Q7UQA4 (SYW_RHOBA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan--tRNA ligase
EC=6.1.1.2

Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS

Gene names

Name:	trpS
Ordered Locus Names:	RB6436

Organism

Rhodopirellula baltica (strain SH1) [Reference proteome] [HAMAP]

Taxonomic identifier

243090 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Planctomycetes › Planctomycetia › Planctomycetales › Planctomycetaceae › Rhodopirellula › Rhodopirellula baltica

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00140
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00140.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tryptophanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP tryptophan-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Tryptophan--tRNA ligase HAMAP-Rule MF_00140		PRO_0000136668
Regions
	Motif	9 – 17	9	"HIGH" region HAMAP-Rule MF_00140
	Motif	189 – 193	5	"KMSKS" region HAMAP-Rule MF_00140
Sites
	Binding site	192	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7UQA4 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: F185D2350B7F523C	FASTA	320	35,533
	10 20 30 40 50 60 MRVLSGIQPT GRPHWGNYFG AIRQYIDLQE DNEGFYFIAD LHALTTVREP EVLRENVMNA 70 80 90 100 110 120 ALDLLALGLD PSKANLFVQS DIPEVTELTW LLMTGTPMGL LERCHAFKEK KAKGLTADAG 130 140 150 160 170 180 LFTYPVLMAA DILAYDSQIV PVGVDQVQHI EVCRDLAGSF HHAFGETFVL PKAKTLDVGA 190 200 210 220 230 240 KVPGTDGQKM SKSYNNTLPL FGEVKKIRKQ IMRIVTDSRP MEDPKDPTDD HLFQLYQLFA 250 260 270 280 290 300 GPAEVETMAA KYRAGGFGYG EIKKAVAEVS EEYFAPARAK REELESDLDT VRDILAEGAK 310 320 RAREVAASVV DRARRNCGLR

References

[1]

"Complete genome sequence of the marine planctomycete Pirellula sp. strain 1." Gloeckner F.O., Kube M., Bauer M., Teeling H., Lombardot T., Ludwig W., Gade D., Beck A., Borzym K., Heitmann K., Rabus R., Schlesner H., Amann R., Reinhardt R. Proc. Natl. Acad. Sci. U.S.A. 100:8298-8303(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SH1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX294144 Genomic DNA. Translation: CAD74801.1.
RefSeq	NP_867255.1. NC_005027.1.
3D structure databases
ProteinModelPortal	Q7UQA4.
ModBase	Search...
Protein-protein interaction databases
STRING	243090.RB6436.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD74801; CAD74801; RB6436.
GeneID	1791723.
KEGG	rba:RB6436.
PATRIC	23247675. VBIRhoBal59814_3112.
Phylogenomic databases
eggNOG	COG0180.
HOGENOM	HOG000059939.
KO	K01867.
OMA	SVDKNVE.
ProtClustDB	PRK00927.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00140_B. Trp_tRNA_synth_B.
InterPro	IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002306. Trp-tRNA-ligase. IPR024109. Trp-tRNA-ligase_bac-type. [Graphical view]
PANTHER	PTHR10055. PTHR10055. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01039. TRNASYNTHTRP.
TIGRFAMs	TIGR00233. trpS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYW_RHOBA

Accession

Primary (citable) accession number: Q7UQA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program