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Protein

Vitamin B12-dependent ribonucleotide reductase

Gene

nrdJ

Organism
Rhodopirellula baltica (strain DSM 10527 / NCIMB 13988 / SH1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

adenosylcob(III)alaminBy similarityNote: 5'-deoxyadenosylcobalamine (coenzyme B12).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei169SubstrateBy similarity1
Binding sitei242Substrate; via amide nitrogenBy similarity1
Active sitei420Proton acceptorBy similarity1
Active sitei422Cysteine radical intermediateBy similarity1
Active sitei424Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processDNA synthesis
LigandCobalamin, Cobalt, Nucleotide-binding

Enzyme and pathway databases

BioCyciRBAL243090:G1GTC-7110-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin B12-dependent ribonucleotide reductase (EC:1.17.4.1)
Alternative name(s):
Ribonucleoside-diphosphate reductase NrdJ
Gene namesi
Name:nrdJ
Ordered Locus Names:RB12762
OrganismiRhodopirellula baltica (strain DSM 10527 / NCIMB 13988 / SH1)
Taxonomic identifieri243090 [NCBI]
Taxonomic lineageiBacteriaPlanctomycetesPlanctomycetiaPlanctomycetalesPlanctomycetaceaeRhodopirellula
Proteomesi
  • UP000001025 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002316551 – 1040Vitamin B12-dependent ribonucleotide reductaseAdd BLAST1040

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi214 ↔ 433Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ7UI46

Interactioni

Protein-protein interaction databases

STRINGi243090.RB12762

Structurei

3D structure databases

ProteinModelPortaliQ7UI46
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni213 – 214Substrate bindingBy similarity2
Regioni420 – 424Substrate bindingBy similarity5
Regioni604 – 608Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105BZH Bacteria
COG0209 LUCA
HOGENOMiHOG000021771
InParanoidiQ7UI46
KOiK00525
OMAiPQQVSAC
OrthoDBiPOG091H0FGZ

Family and domain databases

CDDicd02888 RNR_II_dimer, 1 hit
InterProiView protein in InterPro
IPR013678 RNR_2_N
IPR000788 RNR_lg_C
IPR013344 RNR_NrdJ/NrdZ
PfamiView protein in Pfam
PF08471 Ribonuc_red_2_N, 1 hit
PF02867 Ribonuc_red_lgC, 1 hit
PRINTSiPR01183 RIBORDTASEM1
TIGRFAMsiTIGR02504 NrdJ_Z, 1 hit

Sequencei

Sequence statusi: Complete.

Q7UI46-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTVLPESNA TSRIPANDPA LEKVDSEHGV EYAREKFGTA LRGDRAGLKF
60 70 80 90 100
ESKFCPDDGR TPFATTAWDL RSAAIKDESG NALFEQTDCE VPASWSQLAT
110 120 130 140 150
NVVVSKYFYG DPKNTDERER SVRQLIHRVT RTISDWGLAD GYFDTPEDGE
160 170 180 190 200
RFYRDLTWLS LHQHGAFNSP VWFNVGLHAQ YDVEGDMCNW HWDRTENTTA
210 220 230 240 250
QPDNPYEYPQ GSACFIQSVD DNMEDIMRLA CSEAMLFKFG SGTGTDLSTI
260 270 280 290 300
RSQREKLSGG GTPSGPLSFM RVYDSIAGVV KSGGKTRRAA KMQSLKVWHP
310 320 330 340 350
DILEFIECKW AEEKKAHALI REGYDSNFNG EAYASVCFQN ANLSVRLTDD
360 370 380 390 400
YMEAVRKNEN FQTRWITDKP TTEPPSYVAK ELLNKMAECA WHCGDPGVQY
410 420 430 440 450
DTTINKWHTC PNSGAINASN PCSEYMFLDD TACNLASINL MKFVQQDGSF
460 470 480 490 500
DHERFRAACR TFFIAQEILV DHASYPTEPI ARNSHKFRPL GLGYSNLGSV
510 520 530 540 550
IMTAGLPYDS DAARGMCGSL TALLHGEANR TSAELASVVG TFDGYVENEA
560 570 580 590 600
PMLRVMQMHR DACDQINDDG PAELKEAARE LWDGVLEIGE KYGFRNAQAT
610 620 630 640 650
VLAPTGTISF MMDCDTTGIE PDIALVKYKQ LAGGGMLKIV NQTVKLGLKT
660 670 680 690 700
LGYDADTIDE ILKYVDANDT IEGAPGLQDE HLAVFDCAFK PANGVRSIGW
710 720 730 740 750
RAHITMMAAA QPFLSGAISK TVNMPTDVTP QDIADAYFWG WELGLKAIAI
760 770 780 790 800
YRDGSKQSQP LNTKSDEQKA TDAAEAAKVE TVEKIVYKPR RERLPDTRQS
810 820 830 840 850
LTHKFSIAGH EGYLCVGLYP DGRPGEMFIT MAKEGSTIGG IMDSFGTALS
860 870 880 890 900
IAMQYGVPLE VIVNKFSHTR FEPMGHTSNK DIRIAKSVVD YIARWLGITF
910 920 930 940 950
MSGNDYSPSA EGAAKTGGNG PDLTTAPAGA TANNNSPVMA ELRADAGAAV
960 970 980 990 1000
ALVERATLLA SLQNGVSNGS ATNGHSNGQS AGGSSDGAVA ERAVDGLGGQ
1010 1020 1030 1040
ADQFSRFQTD APSCDNCGSI TVRNGNCYLC HNCGNSMGCS
Length:1,040
Mass (Da):113,464
Last modified:October 1, 2003 - v1
Checksum:iD640951F588F5C03
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX294155 Genomic DNA Translation: CAD77768.1
RefSeqiNP_870691.1, NC_005027.1
WP_011123898.1, NC_005027.1

Genome annotation databases

EnsemblBacteriaiCAD77768; CAD77768; RB12762
GeneIDi1793559
KEGGirba:RB12762
PATRICifig|243090.15.peg.6184

Similar proteinsi

Entry informationi

Entry nameiNRDJ_RHOBA
AccessioniPrimary (citable) accession number: Q7UI46
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: October 1, 2003
Last modified: May 23, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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