Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lipoyl synthase 1

Gene

lipA1

Organism
Synechococcus sp. (strain WH8102)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N6-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (lipB)
  2. Lipoyl synthase 1 (lipA1), Lipoyl synthase 2 (lipA2)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi33Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi38Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi44Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi59Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi63Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi66Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase 1UniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-syn 1UniRule annotation
Lipoate synthase 1UniRule annotation
Lipoic acid synthase 1UniRule annotation
Sulfur insertion protein lipA1
Gene namesi
Name:lipA1UniRule annotation
Synonyms:lipA
Ordered Locus Names:SYNW2130
OrganismiSynechococcus sp. (strain WH8102)
Taxonomic identifieri84588 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus
Proteomesi
  • UP000001422 Componenti: Chromosome

Subcellular locationi

Q7U4D9:
  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001023691 – 289Lipoyl synthase 1Add BLAST289

Interactioni

Protein-protein interaction databases

STRINGi84588.SYNW2130.

Structurei

3D structure databases

ProteinModelPortaliQ7U4D9.
SMRiQ7U4D9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0G. Bacteria.
COG0320. LUCA.
HOGENOMiHOG000235997.
KOiK03644.
OMAiPYCDIDF.
OrthoDBiPOG091H069D.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_00206. Lipoyl_synth. 1 hit.
InterProiView protein in InterPro
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
PfamiView protein in Pfam
PF04055. Radical_SAM. 1 hit.
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SFLDiSFLDG01058. lipoyl_synthase_like. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
SMARTiView protein in SMART
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7U4D9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKPEWLRVK APQRERIGAV ADLLLDLNLN TVCQEASCPN IGECFAGGTA
60 70 80 90 100
TFLIMGPGCT RACPYCDIDF DKSVRELDPT EPERLGEAVA RLGLKHVVIT
110 120 130 140 150
SVNRDDLPDG GATQFVACIE QVKQRSPLTT IELLIPDFCG NWDALATVMA
160 170 180 190 200
AAPHVLNHNI ETVPRMYRLA RPQGIYERSL ELLQRVRDDW PRAYSKSGLM
210 220 230 240 250
VGLGETDDEV IDVLRDLRTH RVDIVTIGQY LSPGPKHLAV DRFVTPEQFD
260 270 280
TYRRIGEEEL GFLQVVSTPL TRSSYHAGEV QRLMASHPR
Length:289
Mass (Da):32,291
Last modified:October 1, 2003 - v1
Checksum:iB9F35FA576A99D8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX569694 Genomic DNA. Translation: CAE08645.1.
RefSeqiWP_011128986.1. NC_005070.1.

Genome annotation databases

EnsemblBacteriaiCAE08645; CAE08645; SYNW2130.
KEGGisyw:SYNW2130.

Similar proteinsi

Entry informationi

Entry nameiLIPA1_SYNPX
AccessioniPrimary (citable) accession number: Q7U4D9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: October 1, 2003
Last modified: October 25, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families