Q7U5G1 (ILVB_SYNPX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetolactate synthase large subunit
Short name=AHAS
EC=2.2.1.6

Alternative name(s):
Acetohydroxy-acid synthase large subunit
Short name=ALS

Gene names

Name:	ilvB
Ordered Locus Names:	SYNW1746

Organism

Synechococcus sp. (strain WH8102) [Complete proteome] [HAMAP]

Taxonomic identifier

84588 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	617 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 pyruvate = 2-acetolactate + CO2.
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.
Subunit structure	Dimer of large and small chains By similarity.
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	FAD Flavoprotein Magnesium Metal-binding Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acetolactate synthase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: InterPro thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 617	617	Acetolactate synthase large subunit		PRO_0000090806
Regions
	Nucleotide binding	281 – 302	22	FAD By similarity
	Nucleotide binding	324 – 343	20	FAD By similarity
	Region	413 – 492	80	Thiamine pyrophosphate binding
Sites
	Metal binding	463	1	Magnesium By similarity
	Metal binding	490	1	Magnesium By similarity
	Binding site	71	1	Thiamine pyrophosphate By similarity
	Binding site	173	1	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7U5G1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 1DC3EF3BB5086E2F	FASTA	617	65,621
	10 20 30 40 50 60 MTLTSASTVV GGLDANAPQT ISGAAALMDA LRRHGVDTIF GYPGGAILPI YDALHIAESE 70 80 90 100 110 120 GWVKHILVRH EQAGTHAADA YARATGKVGV CFGTSGPGAT NLVTGIATAQ MDSVPMVVIT 130 140 150 160 170 180 GQVPRPAIGT DAFQETDIFG ITLPIVKHSW VVRDPADLGS IVAQAFLIAA SGRPGPVLID 190 200 210 220 230 240 IPKDVGQEQF NYVPVEPGSV IPGGFHQPEP PLDAAVAAAL DLIEQAQRPL LYVGGGAISA 250 260 270 280 290 300 CAHDSLRMLA ERYQLPVTTT LMGKGAFDEN DALSVGMLGM HGTAYANFAV TECDLLIAVG 310 320 330 340 350 360 ARFDDRVTGK LDTFAPRARV VHFEIDPAEI GKNRKADVAV LGDLGLSLAR MVEISLQRTA 370 380 390 400 410 420 EPRTAAWLER INTWKDRYPL TIPPAEGAIY PQEVLLAVRD LAPDAIVTTD VGQHQMWAAQ 430 440 450 460 470 480 HLRNGPRGWI SSAGLGTMGF GMPAAMGAQV AMPDRQVVCI AGDASILMNI QELGTLAAYG 490 500 510 520 530 540 LPVKVVIVNN HWQGMVRQWQ ESFYDERYSA SDMLNGMPDF IALARSFGVD GVKITDRELL 550 560 570 580 590 600 HRDLAAALQS PTPTMIDVHV RRGENCYPMV PPGKSNAQMV GLPSHPELAM GTTRTCSSCG 610 AITAHEHRFC PQCGASL

References

[1]

"The genome of a motile marine Synechococcus." Palenik B., Brahamsha B., Larimer F.W., Land M.L., Hauser L., Chain P., Lamerdin J.E., Regala W., Allen E.E., McCarren J., Paulsen I.T., Dufresne A., Partensky F., Webb E.A., Waterbury J. Nature 424:1037-1042(2003) [PubMed: 12917641] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WH8102.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX569693 Genomic DNA. Translation: CAE08261.1.
RefSeq	NP_897837.1. NC_005070.1.
3D structure databases
ProteinModelPortal	Q7U5G1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7U5G1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1731339.
GenomeReviews	Gene locus SYNW1746 in contig BX548020_GR.
KEGG	syw:SYNW1746.
PATRIC	23835167. VBISynSp27240_1878.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0028.
HOGENOM	HBG323037.
OMA	GPRGWIS.
PhylomeDB	Q7U5G1.
ProtClustDB	PRK07418.
Enzyme and pathway databases
BioCyc	SSP84588:SYNW1746OR2980-MONOMER.
Family and domain databases
InterPro	IPR012846. Acetolactate_synth_lsu. IPR012000. Thiamin_PyroP_enz_cen_dom. IPR012001. Thiamin_PyroP_enz_TPP-bd_dom. IPR000399. TPP-bd_CS. IPR011766. TPP_enzyme-bd_C. [Graphical view]
KO	K01652.
PANTHER	PTHR18968:SF13. PTHR18968:SF13. 1 hit.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00118. Acolac_lg. 1 hit.
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVB_SYNPX

Accession

Primary (citable) accession number: Q7U5G1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program