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Protein

Lipoyl synthase 2

Gene

lipA2

Organism
Synechococcus sp. (strain WH8102)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (lipB)
  2. Lipoyl synthase 1 (lipA1), Lipoyl synthase 2 (lipA2)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi43Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi48Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi54Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi69Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi73Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi76Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase 2UniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-syn 2UniRule annotation
Lipoate synthase 2UniRule annotation
Lipoic acid synthase 2UniRule annotation
Sulfur insertion protein lipA2
Gene namesi
Name:lipA2UniRule annotation
Synonyms:lipA
Ordered Locus Names:SYNW0929
OrganismiSynechococcus sp. (strain WH8102)
Taxonomic identifieri84588 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus
Proteomesi
  • UP000001422 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001023701 – 299Lipoyl synthase 2Add BLAST299

Interactioni

Protein-protein interaction databases

STRINGi84588.SYNW0929

Structurei

3D structure databases

ProteinModelPortaliQ7U7Q2
SMRiQ7U7Q2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0G Bacteria
COG0320 LUCA
HOGENOMiHOG000235997
KOiK03644
OMAiGRCPNRG
OrthoDBiPOG091H069D

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

Q7U7Q2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKYSTIAPT ERLPEWLRRP IGDASALERV QGLVKQNRLH TICEEGRCPN
60 70 80 90 100
RGECYAAGTA TFLLGGSICT RSCAFCQVEK GQAPMAVDPA EAQRVADAVE
110 120 130 140 150
AMQLRYVVLT AVARDDLADH GAALFTTTME AIRARNPLIA IEVLTPDFWG
160 170 180 190 200
GCPDPQQAVA AQRERLSTVL AADPVCFNHN LETVQRLQGE VRRGATYERS
210 220 230 240 250
LGLLAACREL APTIPTKSGL MLGLGESRDE VIAAMRDLRA VDCQRLTLGQ
260 270 280 290
YLRPSLAHIP VDRYWTPEEF DELGAVARDL GFAQVRSGPL VRSSYHAAD
Length:299
Mass (Da):32,691
Last modified:October 1, 2003 - v1
Checksum:i89637339E040CC16
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX569691 Genomic DNA Translation: CAE07444.1
RefSeqiWP_011127794.1, NC_005070.1

Genome annotation databases

EnsemblBacteriaiCAE07444; CAE07444; SYNW0929
KEGGisyw:SYNW0929

Similar proteinsi

Entry informationi

Entry nameiLIPA2_SYNPX
AccessioniPrimary (citable) accession number: Q7U7Q2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: October 1, 2003
Last modified: May 23, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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