Q7U8Z6 (QUEF_SYNPX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	SYNW0463

Organism

Synechococcus sp. (strain WH8102) [Complete proteome] [HAMAP]

Taxonomic identifier

84588 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	133 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00818
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00818
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00818
Subcellular location	Cytoplasm Probable HAMAP MF_00818.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 133	133	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00818		PRO_0000163012

Sequences

	Sequence		Length	Mass (Da)
	Q7U8Z6 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 83F264B2434912EF	FASTA	133	15,167
	10 20 30 40 50 60 MTDQLTQTPL YGERAIAEAE LICFDNPRPG RPYEVSIELP EFTCKCPFSS YPDFAVLRLI 70 80 90 100 110 120 YQPGPRVVEL KAIKLYVNSY RDQSISHEEV TNRILDDLVA ATDPVWMQLE ADFNPRGNVH 130 TVVRVSHGTR QPC

References

[1]

"The genome of a motile marine Synechococcus." Palenik B., Brahamsha B., Larimer F.W., Land M.L., Hauser L., Chain P., Lamerdin J.E., Regala W., Allen E.E., McCarren J., Paulsen I.T., Dufresne A., Partensky F., Webb E.A., Waterbury J. Nature 424:1037-1042(2003) [PubMed: 12917641] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WH8102.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX569690 Genomic DNA. Translation: CAE06978.1.
RefSeq	NP_896558.1. NC_005070.1.
3D structure databases
ProteinModelPortal	Q7U8Z6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7U8Z6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1730026.
GenomeReviews	Gene locus SYNW0463 in contig BX548020_GR.
KEGG	syw:SYNW0463.
PATRIC	23832315. VBISynSp27240_0468.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0780.
HOGENOM	HBG294103.
OMA	FRNAGIF.
PhylomeDB	Q7U8Z6.
ProtClustDB	PRK13258.
Enzyme and pathway databases
BioCyc	SSP84588:SYNW0463OR1440-MONOMER.
Family and domain databases
HAMAP	MF_00818. QueF_type1. [Tree]
InterPro	IPR016856. CN_OxRdtase_NADPH-dep_QueF. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K09457.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMs	TIGR03139. QueF-II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_SYNPX

Accession

Primary (citable) accession number: Q7U8Z6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program