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Q7U9G9

- SYI_SYNPX

UniProt

Q7U9G9 - SYI_SYNPX

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Synechococcus sp. (strain WH8102)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Oct 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei585 – 5851Aminoacyl-adenylateUniRule annotation
    Binding sitei629 – 6291ATPUniRule annotation
    Metal bindingi939 – 9391ZincUniRule annotation
    Metal bindingi942 – 9421ZincUniRule annotation
    Metal bindingi959 – 9591ZincUniRule annotation
    Metal bindingi962 – 9621ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:SYNW0289
    OrganismiSynechococcus sp. (strain WH8102)
    Taxonomic identifieri84588 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
    ProteomesiUP000001422: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 974974Isoleucine--tRNA ligasePRO_0000098491Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi84588.SYNW0289.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7U9G9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi69 – 7911"HIGH" regionAdd
    BLAST
    Motifi626 – 6305"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q7U9G9-1 [UniParc]FASTAAdd to Basket

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    MSKETRDAAA EGRPSYKDTL NLLQTGFGMR ANAVKREPEL QAFWSDNGID    50
    GQLGLQNDGP TFTLHDGPPY ANGALHMGHA LNKVLKDVIN KYQVLKGRRV 100
    RYVPGWDCHG LPIELKVLQS MDQEQRKALT PIKLRKKAAA YARKQVDGQM 150
    KGFQRWGIWA DWEQPYLTLQ KEYEAAQIKV FGEMVLKGHI YRGLKPVHWS 200
    PSSRTALAEA ELEYPDGHTS PSVYVAFPAM EVPTPLRDAL KAEGLELPTE 250
    TDALRQALQV AIWTTTPWTL PANLAVSVNE RLDYALVDDG SGRMLVVAAD 300
    LIESLSTTLE RPLKHRATVK GALLAGLIYR HPLLDRTSPV VIGGEYITTE 350
    SGTGLVHTAP GHGVDDFHTG QKHGLPVLCP VDEAGTLTAE AGPFAGLNVL 400
    KDANPGIIEA LEQAGALLKQ EAYSHRYPYD WRTKKPTIFR ATEQWFASVE 450
    GFRQDALDAI DQVQWTPASG RNRIEAMVKE RGDWCISRQR TWGVPIPVFY 500
    HHSNGEVLLN ADTLSHIETL IASHGADVWW EKDETDLLPP AYANQADQWR 550
    KGTDTMDVWF DSGSSWAAVS SQRESLSYPA DLYLEGSDQH RGWFQSSLLT 600
    SVAVNGHAPY KRVLTHGFAL DEKGRKMSKS LGNVVDPMVI IEGGKNQKQE 650
    PPYGADVLRL WVSSVDYSAD VPIGAGILRQ LADVYRKVRN TSRYLLGNLH 700
    DFNPASDAIA VADLPLLDRW MLQRTAEVMH EITEAFESYE FFRFFQLLQN 750
    FCVTDLSNFY LDIAKDRLYV SAPTDQRRRS CQTVMALIIE RLAGFIAPVL 800
    CHMAEDIWQN LPYPVQETSV FQRGWPTIPS DWRNDTLSAP VQQLRDLRAA 850
    VNKVLEDCRG RQELGASLEA AVRIDARSPE LQAALSWLND NGDPDVDGLR 900
    DWLLVSQLQL GGEPWAEVLS NHEDELALIE VSRARGTKCE RCWHYEGDVG 950
    QHPDHAHICG RCVGVLERRT HQLV 974
    Length:974
    Mass (Da):108,998
    Last modified:October 1, 2003 - v1
    Checksum:iDF0058516CAE6565
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX569689 Genomic DNA. Translation: CAE06804.1.
    RefSeqiNP_896384.1. NC_005070.1.
    WP_011127163.1. NC_005070.1.

    Genome annotation databases

    EnsemblBacteriaiCAE06804; CAE06804; SYNW0289.
    GeneIDi1732105.
    KEGGisyw:SYNW0289.
    PATRICi23831959. VBISynSp27240_0292.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX569689 Genomic DNA. Translation: CAE06804.1 .
    RefSeqi NP_896384.1. NC_005070.1.
    WP_011127163.1. NC_005070.1.

    3D structure databases

    ProteinModelPortali Q7U9G9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 84588.SYNW0289.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAE06804 ; CAE06804 ; SYNW0289 .
    GeneIDi 1732105.
    KEGGi syw:SYNW0289.
    PATRICi 23831959. VBISynSp27240_0292.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: WH8102.

    Entry informationi

    Entry nameiSYI_SYNPX
    AccessioniPrimary (citable) accession number: Q7U9G9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: October 1, 2003
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3