Q7U581 (SYE_SYNPX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	SYNW1826

Organism

Synechococcus sp. (strain WH8102) [Complete proteome] [HAMAP]

Taxonomic identifier

84588 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 477	477	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000119678
Regions
	Motif	8 – 18	11	"HIGH" region HAMAP MF_00022_B
	Motif	247 – 251	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	250	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7U581 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: 152F9000D56D5E29	FASTA	477	53,329
	10 20 30 40 50 60 MVRVRLAPSP TGTLHIGTAR TAVFNWLYAR RQQGSFLLRI EDTDKERSKP EYTQNILEGL 70 80 90 100 110 120 RWLGIDWDEE PLIQSEQVQQ HRAAIETLLQ KGLAYRCYAN EAELDAMREA QKASNQAPRY 130 140 150 160 170 180 DNRHRNLTPE QEAAFQSEGR EAVIRFRIDD NAEIRWNDMV RGAMSWRGAD LGGDMVVARR 190 200 210 220 230 240 APADQIGDPL YNLVVVVDDA AMAISHVIRG EDHIANTAKQ LLLYEALDLP APTFAHAPLI 250 260 270 280 290 300 LNAEGRKLSK RDGVTSINDF RTMGYTAEAI ANYMTLLGWS VPEGMEERFT LPEAAAVFSF 310 320 330 340 350 360 DRVNKAGARF DWDKLNWLNG QVLHALPAQQ LLDDLRPLWA EQGWTLPDDS SWGLELCELL 370 380 390 400 410 420 GPSLTLLKEG VEQATPFFKC PDLEDDGVRQ LEADGARTAV AQLLQILEAE PWDGKDTDRA 430 440 450 460 470 KQLLADAAKG AGVKKGVVMK SLRAALLGRL QGPDLITTWC LLARIGEDLP RLQRCLA

References

[1]

"The genome of a motile marine Synechococcus." Palenik B., Brahamsha B., Larimer F.W., Land M.L., Hauser L., Chain P., Lamerdin J.E., Regala W., Allen E.E., McCarren J., Paulsen I.T., Dufresne A., Partensky F., Webb E.A., Waterbury J. Nature 424:1037-1042(2003) [PubMed: 12917641] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WH8102.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX569694 Genomic DNA. Translation: CAE08341.1.
RefSeq	NP_897917.1. NC_005070.1.
3D structure databases
ProteinModelPortal	Q7U581.
SMR	Q7U581. Positions 2-476.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7U581.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1731239.
GenomeReviews	Gene locus SYNW1826 in contig BX548020_GR.
KEGG	syw:SYNW1826.
PATRIC	23835335. VBISynSp27240_1960.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	DKETAND.
PhylomeDB	Q7U581.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	SSP84588:SYNW1826OR0662-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_SYNPX

Accession

Primary (citable) accession number: Q7U581

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program