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Q7U9G9

- SYI_SYNPX

UniProt

Q7U9G9 - SYI_SYNPX

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Protein

Isoleucine--tRNA ligase

Gene
ileS, SYNW0289
Organism
Synechococcus sp. (strain WH8102)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei585 – 5851Aminoacyl-adenylate By similarity
Binding sitei629 – 6291ATP By similarity
Metal bindingi939 – 9391Zinc By similarity
Metal bindingi942 – 9421Zinc By similarity
Metal bindingi959 – 9591Zinc By similarity
Metal bindingi962 – 9621Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:SYNW0289
OrganismiSynechococcus sp. (strain WH8102)
Taxonomic identifieri84588 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
ProteomesiUP000001422: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 974974Isoleucine--tRNA ligaseUniRule annotationPRO_0000098491Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi84588.SYNW0289.

Structurei

3D structure databases

ProteinModelPortaliQ7U9G9.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi69 – 7911"HIGH" regionUniRule annotationAdd
BLAST
Motifi626 – 6305"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7U9G9-1 [UniParc]FASTAAdd to Basket

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MSKETRDAAA EGRPSYKDTL NLLQTGFGMR ANAVKREPEL QAFWSDNGID    50
GQLGLQNDGP TFTLHDGPPY ANGALHMGHA LNKVLKDVIN KYQVLKGRRV 100
RYVPGWDCHG LPIELKVLQS MDQEQRKALT PIKLRKKAAA YARKQVDGQM 150
KGFQRWGIWA DWEQPYLTLQ KEYEAAQIKV FGEMVLKGHI YRGLKPVHWS 200
PSSRTALAEA ELEYPDGHTS PSVYVAFPAM EVPTPLRDAL KAEGLELPTE 250
TDALRQALQV AIWTTTPWTL PANLAVSVNE RLDYALVDDG SGRMLVVAAD 300
LIESLSTTLE RPLKHRATVK GALLAGLIYR HPLLDRTSPV VIGGEYITTE 350
SGTGLVHTAP GHGVDDFHTG QKHGLPVLCP VDEAGTLTAE AGPFAGLNVL 400
KDANPGIIEA LEQAGALLKQ EAYSHRYPYD WRTKKPTIFR ATEQWFASVE 450
GFRQDALDAI DQVQWTPASG RNRIEAMVKE RGDWCISRQR TWGVPIPVFY 500
HHSNGEVLLN ADTLSHIETL IASHGADVWW EKDETDLLPP AYANQADQWR 550
KGTDTMDVWF DSGSSWAAVS SQRESLSYPA DLYLEGSDQH RGWFQSSLLT 600
SVAVNGHAPY KRVLTHGFAL DEKGRKMSKS LGNVVDPMVI IEGGKNQKQE 650
PPYGADVLRL WVSSVDYSAD VPIGAGILRQ LADVYRKVRN TSRYLLGNLH 700
DFNPASDAIA VADLPLLDRW MLQRTAEVMH EITEAFESYE FFRFFQLLQN 750
FCVTDLSNFY LDIAKDRLYV SAPTDQRRRS CQTVMALIIE RLAGFIAPVL 800
CHMAEDIWQN LPYPVQETSV FQRGWPTIPS DWRNDTLSAP VQQLRDLRAA 850
VNKVLEDCRG RQELGASLEA AVRIDARSPE LQAALSWLND NGDPDVDGLR 900
DWLLVSQLQL GGEPWAEVLS NHEDELALIE VSRARGTKCE RCWHYEGDVG 950
QHPDHAHICG RCVGVLERRT HQLV 974
Length:974
Mass (Da):108,998
Last modified:October 1, 2003 - v1
Checksum:iDF0058516CAE6565
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX569689 Genomic DNA. Translation: CAE06804.1.
RefSeqiNP_896384.1. NC_005070.1.
WP_011127163.1. NC_005070.1.

Genome annotation databases

EnsemblBacteriaiCAE06804; CAE06804; SYNW0289.
GeneIDi1732105.
KEGGisyw:SYNW0289.
PATRICi23831959. VBISynSp27240_0292.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX569689 Genomic DNA. Translation: CAE06804.1 .
RefSeqi NP_896384.1. NC_005070.1.
WP_011127163.1. NC_005070.1.

3D structure databases

ProteinModelPortali Q7U9G9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 84588.SYNW0289.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAE06804 ; CAE06804 ; SYNW0289 .
GeneIDi 1732105.
KEGGi syw:SYNW0289.
PATRICi 23831959. VBISynSp27240_0292.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WH8102.

Entry informationi

Entry nameiSYI_SYNPX
AccessioniPrimary (citable) accession number: Q7U9G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2003
Last modified: September 3, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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