Reviewed, UniProtKB/Swiss-Prot Q7UBI7 (GLNE_SHIFL)

Names and origin

Protein names

Recommended name:
    Glutamate-ammonia-ligase adenylyltransferase
    EC=2.7.7.42
Alternative name(s):
    [Glutamate--ammonia-ligase] adenylyltransferase
    Glutamine-synthetase adenylyltransferase
      Short name=ATase

Gene names

Name:	glnE
Ordered Locus Names:	SF3094, S3299

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	946 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Adenylation and deadenylation of glutamine synthetase By similarity.
Catalytic activity	ATP + [L-glutamate:ammonia ligase (ADP-forming)] = diphosphate + adenylyl-[L-glutamate:ammonia ligase (ADP-forming)]. HAMAP MF_00802
Sequence similarities	Belongs to the glnE family.

Ontologies

Keywords
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [glutamate-ammonia-ligase] adenylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 946	946	Glutamate-ammonia-ligase adenylyltransferase HAMAP MF_00802		PRO_0000209278
Regions
	Region	91 – 302	212	GlnE 1 HAMAP MF_00802
	Region	609 – 830	222	GlnE 2 HAMAP MF_00802

Sequences

	Sequence		Length	Mass (Da)
	Q7UBI7-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 0E3030A2FA5466D9	FASTA	946	108,391
	10 20 30 40 50 60 MKPLSSPLQQ YWQTVVERLP ELLAEESLSA QAKSVLTFSD FVQDSVIAHP EWLTELESQP 70 80 90 100 110 120 PQADEWQHYS VWLQEALSNV SDEAGLMREL RLFRRRIMVR IAWAQTLALV TEESILQQLS 130 140 150 160 170 180 YLAETLIVAA RDWLYDACCR EWGTPCNAQG EAQPLLILGM GKLGGGELNF SSDIDLIFAW 190 200 210 220 230 240 PEHGCTQGGR RELDNAQFFT RMGQRLIKVL DQPTQDGFVY RVDMRLRPFG ESGPLVLSFA 250 260 270 280 290 300 ALEDYYQEQG RDWERYAMVK ARIMGDSEGV YANELRAMLR PFVFRRYIDF SVIQSLRNMK 310 320 330 340 350 360 GMIAREVRRR GLTDNIKLGA GGIREIEFIV QVFQLIRGGR EPLLQSRSLL PTLSAIAALH 370 380 390 400 410 420 LLSENDAEQL RVAYLFLRRL ENLLQSINDE QTQTLPSDEL NRARLAWAMD FADWPQLTGA 430 440 450 460 470 480 LTAHMTNVRR VFNELIGDDE SETQEESLSE QWRELWQDAL QEDDTTPVLA HLSEDDRKQV 490 500 510 520 530 540 LTMIADFRKE LDKRTIGPRG RQVLDHLMPH LLSDVCARED AAVTLSRITA LLVGIVTRTT 550 560 570 580 590 600 YLELLSEFPA ALKHLISLCA ASPMIASQLA RYPLLLDELL DPNTLYQPTA TDAYRDELRQ 610 620 630 640 650 660 YLLRVPEDDE EQQLEALRQF KQAQLLRIAA ADIAGTLPVM KVSDHLTWLA EAMIDAVVQQ 670 680 690 700 710 720 AWGQMVARYG KPNHLNEREG RGFAVVGYGK LGGWELGYSS DLDLIFLHDC PMDAMTDGER 730 740 750 760 770 780 EIDGRQFYLR LAQRIMHLFS TRTSSGILYE VDARLRPSGA AGMLVTSAEA FADYQKNEAW 790 800 810 820 830 840 TWEHQALVRA RVVYGDPQLT AHFDAVRREI MTLPREGKTL QTEVREMREK MRAHLGNKHR 850 860 870 880 890 900 NRFDIKADEG GITDIEFITQ YLVLRYAHEK PKLTRWSDNV RILELLAQND IMEEQEAMAL 910 920 930 940 TRAYTTLRDE LHHLALQELP GHVSEDCFTA ERDLVRASWQ KWLVEE

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005674 Genomic DNA. Translation: AAN44570.1. Different initiation. AE014073 Genomic DNA. Translation: AAP18382.1.
RefSeq	NP_708863.2. NP_838572.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1026840. 1079543.
GenomeReviews	Gene locus SF3094 in contig AE005674_GR. Gene locus S3299 in contig AE014073_GR.
KEGG	sfl:SF3094. sfx:S3299.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7UBI7.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN44570.1-MON.
BRENDA	2.7.7.42. 189495.
Family and domain databases
HAMAP	MF_00802. [Tree]
InterPro	IPR005190. GlnE. IPR013546. PII_UdlTrfase/GS_AdlTrfase. [Graphical view]
Pfam	PF08335. GlnD_UR_UTase. 1 hit. PF03710. GlnE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLNE_SHIFL

Accession

Primary (citable) accession number: Q7UBI7
Secondary accession number(s): Q83Q46

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	October 1, 2003
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)