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Q7UNZ2

- SYI_RHOBA

UniProt

Q7UNZ2 - SYI_RHOBA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Rhodopirellula baltica (strain SH1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei745 – 7451ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:RB7259
    OrganismiRhodopirellula baltica (strain SH1)
    Taxonomic identifieri243090 [NCBI]
    Taxonomic lineageiBacteriaPlanctomycetesPlanctomycetiaPlanctomycetalesPlanctomycetaceaeRhodopirellula
    ProteomesiUP000001025: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12131213Isoleucine--tRNA ligasePRO_0000098557Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi243090.RB7259.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7UNZ2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi100 – 11011"HIGH" regionAdd
    BLAST
    Motifi742 – 7465"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiRVEHMVE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q7UNZ2-1 [UniParc]FASTAAdd to Basket

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    MPRQAPTRPR SPKKPRESDG GGGNVLQRIK LCVVLTFPSH SSGYHVSANS     50
    TAAFRAPAAS PHFPTLEEQV LAFWDQHSIY EQSLARRANA PTFVFYEGPP 100
    TANGMPHPGH CLTRAIKDVF PRYKTMRGYR CERKAGWDTH GLPVEVEVGK 150
    ELGIHSKEEI EAYGVEPFIQ KCQSSVWRYM QEWQTLTRRL GFWVNLEEAY 200
    VTYHQSYVES VWWSLKNLFD RGLLYQGHKI VWWWAQGGTA LSAGEVGQGY 250
    REVADPSVYV LFPLVDQPER SLVVWTTTPW TLPSNMYAAV KPELEYAVVK 300
    DSETGQELVL AAALVDSLAG KLKRELTVVD TISGESLVGQ RYQPPFEDYQ 350
    TRLNDPTGEL VTGGKESLYW RVVAADFVTT DSGSGLVHLA PAFGEIDHEV 400
    LVTERTRFVD GQRPELLCAV GPDGKFTDEF ASMKGVWVKE ADKTLTRTLR 450
    ESGRLLHLEQ YLHDYPFCWR ADDDPLIQYP RESWFIRTTK FRDLMLKNNS 500
    KIGWQPEHIR DGRFGNFLES NVDWALSRER YWGTPLPIWV CQSTGRMEAI 550
    ESYEELLAKP GVDGTQVWDN AKAANPELPD DLRVHKPYID SVTYDSPFES 600
    GARMQRVSEV IDCWYDSGAM PFAQWGWPHQ NDAQFQEQFP ADFISEAIDQ 650
    TRGWFYSQLA ISTMLFGEGA SIREAGTKAN ELAPSRDSES DYPHPFRNCI 700
    VLGLMLSQWY ESAGKEGQPK TVLLSESEAE QADGKFTKKT GKMSKSLRNY 750
    RSPSEIFDKY GADAMRWYFF ANQAPWNSII YADQAIRDSI PEFLLRLWNT 800
    YSFFSIYAEI DGFDPTSATD VDEQLSPESL ASAPTYRPIS ERSEIDRWIH 850
    SELHRTLATV IDRMDAFDNY NACQAITNLL DGLSNWYVRR SRDRFWAPDS 900
    DSPDKHDAYW TLYEVMLELT KVIAPFVPFL ADTLWQELTA PFGDKALKSV 950
    HLCDFPKPDA SRVDQKLSDS MRLLREIASM GRSARADAKL KVRLPLAKVE 1000
    VILTDDSAID WLQSHDALVR EELNVKAVDY TTDGGDYVQY TIVPNFKRLG 1050
    PKVGKNIPVV KKMLGEADGN QLLTQLQTAG FVELELASGP LKLDGEDIEV 1100
    RLQAREGWAA AQGSGCVVVL NTEVTPELRR EGLAKDLIRG IQSQRKELDC 1150
    QYTDRIRVAV ETSDAELQSA IEEHRTMICG ETLADDLVIG TLPNAQQVAI 1200
    EGGELYVAKV SDK 1213
    Length:1,213
    Mass (Da):137,200
    Last modified:October 1, 2003 - v1
    Checksum:i353E3470B13258B3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX294145 Genomic DNA. Translation: CAD75273.1.
    RefSeqiNP_867726.1. NC_005027.1.

    Genome annotation databases

    EnsemblBacteriaiCAD75273; CAD75273; RB7259.
    GeneIDi1791528.
    KEGGirba:RB7259.
    PATRICi23248485. VBIRhoBal59814_3516.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX294145 Genomic DNA. Translation: CAD75273.1 .
    RefSeqi NP_867726.1. NC_005027.1.

    3D structure databases

    ProteinModelPortali Q7UNZ2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243090.RB7259.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD75273 ; CAD75273 ; RB7259 .
    GeneIDi 1791528.
    KEGGi rba:RB7259.
    PATRICi 23248485. VBIRhoBal59814_3516.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi RVEHMVE.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SH1.

    Entry informationi

    Entry nameiSYI_RHOBA
    AccessioniPrimary (citable) accession number: Q7UNZ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3