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Q7U9G9

- SYI_SYNPX

UniProt

Q7U9G9 - SYI_SYNPX

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Synechococcus sp. (strain WH8102)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei585 – 5851Aminoacyl-adenylateUniRule annotation
Binding sitei629 – 6291ATPUniRule annotation
Metal bindingi939 – 9391ZincUniRule annotation
Metal bindingi942 – 9421ZincUniRule annotation
Metal bindingi959 – 9591ZincUniRule annotation
Metal bindingi962 – 9621ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:SYNW0289
OrganismiSynechococcus sp. (strain WH8102)
Taxonomic identifieri84588 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
ProteomesiUP000001422: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 974974Isoleucine--tRNA ligasePRO_0000098491Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi84588.SYNW0289.

Structurei

3D structure databases

ProteinModelPortaliQ7U9G9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi69 – 7911"HIGH" regionAdd
BLAST
Motifi626 – 6305"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7U9G9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKETRDAAA EGRPSYKDTL NLLQTGFGMR ANAVKREPEL QAFWSDNGID
60 70 80 90 100
GQLGLQNDGP TFTLHDGPPY ANGALHMGHA LNKVLKDVIN KYQVLKGRRV
110 120 130 140 150
RYVPGWDCHG LPIELKVLQS MDQEQRKALT PIKLRKKAAA YARKQVDGQM
160 170 180 190 200
KGFQRWGIWA DWEQPYLTLQ KEYEAAQIKV FGEMVLKGHI YRGLKPVHWS
210 220 230 240 250
PSSRTALAEA ELEYPDGHTS PSVYVAFPAM EVPTPLRDAL KAEGLELPTE
260 270 280 290 300
TDALRQALQV AIWTTTPWTL PANLAVSVNE RLDYALVDDG SGRMLVVAAD
310 320 330 340 350
LIESLSTTLE RPLKHRATVK GALLAGLIYR HPLLDRTSPV VIGGEYITTE
360 370 380 390 400
SGTGLVHTAP GHGVDDFHTG QKHGLPVLCP VDEAGTLTAE AGPFAGLNVL
410 420 430 440 450
KDANPGIIEA LEQAGALLKQ EAYSHRYPYD WRTKKPTIFR ATEQWFASVE
460 470 480 490 500
GFRQDALDAI DQVQWTPASG RNRIEAMVKE RGDWCISRQR TWGVPIPVFY
510 520 530 540 550
HHSNGEVLLN ADTLSHIETL IASHGADVWW EKDETDLLPP AYANQADQWR
560 570 580 590 600
KGTDTMDVWF DSGSSWAAVS SQRESLSYPA DLYLEGSDQH RGWFQSSLLT
610 620 630 640 650
SVAVNGHAPY KRVLTHGFAL DEKGRKMSKS LGNVVDPMVI IEGGKNQKQE
660 670 680 690 700
PPYGADVLRL WVSSVDYSAD VPIGAGILRQ LADVYRKVRN TSRYLLGNLH
710 720 730 740 750
DFNPASDAIA VADLPLLDRW MLQRTAEVMH EITEAFESYE FFRFFQLLQN
760 770 780 790 800
FCVTDLSNFY LDIAKDRLYV SAPTDQRRRS CQTVMALIIE RLAGFIAPVL
810 820 830 840 850
CHMAEDIWQN LPYPVQETSV FQRGWPTIPS DWRNDTLSAP VQQLRDLRAA
860 870 880 890 900
VNKVLEDCRG RQELGASLEA AVRIDARSPE LQAALSWLND NGDPDVDGLR
910 920 930 940 950
DWLLVSQLQL GGEPWAEVLS NHEDELALIE VSRARGTKCE RCWHYEGDVG
960 970
QHPDHAHICG RCVGVLERRT HQLV
Length:974
Mass (Da):108,998
Last modified:October 1, 2003 - v1
Checksum:iDF0058516CAE6565
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX569689 Genomic DNA. Translation: CAE06804.1.
RefSeqiNP_896384.1. NC_005070.1.
WP_011127163.1. NC_005070.1.

Genome annotation databases

EnsemblBacteriaiCAE06804; CAE06804; SYNW0289.
GeneIDi1732105.
KEGGisyw:SYNW0289.
PATRICi23831959. VBISynSp27240_0292.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX569689 Genomic DNA. Translation: CAE06804.1 .
RefSeqi NP_896384.1. NC_005070.1.
WP_011127163.1. NC_005070.1.

3D structure databases

ProteinModelPortali Q7U9G9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 84588.SYNW0289.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE06804 ; CAE06804 ; SYNW0289 .
GeneIDi 1732105.
KEGGi syw:SYNW0289.
PATRICi 23831959. VBISynSp27240_0292.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WH8102.

Entry informationi

Entry nameiSYI_SYNPX
AccessioniPrimary (citable) accession number: Q7U9G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2003
Last modified: November 26, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3