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Protein

Glycine/sarcosine N-methyltransferase

Gene

bsmA

Organism
Synechococcus sp. (strain WH8102)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methylation of glycine and sarcosine to sarcosine and dimethylglycine, respectively, with S-adenosylmethionine (AdoMet) acting as the methyl donor. It has strict specificity for glycine and sarcosine as the methyl group acceptors.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine.1 Publication
2 S-adenosyl-L-methionine + glycine = 2 S-adenosyl-L-homocysteine + N,N-dimethylglycine.1 Publication

Kineticsi

  1. KM=0.30 mM for AdoMet (with glycine at pH 8.2 and at 37 degrees Celsius)1 Publication
  2. KM=0.44 mM for AdoMet (with sarcosine at pH 8.0 and at 37 degrees Celsius)1 Publication
  3. KM=2.94 mM for sarcosine (at pH 8.0 and at 37 degrees Celsius)1 Publication
  4. KM=5.22 mM for glycine (at pH 8.2 and at 37 degrees Celsius)1 Publication
  1. Vmax=0.74 µmol/min/mg enzyme with AdoMet as substrate (with sarcosine at pH 8.0 and at 37 degrees Celsius)1 Publication
  2. Vmax=0.78 µmol/min/mg enzyme with sarcosine as substrate (at pH 8.0 and at 37 degrees Celsius)1 Publication
  3. Vmax=1.30 µmol/min/mg enzyme with AdoMet as substrate (with glycine at pH 8.2 and at 37 degrees Celsius)1 Publication
  4. Vmax=1.48 µmol/min/mg enzyme with glycine as substrate (at pH 8.2 and at 37 degrees Celsius)1 Publication

pH dependencei

Optimum pH is around 8.2 and 8.0 for glycine and sarcosine, respectively.1 Publication

Pathwayi: betaine biosynthesis via glycine pathway

This protein is involved in step 1 and 2 of the subpathway that synthesizes betaine from glycine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycine/sarcosine N-methyltransferase (bsmA)
  2. Glycine/sarcosine N-methyltransferase (bsmA)
  3. Dimethylglycine N-methyltransferase (bsmB)
This subpathway is part of the pathway betaine biosynthesis via glycine pathway, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes betaine from glycine, the pathway betaine biosynthesis via glycine pathway and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei43 – 431S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei52 – 521S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei76 – 761S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei97 – 971S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei141 – 1411S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation
Binding sitei143 – 1431SubstratePROSITE-ProRule annotation
Binding sitei176 – 1761SubstratePROSITE-ProRule annotation
Binding sitei217 – 2171SubstratePROSITE-ProRule annotation

GO - Molecular functioni

  • glycine N-methyltransferase activity Source: UniProtKB
  • sarcosine N-methyltransferase activity Source: UniProtKB

GO - Biological processi

  • glycine betaine biosynthetic process from glycine Source: UniProtKB
  • methylation Source: UniProtKB

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.156. 9130.
UniPathwayiUPA00530; UER00381.
UPA00530; UER00382.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine/sarcosine N-methyltransferase (EC:2.1.1.156)
Gene namesi
Name:bsmA
Synonyms:gsmt
Ordered Locus Names:SYNW1914
OrganismiSynechococcus sp. (strain WH8102)
Taxonomic identifieri84588 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
Proteomesi
  • UP000001422 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 282282Glycine/sarcosine N-methyltransferasePRO_0000413612Add
BLAST

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi84588.SYNW1914.

Structurei

3D structure databases

ProteinModelPortaliQ7U4Z8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni123 – 1242S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4107RAX. Bacteria.
COG0500. LUCA.
HOGENOMiHOG000230307.
KOiK18896.
OMAiDHYQQEY.
OrthoDBiPOG091H03FK.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR014369. Gly/Sar_N_MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR16458. PTHR16458. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51600. SAM_GNMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7U4Z8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSTQNHPLQ TQDDQQRFGQ SPESVRETDH YQQEYIEDFT DRWDRLIDWN
60 70 80 90 100
ARAEAEGDFF IRLLKEHGAR SVLDVATGTG FHSIRLLEEG FDVVSADGSP
110 120 130 140 150
NMLARAFRNA RNRDQLLRTS QADWRFLNRD IHGEFDAVIC LGNSFTHLFK
160 170 180 190 200
ERDRRKALAE YYAVLKHNGI LILDHRNYDR LLEGGSAVRQ GKGNVYCGKD
210 220 230 240 250
VEVGPEHVDE GLARFRYSFS DGGVYHLNMF PLRYGYVRRL MSEVGFQQIT
260 270 280
SFGDYQRDFE NPDFYVHVAE KEYRFDVDTT MH
Length:282
Mass (Da):32,894
Last modified:October 1, 2003 - v1
Checksum:i0A3235C5445874DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX569694 Genomic DNA. Translation: CAE08429.1.
RefSeqiWP_011128772.1. NC_005070.1.

Genome annotation databases

EnsemblBacteriaiCAE08429; CAE08429; SYNW1914.
KEGGisyw:SYNW1914.
PATRICi23835515. VBISynSp27240_2048.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX569694 Genomic DNA. Translation: CAE08429.1.
RefSeqiWP_011128772.1. NC_005070.1.

3D structure databases

ProteinModelPortaliQ7U4Z8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi84588.SYNW1914.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE08429; CAE08429; SYNW1914.
KEGGisyw:SYNW1914.
PATRICi23835515. VBISynSp27240_2048.

Phylogenomic databases

eggNOGiENOG4107RAX. Bacteria.
COG0500. LUCA.
HOGENOMiHOG000230307.
KOiK18896.
OMAiDHYQQEY.
OrthoDBiPOG091H03FK.

Enzyme and pathway databases

UniPathwayiUPA00530; UER00381.
UPA00530; UER00382.
BRENDAi2.1.1.156. 9130.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR014369. Gly/Sar_N_MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR16458. PTHR16458. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51600. SAM_GNMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSMT_SYNPX
AccessioniPrimary (citable) accession number: Q7U4Z8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: October 1, 2003
Last modified: September 7, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.