Q7U4U9 (MTNC_SYNPX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Enolase-phosphatase E1
EC=3.1.3.77

Alternative name(s):
2,3-diketo-5-methylthio-1-phosphopentane phosphatase

Gene names

Name:	mtnC
Ordered Locus Names:	SYNW1964

Organism

Synechococcus sp. (strain WH8102) [Complete proteome] [HAMAP]

Taxonomic identifier

84588 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) By similarity. HAMAP MF_01681
Catalytic activity	5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate. HAMAP MF_01681
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01681
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. HAMAP MF_01681 Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.
Subunit structure	Monomer By similarity. HAMAP MF_01681
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. MasA/mtnC family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-methionine salvage from methylthioadenosine Inferred from electronic annotation. Source: InterPro
Molecular function	2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity Inferred from electronic annotation. Source: InterPro 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity Inferred from electronic annotation. Source: InterPro acireductone synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Enolase-phosphatase E1 HAMAP MF_01681		PRO_0000357425

Sequences

	Sequence		Length	Mass (Da)
	Q7U4U9 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: ECF6D582E9251430	FASTA	245	28,136
	10 20 30 40 50 60 MSITHLLIDI EGTTCPVSFV SEVLFPYAKQ SLSSYLNDHQ EDLDLKNILQ EAEREWDGDP 70 80 90 100 110 120 SPDSIKLRQA TRNQNLNFID SIKVYFEYLI NVDRKSTALK DLQGKIWDNG YRKGEITSHL 130 140 150 160 170 180 FNETTECLKR WHRRQLSLSV YSSGSIQAQK LLYRHTNDGD LEHLFDHWFD THTGNKKECA 190 200 210 220 230 240 SYRKIATKIS TNPSEILFIS DNGEECDAAG ASGMETLFSL RDGNPDQSPR SHRVIKTLND VDEYL

References

[1]

"The genome of a motile marine Synechococcus." Palenik B., Brahamsha B., Larimer F.W., Land M.L., Hauser L., Chain P., Lamerdin J.E., Regala W., Allen E.E., McCarren J., Paulsen I.T., Dufresne A., Partensky F., Webb E.A., Waterbury J. Nature 424:1037-1042(2003) [PubMed: 12917641] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WH8102.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX569694 Genomic DNA. Translation: CAE08479.1.
RefSeq	NP_898055.1. NC_005070.1.
3D structure databases
HSSP	HSSP built from PDB template 2G80 based on UniProtKB P32626.
ProteinModelPortal	Q7U4U9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7U4U9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1730515.
GenomeReviews	Gene locus SYNW1964 in contig BX548020_GR.
KEGG	syw:SYNW1964.
PATRIC	23835621. VBISynSp27240_2099.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4229.
HOGENOM	HBG294242.
OMA	TTDLNFI.
PhylomeDB	Q7U4U9.
ProtClustDB	CLSK866737.
Enzyme and pathway databases
BioCyc	SSP84588:SYNW1964OR2695-MONOMER.
Family and domain databases
HAMAP	MF_01681. Salvage_MtnC. [Tree]
InterPro	IPR023943. Enolase-phosphatase_E1. IPR010041. Enolase_ppase. IPR023214. HAD-like_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.1000. HAD-like_dom. 1 hit.
KO	K09880.
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01691. Enolase-ppase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTNC_SYNPX

Accession

Primary (citable) accession number: Q7U4U9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program