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Q7UF84 (BIOB_RHOBA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:RB10275
OrganismRhodopirellula baltica (strain SH1) [Reference proteome] [HAMAP]
Taxonomic identifier243090 [NCBI]
Taxonomic lineageBacteriaPlanctomycetesPlanctomycetiaPlanctomycetalesPlanctomycetaceaeRhodopirellula

Protein attributes

Sequence length359 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 359359Biotin synthase HAMAP-Rule MF_01694
PRO_0000381578

Sites

Metal binding941Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding981Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1011Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1381Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1701Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2301Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding3001Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q7UF84 [UniParc].

Last modified October 1, 2003. Version 1.
Checksum: AE6E1BDF18BE5482

FASTA35938,977
        10         20         30         40         50         60 
MSVADSSAAD SVAAPDTADT SSSVGVFSPP GYYDALAQQV LDGTPITREQ ALAMLEASDL 

        70         80         90        100        110        120 
DVPAIISAGY RIRHQYFGNT VQLYFLMNAK SGLCPEDCHY CSQSKVSDAP VPKYNILKRD 

       130        140        150        160        170        180 
ALMDAAKVAA ERGAKTYCLV ISARGPNERE MKAVEEIVPE IKQKYNLDIC ACLGLLDESQ 

       190        200        210        220        230        240 
AARLKACGVD RVNHNLNSSE SHYEKICTTH TYEDRVQTLR HVRDAGMEMC SGGIIGMGES 

       250        260        270        280        290        300 
KSDIVSMAFD LNELGVQSIP VNILNAIDGT PLEGTAALTP QDALKALAMF RFVNPDRELR 

       310        320        330        340        350 
IAGGRELHLR QLQPMGLYVA NSVFVGDYLT TQGQAPQADY DMIRDLGFDV TGSCEEMSI 

« Hide

References

[1]"Complete genome sequence of the marine planctomycete Pirellula sp. strain 1."
Gloeckner F.O., Kube M., Bauer M., Teeling H., Lombardot T., Ludwig W., Gade D., Beck A., Borzym K., Heitmann K., Rabus R., Schlesner H., Amann R., Reinhardt R.
Proc. Natl. Acad. Sci. U.S.A. 100:8298-8303(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SH1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX294151 Genomic DNA. Translation: CAD78799.1.
RefSeqNP_869342.1. NC_005027.1.

3D structure databases

ProteinModelPortalQ7UF84.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243090.RB10275.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD78799; CAD78799; RB10275.
GeneID1794717.
KEGGrba:RB10275.
PATRIC23251417. VBIRhoBal59814_4968.

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMANCRFCAQ.
OrthoDBEOG622PMP.
ProtClustDBPRK06256.

Enzyme and pathway databases

UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_RHOBA
AccessionPrimary (citable) accession number: Q7UF84
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: October 1, 2003
Last modified: February 19, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways