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Q7UNZ2

- SYI_RHOBA

UniProt

Q7UNZ2 - SYI_RHOBA

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Rhodopirellula baltica (strain SH1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei745 – 7451ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:RB7259
OrganismiRhodopirellula baltica (strain SH1)
Taxonomic identifieri243090 [NCBI]
Taxonomic lineageiBacteriaPlanctomycetesPlanctomycetiaPlanctomycetalesPlanctomycetaceaeRhodopirellula
ProteomesiUP000001025: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12131213Isoleucine--tRNA ligasePRO_0000098557Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243090.RB7259.

Structurei

3D structure databases

ProteinModelPortaliQ7UNZ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi100 – 11011"HIGH" regionAdd
BLAST
Motifi742 – 7465"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
InParanoidiQ7UNZ2.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7UNZ2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPRQAPTRPR SPKKPRESDG GGGNVLQRIK LCVVLTFPSH SSGYHVSANS
60 70 80 90 100
TAAFRAPAAS PHFPTLEEQV LAFWDQHSIY EQSLARRANA PTFVFYEGPP
110 120 130 140 150
TANGMPHPGH CLTRAIKDVF PRYKTMRGYR CERKAGWDTH GLPVEVEVGK
160 170 180 190 200
ELGIHSKEEI EAYGVEPFIQ KCQSSVWRYM QEWQTLTRRL GFWVNLEEAY
210 220 230 240 250
VTYHQSYVES VWWSLKNLFD RGLLYQGHKI VWWWAQGGTA LSAGEVGQGY
260 270 280 290 300
REVADPSVYV LFPLVDQPER SLVVWTTTPW TLPSNMYAAV KPELEYAVVK
310 320 330 340 350
DSETGQELVL AAALVDSLAG KLKRELTVVD TISGESLVGQ RYQPPFEDYQ
360 370 380 390 400
TRLNDPTGEL VTGGKESLYW RVVAADFVTT DSGSGLVHLA PAFGEIDHEV
410 420 430 440 450
LVTERTRFVD GQRPELLCAV GPDGKFTDEF ASMKGVWVKE ADKTLTRTLR
460 470 480 490 500
ESGRLLHLEQ YLHDYPFCWR ADDDPLIQYP RESWFIRTTK FRDLMLKNNS
510 520 530 540 550
KIGWQPEHIR DGRFGNFLES NVDWALSRER YWGTPLPIWV CQSTGRMEAI
560 570 580 590 600
ESYEELLAKP GVDGTQVWDN AKAANPELPD DLRVHKPYID SVTYDSPFES
610 620 630 640 650
GARMQRVSEV IDCWYDSGAM PFAQWGWPHQ NDAQFQEQFP ADFISEAIDQ
660 670 680 690 700
TRGWFYSQLA ISTMLFGEGA SIREAGTKAN ELAPSRDSES DYPHPFRNCI
710 720 730 740 750
VLGLMLSQWY ESAGKEGQPK TVLLSESEAE QADGKFTKKT GKMSKSLRNY
760 770 780 790 800
RSPSEIFDKY GADAMRWYFF ANQAPWNSII YADQAIRDSI PEFLLRLWNT
810 820 830 840 850
YSFFSIYAEI DGFDPTSATD VDEQLSPESL ASAPTYRPIS ERSEIDRWIH
860 870 880 890 900
SELHRTLATV IDRMDAFDNY NACQAITNLL DGLSNWYVRR SRDRFWAPDS
910 920 930 940 950
DSPDKHDAYW TLYEVMLELT KVIAPFVPFL ADTLWQELTA PFGDKALKSV
960 970 980 990 1000
HLCDFPKPDA SRVDQKLSDS MRLLREIASM GRSARADAKL KVRLPLAKVE
1010 1020 1030 1040 1050
VILTDDSAID WLQSHDALVR EELNVKAVDY TTDGGDYVQY TIVPNFKRLG
1060 1070 1080 1090 1100
PKVGKNIPVV KKMLGEADGN QLLTQLQTAG FVELELASGP LKLDGEDIEV
1110 1120 1130 1140 1150
RLQAREGWAA AQGSGCVVVL NTEVTPELRR EGLAKDLIRG IQSQRKELDC
1160 1170 1180 1190 1200
QYTDRIRVAV ETSDAELQSA IEEHRTMICG ETLADDLVIG TLPNAQQVAI
1210
EGGELYVAKV SDK
Length:1,213
Mass (Da):137,200
Last modified:October 1, 2003 - v1
Checksum:i353E3470B13258B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX294145 Genomic DNA. Translation: CAD75273.1.
RefSeqiNP_867726.1. NC_005027.1.

Genome annotation databases

EnsemblBacteriaiCAD75273; CAD75273; RB7259.
GeneIDi1791528.
KEGGirba:RB7259.
PATRICi23248485. VBIRhoBal59814_3516.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX294145 Genomic DNA. Translation: CAD75273.1 .
RefSeqi NP_867726.1. NC_005027.1.

3D structure databases

ProteinModelPortali Q7UNZ2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243090.RB7259.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD75273 ; CAD75273 ; RB7259 .
GeneIDi 1791528.
KEGGi rba:RB7259.
PATRICi 23248485. VBIRhoBal59814_3516.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
InParanoidi Q7UNZ2.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SH1.

Entry informationi

Entry nameiSYI_RHOBA
AccessioniPrimary (citable) accession number: Q7UNZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2003
Last modified: November 26, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3