Q7UEV3 (ISPE_RHOBA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	RB10537

Organism

Rhodopirellula baltica (strain SH1)

Taxonomic identifier

243090 [NCBI]

Taxonomic lineage

Bacteria › Planctomycetes › Planctomycetacia › Planctomycetales › Planctomycetaceae › Rhodopirellula

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000189255
Regions
	Nucleotide binding	117 – 127	11	ATP Potential
Sites
	Active site	14	1	By similarity
	Active site	166	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7UEV3 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 1A1FA309DB2214CD	FASTA	329	35,299
	10 20 30 40 50 60 MTESRWYSTS PPAKLNLFLE LLARRDDGFH ELDTVMVAID WRDELHVRRT TNPGVQLSVD 70 80 90 100 110 120 WTDGRTSVAA ELQVDEDNEL LLVPTDDKNL VVRALNRLTE TLRLDGGWEV QLNKNIPSGA 130 140 150 160 170 180 GMGGASSDAA SALQLGWAAA RETQSDLPET LKNETLLSLA AEIGSDVPFF LGDLQADRLV 190 200 210 220 230 240 QCAHATGRGE KLNFFTLANP LHAVVIYPAA SVSTAQVYSR CQVPDSPQSS NELLRALQGI 250 260 270 280 290 300 AAESEFSLHN ALQAPARGLS SRIDPALECL SKAGLTHCHM TGSGSACFAL ADSSQQAAIA 310 320 AETLRSTFPG GALIRPVMSC VVPSEIHIA

References

[1]

"Complete genome sequence of the marine planctomycete Pirellula sp. strain 1." Gloeckner F.O., Kube M., Bauer M., Teeling H., Lombardot T., Ludwig W., Gade D., Beck A., Borzym K., Heitmann K., Rabus R., Schlesner H., Amann R., Reinhardt R. Proc. Natl. Acad. Sci. U.S.A. 100:8298-8303(2003) [PubMed: 12835416] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SH1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX294151 Genomic DNA. Translation: CAD78931.1.
RefSeq	NP_869474.1. NC_005027.1.
3D structure databases
ProteinModelPortal	Q7UEV3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1794233.
GenomeReviews	Gene locus RB10537 in contig BX119912_GR.
KEGG	rba:RB10537.
NMPDR	fig|243090.1.peg.5814.
PATRIC	23251661. VBIRhoBal59814_5090.
Phylogenomic databases
OMA	GPFAKGL.
PhylomeDB	Q7UEV3.
Enzyme and pathway databases
BioCyc	PSP117:RB10537-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_RHOBA

Accession

Primary (citable) accession number: Q7UEV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program