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Protein

Dimethylglycine N-methyltransferase

Gene

bsmB

Organism
Synechococcus sp. (strain WH8102)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methylation of dimethylglycine to betaine with S-adenosylmethionine (AdoMet) acting as the methyl donor. It has strict specificity for dimethylglycine as the methyl group acceptors.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + N,N-dimethylglycine = S-adenosyl-L-homocysteine + betaine.1 Publication

Kineticsi

  1. KM=2.11 mM for dimethylglycine (at pH 8.5 and at 37 degrees Celsius)1 Publication
  2. KM=0.41 mM for AdoMet (at pH 8.5 and at 37 degrees Celsius)1 Publication
  1. Vmax=1.06 µmol/min/mg enzyme with AdoMet as substrate (at pH 8.5 and at 37 degrees Celsius)1 Publication
  2. Vmax=1.18 µmol/min/mg enzyme with dimethylglycine as substrate (at pH 8.5 and at 37 degrees)1 Publication

pH dependencei

Optimum pH is around 8.5 for dimethylglycine.1 Publication

Pathwayi: betaine biosynthesis via glycine pathway

This protein is involved in step 3 of the subpathway that synthesizes betaine from glycine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycine/sarcosine N-methyltransferase (bsmA)
  2. Glycine/sarcosine N-methyltransferase (bsmA)
  3. Dimethylglycine N-methyltransferase (bsmB)
This subpathway is part of the pathway betaine biosynthesis via glycine pathway, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes betaine from glycine, the pathway betaine biosynthesis via glycine pathway and in Amine and polyamine biosynthesis.

GO - Molecular functioni

  • dimethylglycine N-methyltransferase activity Source: UniProtKB

GO - Biological processi

  • glycine betaine biosynthetic process from glycine Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00530; UER00383.

Names & Taxonomyi

Protein namesi
Recommended name:
Dimethylglycine N-methyltransferase (EC:2.1.1.161)
Gene namesi
Name:bsmB
Synonyms:sdmt
Ordered Locus Names:SYNW1913
OrganismiSynechococcus sp. (strain WH8102)
Taxonomic identifieri84588 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
Proteomesi
  • UP000001422 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 280280Dimethylglycine N-methyltransferasePRO_0000413615Add
BLAST

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi84588.SYNW1913.

Structurei

3D structure databases

ProteinModelPortaliQ7U4Z9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the methyltransferase superfamily.Curated

Phylogenomic databases

eggNOGiENOG4107SK7. Bacteria.
COG0500. LUCA.
HOGENOMiHOG000230375.
KOiK13042.
OMAiTIWGGED.
OrthoDBiEOG6W45XP.

Family and domain databases

Gene3Di1.10.287.840. 2 hits.
3.40.50.150. 1 hit.
InterProiIPR023143. Cyclopro_synthase-like_dom.
IPR013216. Methyltransf_11.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7U4Z9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTTNGCAAD SVAATYYDSQ DADQFYEQVW GGEDIHIGLY ATPDEAIATA
60 70 80 90 100
SDRTVHALLD LADPLPQGGC VVDLGAGYGG ASRRLARWSE RPVHAINISA
110 120 130 140 150
VENDRHRRLN VDAGLEQQIT VHDASFEQVP MADASADLVW SQDAILHAGD
160 170 180 190 200
RAKVLAEVSR LLKPGGCFVF TDPMAADGVE MGLLQPILDR IHLPDLASPS
210 220 230 240 250
RYKAWGEAVG LTMEVWDERT EMLVRHYDRV RQDTRLRRAE LETSISSGYL
260 270 280
DRMDVGLGHW VDGGQQGRLS WGLMRLRKPG
Length:280
Mass (Da):30,752
Last modified:October 1, 2003 - v1
Checksum:i711CFCD662447E74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX569694 Genomic DNA. Translation: CAE08428.1.
RefSeqiWP_011128771.1. NC_005070.1.

Genome annotation databases

EnsemblBacteriaiCAE08428; CAE08428; SYNW1913.
KEGGisyw:SYNW1913.
PATRICi23835513. VBISynSp27240_2047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX569694 Genomic DNA. Translation: CAE08428.1.
RefSeqiWP_011128771.1. NC_005070.1.

3D structure databases

ProteinModelPortaliQ7U4Z9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi84588.SYNW1913.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE08428; CAE08428; SYNW1913.
KEGGisyw:SYNW1913.
PATRICi23835513. VBISynSp27240_2047.

Phylogenomic databases

eggNOGiENOG4107SK7. Bacteria.
COG0500. LUCA.
HOGENOMiHOG000230375.
KOiK13042.
OMAiTIWGGED.
OrthoDBiEOG6W45XP.

Enzyme and pathway databases

UniPathwayiUPA00530; UER00383.

Family and domain databases

Gene3Di1.10.287.840. 2 hits.
3.40.50.150. 1 hit.
InterProiIPR023143. Cyclopro_synthase-like_dom.
IPR013216. Methyltransf_11.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WH8102.
  2. "Identification of glycine betaine as compatible solute in Synechococcus sp. WH8102 and characterization of its N-methyltransferase genes involved in betaine synthesis."
    Lu W.D., Chi Z.M., Su C.D.
    Arch. Microbiol. 186:495-506(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN BETAINE BIOSYNTHESIS, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, NOMENCLATURE.

Entry informationi

Entry nameiSDMT_SYNPX
AccessioniPrimary (citable) accession number: Q7U4Z9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: October 1, 2003
Last modified: November 11, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.