743
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161298
Regions
	Region	130 – 133	4	B site By similarity
	Region	140 – 142	3	Substrate binding By similarity
Sites
	Binding site	101	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7V024 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: D696F9A39BF50149	FASTA	461	50,624
	10 20 30 40 50 60 MTKNFRFEKD SMGQIKVPSE ALWGAQTQRS IINFSIGEEL IPIELIYSLT VIKRAAAISN 70 80 90 100 110 120 FKLGLINNVK KDLIIEACTE ILDGKHDSQF PLKIWQTGSG TQTNMNINEV ISNIAALKTN 130 140 150 160 170 180 SGLGSHHPIH PNDDVNKSQS TNDTFPAAIQ ISVVTQIIKK LVPSIKELTK VLDSKSNKWK 190 200 210 220 230 240 DLIKIGRTHF QDAVPISLGQ EVSAWSKQLK DAEDALIISL NELCFLPLGG TAVGTGINCP 250 260 270 280 290 300 KDFSKESIKS ISEYTGLFFY KSKNHFSLMA SHDRLAQVMG QIKILASALF KISNDIKILS 310 320 330 340 350 360 SGPRSGIYEL IIPKNEPGSS IMPGKVNPTQ CEALSMVCTQ VMGFEYAVSI ANASGTLQMN 370 380 390 400 410 420 EYKPLIGFNI LTSIKLLNHA ISNFRLKLVE GIQPNPKTIK ANLENSLMLV TALVPKIGYE 430 440 450 460 KAAEIANLAF NESINLKEAT IKLGYLTANE FEDAINTNKM I

References

[1]

"Genome divergence in two Prochlorococcus ecotypes reflects oceanic niche differentiation." Rocap G., Larimer F.W., Lamerdin J.E., Malfatti S., Chain P., Ahlgren N.A., Arellano A., Coleman M., Hauser L., Hess W.R., Johnson Z.I., Land M.L., Lindell D., Post A.F., Regala W., Shah M., Shaw S.L., Steglich C. , Sullivan M.B., Ting C.S., Tolonen A., Webb E.A., Zinser E.R., Chisholm S.W. Nature 424:1042-1047(2003) [PubMed: 12917642] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CCMP1986 / MED4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX548174 Genomic DNA. Translation: CAE19925.1.
RefSeq	NP_893583.1. NC_005072.1.
3D structure databases
ProteinModelPortal	Q7V024.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7V024.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1725618.
GenomeReviews	Gene locus PMM1466 in contig BX548174_GR.
KEGG	pmm:PMM1466.
NMPDR	fig|59919.1.peg.1462.
PATRIC	23034108. VBIProMar68066_1707.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0114.
HOGENOM	HBG284369.
OMA	EALSMVC.
PhylomeDB	Q7V024.
ProtClustDB	PRK00485.
Enzyme and pathway databases
BioCyc	PMAR167540:PMM1466-MONOMER.
Family and domain databases
HAMAP	MF_00743. FumaraseC. [Tree]
InterPro	IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. IPR024083. L-Aspartase-like_N. IPR022761. Lyase1_N. [Graphical view]
Gene3D	G3DSA:1.10.275.10. G3DSA:1.10.275.10. 1 hit.
KO	K01679.
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00149. FUMRATELYASE.
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_PROMP

Accession

Primary (citable) accession number: Q7V024

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program