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Protein

Ribosomal RNA small subunit methyltransferase J

Gene

rsmJ

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the guanosine in position 1516 of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(1516) in 16S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(1516) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei171 – 1711S-adenosyl-L-methionine1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase JUniRule annotation (EC:2.1.1.242UniRule annotation)
Alternative name(s):
16S rRNA m2G1516 methyltransferaseUniRule annotation
rRNA (guanine-N(2)-)-methyltransferaseUniRule annotation
Gene namesi
Name:rsmJUniRule annotation
Synonyms:yhiQ
Ordered Locus Names:SF3528, S4240
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 250250Ribosomal RNA small subunit methyltransferase JPRO_0000212093Add
BLAST

Proteomic databases

PaxDbiQ7UAV7.

Interactioni

Protein-protein interaction databases

STRINGi198214.SF3528.

Structurei

Secondary structure

1
250
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 76Combined sources
Helixi15 – 239Combined sources
Beta strandi32 – 376Combined sources
Beta strandi42 – 465Combined sources
Helixi50 – 523Combined sources
Beta strandi59 – 613Combined sources
Helixi62 – 709Combined sources
Helixi73 – 753Combined sources
Helixi77 – 815Combined sources
Beta strandi92 – 943Combined sources
Helixi101 – 1099Combined sources
Beta strandi113 – 1175Combined sources
Helixi120 – 13516Combined sources
Turni137 – 1393Combined sources
Helixi140 – 1467Combined sources
Beta strandi147 – 1526Combined sources
Helixi154 – 1574Combined sources
Beta strandi166 – 1705Combined sources
Helixi185 – 1939Combined sources
Helixi200 – 2034Combined sources
Helixi204 – 2107Combined sources
Beta strandi212 – 2209Combined sources
Helixi226 – 2283Combined sources
Beta strandi232 – 2365Combined sources
Beta strandi238 – 2469Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OYRX-ray2.00A1-250[»]
ProteinModelPortaliQ7UAV7.
SMRiQ7UAV7. Positions 1-250.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7UAV7.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni101 – 1022S-adenosyl-L-methionine binding1 Publication
Regioni117 – 1182S-adenosyl-L-methionine binding1 Publication
Regioni153 – 1542S-adenosyl-L-methionine binding1 Publication

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RsmJ family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105FFN. Bacteria.
ENOG410XPRJ. LUCA.
HOGENOMiHOG000218236.
KOiK15984.
OMAiSSRYDIY.
OrthoDBiEOG657J92.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01523. 16SrRNA_methyltr_J.
InterProiIPR007536. 16SrRNA_methylTrfase_J.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF04445. SAM_MT. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7UAV7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKICLIDETG AGDGALSVLA ARWGLEHDED NLMALVLTPE HLELRKRDEP
60 70 80 90 100
KLGGIFVDFV GGAMAHRRKF GGGRGEAVAK AVGIKGDYLP DVVDATAGLG
110 120 130 140 150
RDAFVLASVG CRVRMLERNP VVAALLDDGL ARGYADAEIG GWLQERLQLI
160 170 180 190 200
HASSLTALTD ITPRPQVVYL DPMFPHKQKS ALVKKEMRVF QSLVGPDLDA
210 220 230 240 250
DGLLEPARLL ATKRVVVKRP DYAPPLANVA TPNAVVTKGH RFDIYAGTPV
Length:250
Mass (Da):26,919
Last modified:October 1, 2003 - v1
Checksum:i7826E98CBAC1C978
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN44984.2.
AE014073 Genomic DNA. Translation: AAP19202.1.
RefSeqiNP_709277.2. NC_004337.2.
WP_000686608.1. NZ_LM651928.1.

Genome annotation databases

EnsemblBacteriaiAAN44984; AAN44984; SF3528.
AAP19202; AAP19202; S4240.
GeneIDi1026390.
KEGGisfl:SF3528.
sfx:S4240.
PATRICi18710458. VBIShiFle31049_4633.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN44984.2.
AE014073 Genomic DNA. Translation: AAP19202.1.
RefSeqiNP_709277.2. NC_004337.2.
WP_000686608.1. NZ_LM651928.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OYRX-ray2.00A1-250[»]
ProteinModelPortaliQ7UAV7.
SMRiQ7UAV7. Positions 1-250.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198214.SF3528.

Proteomic databases

PaxDbiQ7UAV7.

Protocols and materials databases

DNASUi1080448.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN44984; AAN44984; SF3528.
AAP19202; AAP19202; S4240.
GeneIDi1026390.
KEGGisfl:SF3528.
sfx:S4240.
PATRICi18710458. VBIShiFle31049_4633.

Phylogenomic databases

eggNOGiENOG4105FFN. Bacteria.
ENOG410XPRJ. LUCA.
HOGENOMiHOG000218236.
KOiK15984.
OMAiSSRYDIY.
OrthoDBiEOG657J92.

Miscellaneous databases

EvolutionaryTraceiQ7UAV7.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01523. 16SrRNA_methyltr_J.
InterProiIPR007536. 16SrRNA_methylTrfase_J.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF04445. SAM_MT. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
    Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
    , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
    Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 301 / Serotype 2a.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700930 / 2457T / Serotype 2a.
  3. "Crystal structure of UPF0341 protein (YhiQ) from Shigella flexneri in complex with S-adenosyl homocysteine, northeast structural genomics target sfr275."
    Northeast structural genomics consortium (NESG)
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE.
    Strain: ATCC 700930 / 2457T / Serotype 2a.

Entry informationi

Entry nameiRSMJ_SHIFL
AccessioniPrimary (citable) accession number: Q7UAV7
Secondary accession number(s): Q83J64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: October 1, 2003
Last modified: January 20, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.