Q7UI46 (NRDJ_RHOBA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vitamin B12-dependent ribonucleotide reductase
EC=1.17.4.1

Alternative name(s):
Ribonucleoside-diphosphate reductase NrdJ

Gene names

Name:	nrdJ
Ordered Locus Names:	RB12762

Organism

Rhodopirellula baltica (strain SH1) [Reference proteome] [HAMAP]

Taxonomic identifier

243090 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Planctomycetes › Planctomycetia › Planctomycetales › Planctomycetaceae › Rhodopirellula › Rhodopirellula baltica

Protein attributes

Sequence length	1040 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	5'-deoxyadenosylcobalamine (coenzyme B12) By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase class-2 family.

Ontologies

Keywords
Biological process	DNA synthesis
Ligand	Cobalamin Cobalt Nucleotide-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	cobalamin binding Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1040	1040	Vitamin B12-dependent ribonucleotide reductase		PRO_0000231655
Sites
	Active site	214	1	By similarity
	Active site	422	1	By similarity
	Active site	433	1	By similarity
	Active site	1033	1	By similarity
	Active site	1039	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7UI46 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: D640951F588F5C03	FASTA	1,040	113,464
	10 20 30 40 50 60 MSTVLPESNA TSRIPANDPA LEKVDSEHGV EYAREKFGTA LRGDRAGLKF ESKFCPDDGR 70 80 90 100 110 120 TPFATTAWDL RSAAIKDESG NALFEQTDCE VPASWSQLAT NVVVSKYFYG DPKNTDERER 130 140 150 160 170 180 SVRQLIHRVT RTISDWGLAD GYFDTPEDGE RFYRDLTWLS LHQHGAFNSP VWFNVGLHAQ 190 200 210 220 230 240 YDVEGDMCNW HWDRTENTTA QPDNPYEYPQ GSACFIQSVD DNMEDIMRLA CSEAMLFKFG 250 260 270 280 290 300 SGTGTDLSTI RSQREKLSGG GTPSGPLSFM RVYDSIAGVV KSGGKTRRAA KMQSLKVWHP 310 320 330 340 350 360 DILEFIECKW AEEKKAHALI REGYDSNFNG EAYASVCFQN ANLSVRLTDD YMEAVRKNEN 370 380 390 400 410 420 FQTRWITDKP TTEPPSYVAK ELLNKMAECA WHCGDPGVQY DTTINKWHTC PNSGAINASN 430 440 450 460 470 480 PCSEYMFLDD TACNLASINL MKFVQQDGSF DHERFRAACR TFFIAQEILV DHASYPTEPI 490 500 510 520 530 540 ARNSHKFRPL GLGYSNLGSV IMTAGLPYDS DAARGMCGSL TALLHGEANR TSAELASVVG 550 560 570 580 590 600 TFDGYVENEA PMLRVMQMHR DACDQINDDG PAELKEAARE LWDGVLEIGE KYGFRNAQAT 610 620 630 640 650 660 VLAPTGTISF MMDCDTTGIE PDIALVKYKQ LAGGGMLKIV NQTVKLGLKT LGYDADTIDE 670 680 690 700 710 720 ILKYVDANDT IEGAPGLQDE HLAVFDCAFK PANGVRSIGW RAHITMMAAA QPFLSGAISK 730 740 750 760 770 780 TVNMPTDVTP QDIADAYFWG WELGLKAIAI YRDGSKQSQP LNTKSDEQKA TDAAEAAKVE 790 800 810 820 830 840 TVEKIVYKPR RERLPDTRQS LTHKFSIAGH EGYLCVGLYP DGRPGEMFIT MAKEGSTIGG 850 860 870 880 890 900 IMDSFGTALS IAMQYGVPLE VIVNKFSHTR FEPMGHTSNK DIRIAKSVVD YIARWLGITF 910 920 930 940 950 960 MSGNDYSPSA EGAAKTGGNG PDLTTAPAGA TANNNSPVMA ELRADAGAAV ALVERATLLA 970 980 990 1000 1010 1020 SLQNGVSNGS ATNGHSNGQS AGGSSDGAVA ERAVDGLGGQ ADQFSRFQTD APSCDNCGSI 1030 1040 TVRNGNCYLC HNCGNSMGCS

References

[1]

"Complete genome sequence of the marine planctomycete Pirellula sp. strain 1." Gloeckner F.O., Kube M., Bauer M., Teeling H., Lombardot T., Ludwig W., Gade D., Beck A., Borzym K., Heitmann K., Rabus R., Schlesner H., Amann R., Reinhardt R. Proc. Natl. Acad. Sci. U.S.A. 100:8298-8303(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SH1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX294155 Genomic DNA. Translation: CAD77768.1.
RefSeq	NP_870691.1. NC_005027.1.
3D structure databases
ProteinModelPortal	Q7UI46.
ModBase	Search...
Protein-protein interaction databases
STRING	243090.RB12762.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD77768; CAD77768; RB12762.
GeneID	1793559.
KEGG	rba:RB12762.
PATRIC	23253911. VBIRhoBal59814_6184.
Phylogenomic databases
eggNOG	COG0209.
HOGENOM	HOG000021771.
KO	K00525.
OMA	FLWRSVP.
ProtClustDB	PRK06556.
Family and domain databases
InterPro	IPR013678. RNR_2_N. IPR000788. RNR_lg_C. IPR013344. RNR_NrdJ/NrdZ. IPR024434. TSCPD_dom. [Graphical view]
Pfam	PF08471. Ribonuc_red_2_N. 1 hit. PF02867. Ribonuc_red_lgC. 1 hit. PF12637. TSCPD. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
TIGRFAMs	TIGR02504. NrdJ_Z. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NRDJ_RHOBA

Accession

Primary (citable) accession number: Q7UI46

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program