Q7UIY1 (HMP_RHOBA) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 58.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Flavohemoprotein Alternative name(s): Flavohemoglobin Hemoglobin-like protein Nitric oxide dioxygenase Short name=NO oxygenase Short name=NOD EC=1.14.12.17 | ||||||
| Gene names |
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| Organism | Rhodopirellula baltica (strain SH1) | ||||||
| Taxonomic identifier | 243090 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Planctomycetes › Planctomycetacia › Planctomycetales › Planctomycetaceae › Rhodopirellula |
Protein attributes
| Sequence length | 408 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Catalytic activity | 2 NO + 2 O2 + NAD(P)H = 2 NO3- + NAD(P)+. HAMAP MF_01252 |
| Cofactor | Binds 1 heme B (iron-protoporphyrin IX) group per subunit By similarity. Binds 1 FAD per subunit By similarity. HAMAP MF_01252 |
| Domain | Consists of two distinct domains; an N-terminal heme-containing oxygen-binding domain and a C-terminal reductase domain with binding sites for FAD and NAD(P)H. HAMAP MF_01252 |
| Sequence similarities | Belongs to the globin family. Two-domain flavohemoproteins subfamily. In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain. |
| Sequence caution | The sequence CAD77481.1 differs from that shown. Reason: Erroneous initiation. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Oxygen transport Transport |
| Ligand | FAD Flavoprotein Heme Iron Metal-binding NAD NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | response to nitrosative stress Inferred from electronic annotation. Source: InterPro |
| Molecular function | FAD binding Inferred from electronic annotation. Source: InterPro heme bindingInferred from electronic annotation. Source: InterPro nitric oxide dioxygenase activityInferred from electronic annotation. Source: EC oxygen bindingInferred from electronic annotation. Source: InterPro oxygen transporter activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 408 | 408 | Flavohemoprotein HAMAP MF_01252 | PRO_0000052443 | |||||
Regions | |||||||||
| Domain | 152 – 263 | 112 | FAD-binding FR-type | ||||||
| Nucleotide binding | 205 – 208 | 4 | FAD By similarity | ||||||
| Nucleotide binding | 277 – 282 | 6 | NADP By similarity | ||||||
| Nucleotide binding | 398 – 401 | 4 | FAD By similarity | ||||||
| Region | 1 – 138 | 138 | Globin HAMAP MF_01252 | ||||||
| Region | 149 – 408 | 260 | Reductase HAMAP MF_01252 | ||||||
| Region | 267 – 408 | 142 | NAD or NADP-binding HAMAP MF_01252 | ||||||
Sites | |||||||||
| Active site | 95 | 1 | Charge relay system By similarity | ||||||
| Active site | 137 | 1 | Charge relay system By similarity | ||||||
| Metal binding | 85 | 1 | Iron (heme proximal ligand) By similarity | ||||||
| Binding site | 190 | 1 | FAD By similarity | ||||||
| Site | 29 | 1 | Involved in heme-bound ligand stabilization and O-O bond activation By similarity | ||||||
| Site | 84 | 1 | Influences the redox potential of the prosthetic heme and FAD groups By similarity | ||||||
| Site | 397 | 1 | Influences the redox potential of the prosthetic heme and FAD groups By similarity | ||||||
Sequences
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References
| [1] | "Complete genome sequence of the marine planctomycete Pirellula sp. strain 1." Gloeckner F.O., Kube M., Bauer M., Teeling H., Lombardot T., Ludwig W., Gade D., Beck A., Borzym K., Heitmann K., Rabus R., Schlesner H., Amann R., Reinhardt R. Proc. Natl. Acad. Sci. U.S.A. 100:8298-8303(2003) [PubMed: 12835416] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SH1. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BX294154 Genomic DNA. Translation: CAD77481.1. Different initiation. |
| RefSeq | NP_870404.1. NC_005027.1. |
3D structure databases | |
| ProteinModelPortal | Q7UIY1. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 1794152. |
| GenomeReviews | Gene locus RB12262 in contig BX119912_GR. |
| KEGG | rba:RB12262. |
| NMPDR | fig|243090.1.peg.6744. |
| PATRIC | 23253383. VBIRhoBal59814_5920. |
Phylogenomic databases | |
| HOGENOM | HBG623097. |
| PhylomeDB | Q7UIY1. |
| ProtClustDB | PRK13289. |
Enzyme and pathway databases | |
| BioCyc | PSP117:RB12262-MONOMER. |
Family and domain databases | |
| HAMAP | MF_01252. Hmp. [Tree] |
| InterPro | IPR017927. Fd_Rdtase_FAD-bd. IPR009050. Globin-like. IPR012292. Globin_dom. IPR000971. Globin_subset. IPR023950. Hmp. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view] |
| Gene3D | G3DSA:1.10.490.10. Globin_related. 1 hit. |
| KO | K05916. |
| Pfam | PF00970. FAD_binding_6. 1 hit. PF00042. Globin. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view] |
| PRINTS | PR00406. CYTB5RDTASE. |
| SUPFAM | SSF46458. Globin_like. 1 hit. SSF63380. Riboflavin_synthase_like_b-brl. 1 hit. |
| PROSITE | PS51384. FAD_FR. 1 hit. PS01033. GLOBIN. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | HMP_RHOBA | ||||||||
| Accession | Primary (citable) accession number: Q7UIY1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

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