Reviewed, UniProtKB/Swiss-Prot Q7UPZ8 (PUR2_RHOBA)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	RB6616

Organism

Rhodopirellula baltica [Complete proteome] [HAMAP]

Taxonomic identifier

265606 [NCBI]

Taxonomic lineage

Bacteria › Planctomycetes › Planctomycetacia › Planctomycetales › Planctomycetaceae › Rhodopirellula

Protein attributes

Sequence length	437 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 437	437	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151475
Regions
	Domain	110 – 322	213	ATP-grasp
	Nucleotide binding	142 – 203	62	ATP By similarity
Sites
	Metal binding	292	1	Magnesium or manganese By similarity
	Metal binding	294	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7UPZ8-1 [UniParc]. Last modified February 7, 2006. Version 2. Checksum: FF709E7F10A19993	FASTA	437	46,501
	10 20 30 40 50 60 MSKYNVLIVG SGGREHALAW KVKQSQHVQN VFVAPGNAGT GMDATNVDLD PADHDAVIQF 70 80 90 100 110 120 AKENNVGLVI VGPEAPLVAG LVDALTDAGL RAFGPSKAAS ELEGSKVFCK NLLRSADIPT 130 140 150 160 170 180 ADYRTFRSAD DASRYIKDRF SEPTDPVNVV VKADGLAAGK GVVVCDTRSE ALEAIDRIAA 190 200 210 220 230 240 RKEFGAAGKE LIIEERLTGP EVSVLAITDG ETIVTLPPAQ DHKPANDGDT GPNTGGMGAY 250 260 270 280 290 300 CPAPVLDEET LAKVESSILV PVVHAMKRSR RPFKGVLYAG LMLTPAGPKV LEFNVRFGDP 310 320 330 340 350 360 ECQPLLMRLK TDLVEVMQAV VDGKLEETGP LEFDPRPAIC VVMASEGYPA DYEKGHAITG 370 380 390 400 410 420 IESADKMENV KVFHAGTQRV DGEVVNTGGR VLGVTAMGDS ISAAKLQAYK AVREIRWQGA 430 WCRKDISDKA LVTADKA

References

[1]

"Complete genome sequence of the marine planctomycete Pirellula sp. strain 1." Gloeckner F.O., Kube M., Bauer M., Teeling H., Lombardot T., Ludwig W., Gade D., Beck A., Borzym K., Heitmann K., Rabus R., Schlesner H., Amann R., Reinhardt R. Proc. Natl. Acad. Sci. U.S.A. 100:8298-8303(2003) [PubMed: 12835416] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX294144 Genomic DNA. Translation: CAD74908.1. Different initiation.
3D structure databases
HSSP	HSSP built from PDB template 1GSO based on UniProtKB P15640.
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus RB6616 in contig BX119912_GR.
KEGG	rba:RB6616.
NMPDR	fig|243090.1.peg.3702.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7UPZ8.
Enzyme and pathway databases
BioCyc	PSP117:RB6616-MON.
BRENDA	6.3.4.13. 280490.
Family and domain databases
HAMAP	MF_00138. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_RHOBA

Accession

Primary (citable) accession number: Q7UPZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	February 7, 2006
Last modified:	September 22, 2009
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)