Reviewed, UniProtKB/Swiss-Prot Q7U6Y8 (ACSF_SYNPX)

Names and origin

Protein names

Recommended name:
    Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase
      Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase
    EC=1.14.13.81

Gene names

Name:	acsF
Ordered Locus Names:	SYNW1198

Organism

Synechococcus sp. (strain WH8102) [Complete proteome] [HAMAP]

Taxonomic identifier

84588 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity. HAMAP MF_01840
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O. HAMAP MF_01840 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O. HAMAP MF_01840 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = divinylprotochlorophyllide + NADP+ + 2 H2O. HAMAP MF_01840
Cofactor	Iron By similarity. HAMAP MF_01840
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis (light-independent). HAMAP MF_01840
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: HAMAP magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase HAMAP MF_01840		PRO_0000217538

Sequences

	Sequence		Length	Mass (Da)
	Q7U6Y8-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 7A5F8C163703EE0B	FASTA	356	41,413
	10 20 30 40 50 60 MVPPTAVAEA NAVATKDPVK DTILTPRFYT TDFDDMAAMD LRPNEAELEA ICEEFRKDYN 70 80 90 100 110 120 RHHFVRNDDF DGAADKLDPE TRRVFVEFLE QSCTSEFSGF LLYKELSRRI KQQNPLLAEC 130 140 150 160 170 180 FAHMARDEAR HAGFLNKAMS DFGMQLDLGF LTANKDYTFF QPKFIFYATY LSEKIGYWRY 190 200 210 220 230 240 IAIYRHLEKN PESKIFPIFN FFENWCQDEN RHGDFFDALM KSQPDTVRGP IAKLWCRFFL 250 260 270 280 290 300 LAVFATMYVR DVARKEFYEA LGLDARTYDK MVIEKTNETT ARVFPVVLDV NNPKFWTRLE 310 320 330 340 350 RLVENNAALE AADRSSSPAP LKLFKKLPRW ISNGAEMAKL FLMSPIDSAK FQPAVR

References

[1]

"The genome of a motile marine Synechococcus." Palenik B., Brahamsha B., Larimer F.W., Land M.L., Hauser L., Chain P., Lamerdin J.E., Regala W., Allen E.E., McCarren J., Paulsen I.T., Dufresne A., Partensky F., Webb E.A., Waterbury J. Nature 424:1037-1042(2003) [PubMed: 12917641] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX569692 Genomic DNA. Translation: CAE07713.1.
RefSeq	NP_897291.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q7U6Y8.
Genome annotation databases
GeneID	1731553.
KEGG	syw:SYNW1198.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG10633.
HOGENOM	HBG284843.
OMA	YARYITI.
Enzyme and pathway databases
BioCyc	SSP84588:SYNW1198OR3403-MONOMER.
Family and domain databases
HAMAP	MF_01840. AcsF. [Tree]
InterPro	IPR008434. AcsF. IPR009078. Ferritin/RR-like. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACSF_SYNPX

Accession

Primary (citable) accession number: Q7U6Y8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	October 1, 2003
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)