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Q7UI46 (NRDJ_RHOBA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vitamin B12-dependent ribonucleotide reductase

EC=1.17.4.1
Alternative name(s):
Ribonucleoside-diphosphate reductase NrdJ
Gene names
Name:nrdJ
Ordered Locus Names:RB12762
OrganismRhodopirellula baltica (strain SH1) [Reference proteome] [HAMAP]
Taxonomic identifier243090 [NCBI]
Taxonomic lineageBacteriaPlanctomycetesPlanctomycetiaPlanctomycetalesPlanctomycetaceaeRhodopirellula

Protein attributes

Sequence length1040 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen By similarity.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactor

5'-deoxyadenosylcobalamine (coenzyme B12) By similarity.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase class-2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10401040Vitamin B12-dependent ribonucleotide reductase
PRO_0000231655

Regions

Region213 – 2142Substrate binding By similarity
Region420 – 4245Substrate binding By similarity
Region604 – 6085Substrate binding By similarity

Sites

Active site4201Proton acceptor By similarity
Active site4221Cysteine radical intermediate By similarity
Active site4241Proton acceptor By similarity
Binding site1691Substrate By similarity
Binding site2421Substrate; via amide nitrogen By similarity

Amino acid modifications

Disulfide bond214 ↔ 433Redox-active By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7UI46 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: D640951F588F5C03

FASTA1,040113,464
        10         20         30         40         50         60 
MSTVLPESNA TSRIPANDPA LEKVDSEHGV EYAREKFGTA LRGDRAGLKF ESKFCPDDGR 

        70         80         90        100        110        120 
TPFATTAWDL RSAAIKDESG NALFEQTDCE VPASWSQLAT NVVVSKYFYG DPKNTDERER 

       130        140        150        160        170        180 
SVRQLIHRVT RTISDWGLAD GYFDTPEDGE RFYRDLTWLS LHQHGAFNSP VWFNVGLHAQ 

       190        200        210        220        230        240 
YDVEGDMCNW HWDRTENTTA QPDNPYEYPQ GSACFIQSVD DNMEDIMRLA CSEAMLFKFG 

       250        260        270        280        290        300 
SGTGTDLSTI RSQREKLSGG GTPSGPLSFM RVYDSIAGVV KSGGKTRRAA KMQSLKVWHP 

       310        320        330        340        350        360 
DILEFIECKW AEEKKAHALI REGYDSNFNG EAYASVCFQN ANLSVRLTDD YMEAVRKNEN 

       370        380        390        400        410        420 
FQTRWITDKP TTEPPSYVAK ELLNKMAECA WHCGDPGVQY DTTINKWHTC PNSGAINASN 

       430        440        450        460        470        480 
PCSEYMFLDD TACNLASINL MKFVQQDGSF DHERFRAACR TFFIAQEILV DHASYPTEPI 

       490        500        510        520        530        540 
ARNSHKFRPL GLGYSNLGSV IMTAGLPYDS DAARGMCGSL TALLHGEANR TSAELASVVG 

       550        560        570        580        590        600 
TFDGYVENEA PMLRVMQMHR DACDQINDDG PAELKEAARE LWDGVLEIGE KYGFRNAQAT 

       610        620        630        640        650        660 
VLAPTGTISF MMDCDTTGIE PDIALVKYKQ LAGGGMLKIV NQTVKLGLKT LGYDADTIDE 

       670        680        690        700        710        720 
ILKYVDANDT IEGAPGLQDE HLAVFDCAFK PANGVRSIGW RAHITMMAAA QPFLSGAISK 

       730        740        750        760        770        780 
TVNMPTDVTP QDIADAYFWG WELGLKAIAI YRDGSKQSQP LNTKSDEQKA TDAAEAAKVE 

       790        800        810        820        830        840 
TVEKIVYKPR RERLPDTRQS LTHKFSIAGH EGYLCVGLYP DGRPGEMFIT MAKEGSTIGG 

       850        860        870        880        890        900 
IMDSFGTALS IAMQYGVPLE VIVNKFSHTR FEPMGHTSNK DIRIAKSVVD YIARWLGITF 

       910        920        930        940        950        960 
MSGNDYSPSA EGAAKTGGNG PDLTTAPAGA TANNNSPVMA ELRADAGAAV ALVERATLLA 

       970        980        990       1000       1010       1020 
SLQNGVSNGS ATNGHSNGQS AGGSSDGAVA ERAVDGLGGQ ADQFSRFQTD APSCDNCGSI 

      1030       1040 
TVRNGNCYLC HNCGNSMGCS 

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References

[1]"Complete genome sequence of the marine planctomycete Pirellula sp. strain 1."
Gloeckner F.O., Kube M., Bauer M., Teeling H., Lombardot T., Ludwig W., Gade D., Beck A., Borzym K., Heitmann K., Rabus R., Schlesner H., Amann R., Reinhardt R.
Proc. Natl. Acad. Sci. U.S.A. 100:8298-8303(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SH1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX294155 Genomic DNA. Translation: CAD77768.1.
RefSeqNP_870691.1. NC_005027.1.

3D structure databases

ProteinModelPortalQ7UI46.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243090.RB12762.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD77768; CAD77768; RB12762.
GeneID1793559.
KEGGrba:RB12762.
PATRIC23253911. VBIRhoBal59814_6184.

Phylogenomic databases

eggNOGCOG0209.
HOGENOMHOG000021771.
KOK00525.
OMATAFDIKF.
OrthoDBEOG6H4K5F.
ProtClustDBPRK06556.

Family and domain databases

InterProIPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
IPR024434. TSCPD_dom.
[Graphical view]
PfamPF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF12637. TSCPD. 1 hit.
[Graphical view]
PRINTSPR01183. RIBORDTASEM1.
TIGRFAMsTIGR02504. NrdJ_Z. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNRDJ_RHOBA
AccessionPrimary (citable) accession number: Q7UI46
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: October 1, 2003
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families