Q7U9K4 (ISPH_SYNPX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Synonyms:	lytB
Ordered Locus Names:	SYNW0252

Organism

Synechococcus sp. (strain WH8102) [Complete proteome] [HAMAP]

Taxonomic identifier

84588 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 399	399	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_0000128879

Sequences

	Sequence		Length	Mass (Da)
	Q7U9K4 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: F6EA4285600F0836	FASTA	399	44,609
	10 20 30 40 50 60 MDTHAFKRSL HHSERYNRRG FGRAEEVAES LEQAYQSGLI GTIRDNGYKL SHGRLTVRLA 70 80 90 100 110 120 EAFGFCWGVE RAVAMAYETR KHYPAERLWI TNEIIHNPSV NDHLREMDVQ FIPVEQGVKD 130 140 150 160 170 180 FSGVTSGDVV ILPAFGATVQ EMQLLNERGC HIVDTTCPWV SKVWTTVEKH KKHTITSIIH 190 200 210 220 230 240 GKVKHEETLA TSSFAGTYLV VLDMEEAQIV ADYILGKGDR DAFMQRFSAA CSPGFDPDRD 250 260 270 280 290 300 LSRLGVANQT TMLKSETEEI GRLFERTMLS KYGPAELNDH FVAFNTICDA TQERQDAMFS 310 320 330 340 350 360 LVDEPLDLMV VIGGFNSSNT THLQEIALSR GIRSFHIDTP DRLDAQANAI EHKPLNENLR 370 380 390 LESNFLPAGP VTVGITSGAS TPDRAVEEVI EKLMLLSES

References

[1]

"The genome of a motile marine Synechococcus." Palenik B., Brahamsha B., Larimer F.W., Land M.L., Hauser L., Chain P., Lamerdin J.E., Regala W., Allen E.E., McCarren J., Paulsen I.T., Dufresne A., Partensky F., Webb E.A., Waterbury J. Nature 424:1037-1042(2003) [PubMed: 12917641] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WH8102.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX569689 Genomic DNA. Translation: CAE06767.1.
RefSeq	NP_896347.1. NC_005070.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q7U9K4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1730516.
GenomeReviews	Gene locus SYNW0252 in contig BX548020_GR.
KEGG	syw:SYNW0252.
PATRIC	23831883. VBISynSp27240_0255.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0761.
HOGENOM	HBG286221.
OMA	VQEMQLL.
PhylomeDB	Q7U9K4.
ProtClustDB	PRK13371.
Enzyme and pathway databases
BioCyc	SSP84588:SYNW0252OR2265-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_SYNPX

Accession

Primary (citable) accession number: Q7U9K4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program