Q7U566 (UCRI_SYNPX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome b6-f complex iron-sulfur subunit
EC=1.10.9.1

Alternative name(s):
Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
Short name=ISP
Short name=RISP
Rieske iron-sulfur protein

Gene names

Name:	petC
Ordered Locus Names:	SYNW1841

Organism

Synechococcus sp. (strain WH8102) [Complete proteome] [HAMAP]

Taxonomic identifier

84588 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechococcus

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions By similarity. HAMAP-Rule MF_01335
Catalytic activity	Plastoquinol + 2 oxidized plastocyanin + 2 H+(Side 1) = plastoquinone + 2 reduced plastocyanin + 2 H+(Side 2). HAMAP-Rule MF_01335
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer By similarity.
Subcellular location	Cellular thylakoid membrane; Single-pass membrane protein By similarity. Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer By similarity. HAMAP-Rule MF_01335
Miscellaneous	The Rieske iron-sulfur protein is a high potential 2Fe-2S protein. HAMAP-Rule MF_01335
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Membrane Thylakoid
Domain	Transmembrane Transmembrane helix
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW plastoquinol--plastocyanin reductase activity Inferred from electronic annotation. Source: HAMAP ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 178	178	Cytochrome b6-f complex iron-sulfur subunit HAMAP-Rule MF_01335		PRO_0000127783
Regions
	Transmembrane	20 – 42	23	Helical; Potential
	Domain	65 – 161	97	Rieske
Sites
	Metal binding	107	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	109	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	125	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	128	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	112 ↔ 127		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7U566 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: DE3A1443271528A4	FASTA	178	18,735
	10 20 30 40 50 60 MTQLSSSDVP GMGRRQFMNL LTFGSVTGVA LGALYPVVNY FIPPRAAGAG GGTTAKDELG 70 80 90 100 110 120 NAITATGWLS SHPEGDRSLV QGLKGDPTYL IVEGPDAIGS YGINAICTHL GCVVPWNSGA 130 140 150 160 170 NKFMCPCHGS QYDATGKVVR GPAPLSLALA NVSVENDNVF VSQWTETDFR TGDKPWWA

References

[1]

"The genome of a motile marine Synechococcus." Palenik B., Brahamsha B., Larimer F.W., Land M.L., Hauser L., Chain P., Lamerdin J.E., Regala W., Allen E.E., McCarren J., Paulsen I.T., Dufresne A., Partensky F., Webb E.A., Waterbury J. Nature 424:1037-1042(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WH8102.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX569694 Genomic DNA. Translation: CAE08356.1.
RefSeq	NP_897932.1. NC_005070.1.
3D structure databases
ProteinModelPortal	Q7U566.
SMR	Q7U566. Positions 8-177.
ModBase	Search...
Protein-protein interaction databases
STRING	84588.SYNW1841.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAE08356; CAE08356; SYNW1841.
GeneID	1730606.
KEGG	syw:SYNW1841.
PATRIC	23835365. VBISynSp27240_1975.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0723.
HOGENOM	HOG000255192.
KO	K02636.
OMA	GDPTYIV.
ProtClustDB	PRK13474.
Family and domain databases
Gene3D	2.102.10.10. 1 hit.
HAMAP	MF_01335. Cytb6_f_Rieske.
InterPro	IPR014909. Cyt_b6-f_cplx_Fe-S_su. IPR023960. Cyt_b6_f_Rieske. IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR005805. Rieske_Fe-S_prot_C. IPR006311. TAT_signal. [Graphical view]
PANTHER	PTHR10134. PTHR10134. 1 hit.
Pfam	PF08802. CytB6-F_Fe-S. 1 hit. PF00355. Rieske. 1 hit. [Graphical view]
PRINTS	PR00162. RIESKE.
SUPFAM	SSF50022. Rieske_dom. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI_SYNPX

Accession

Primary (citable) accession number: Q7U566

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	October 1, 2003
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program