Q7U8M8 (GLUQ_SYNPX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamyl-Q tRNA(Asp) synthetase
Short name=Glu-Q-RSs
EC=6.1.1.-

Gene names

Name:	gluQ
Ordered Locus Names:	SYNW0588

Organism

Synechococcus sp. (strain WH8102) [Complete proteome] [HAMAP]

Taxonomic identifier

84588 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5-dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble position of the QUC anticodon By similarity. HAMAP MF_01428
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_01428
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily.

Ontologies

Keywords
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro tRNA modification Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	Glutamyl-Q tRNA(Asp) synthetase HAMAP MF_01428		PRO_0000208330
Regions
	Region	26 – 30	5	Glutamate binding By similarity
	Motif	29 – 39	11	"HIGH" region HAMAP MF_01428
	Motif	234 – 238	5	"KMSKS" region HAMAP MF_01428
Sites
	Metal binding	118	1	Zinc By similarity
	Metal binding	120	1	Zinc By similarity
	Metal binding	131	1	Zinc By similarity
	Metal binding	135	1	Zinc By similarity
	Binding site	62	1	Glutamate By similarity
	Binding site	178	1	Glutamate By similarity
	Binding site	196	1	Glutamate By similarity
	Binding site	237	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7U8M8 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 8F2EC7CB6DCEC591	FASTA	293	32,388
	10 20 30 40 50 60 MPVPDHLLLE LERGQRWRAD GTYRGRYAPS PTGALHLGNL QTALLSWLQA RQAGGVWLLR 70 80 90 100 110 120 IDDLDTPRNR PGAVEAIQAD LHWLGLDWDG EPILQSCRRG LYASWLSWFR RSGALFPCRC 130 140 150 160 170 180 SRRELAGLAR YPGTCRDGGA GWGWRDRRLP SWRLRVPSRD PDGSGDVVVR RADGFIAYQL 190 200 210 220 230 240 ATVIDELALG ITDVVRGEDL REALPAQRSV YRALQQQPPR FHHGPLRCDA EGRKLSKREA 250 260 270 280 290 SSGLMALREL GLDAPAVVGL LASGLGYGPP GARLSAIELL EDLTQKGIRA GHS

References

[1]

"The genome of a motile marine Synechococcus." Palenik B., Brahamsha B., Larimer F.W., Land M.L., Hauser L., Chain P., Lamerdin J.E., Regala W., Allen E.E., McCarren J., Paulsen I.T., Dufresne A., Partensky F., Webb E.A., Waterbury J. Nature 424:1037-1042(2003) [PubMed: 12917641] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WH8102.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX569690 Genomic DNA. Translation: CAE07103.1.
RefSeq	NP_896681.1. NC_005070.1.
3D structure databases
ProteinModelPortal	Q7U8M8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7U8M8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1731557.
GenomeReviews	Gene locus SYNW0588 in contig BX548020_GR.
KEGG	syw:SYNW0588.
PATRIC	23832581. VBISynSp27240_0600.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	DAPSSYH.
PhylomeDB	Q7U8M8.
ProtClustDB	PRK05710.
Enzyme and pathway databases
BioCyc	SSP84588:SYNW0588OR1522-MONOMER.
Family and domain databases
HAMAP	MF_01428. Glu_Q_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR022380. Glu-Q_TRNA(Asp)_Synthase. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 2 hits. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLUQ_SYNPX

Accession

Primary (citable) accession number: Q7U8M8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program