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Protein

Lipoyl synthase 1

Gene

lipA1

Organism
Synechococcus sp. (strain WH8102)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (lipB)
  2. Lipoyl synthase 1 (lipA1), Lipoyl synthase 2 (lipA2)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi33Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi38Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi44Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi59Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi63Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi66Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase 1UniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-syn 1UniRule annotation
Lipoate synthase 1UniRule annotation
Lipoic acid synthase 1UniRule annotation
Sulfur insertion protein lipA1
Gene namesi
Name:lipA1UniRule annotation
Synonyms:lipA
Ordered Locus Names:SYNW2130
OrganismiSynechococcus sp. (strain WH8102)
Taxonomic identifieri84588 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus
Proteomesi
  • UP000001422 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001023691 – 289Lipoyl synthase 1Add BLAST289

Interactioni

Protein-protein interaction databases

STRINGi84588.SYNW2130

Structurei

3D structure databases

ProteinModelPortaliQ7U4D9
SMRiQ7U4D9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0G Bacteria
COG0320 LUCA
HOGENOMiHOG000235997
KOiK03644
OMAiPYCDIDF
OrthoDBiPOG091H069D

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

Q7U4D9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKPEWLRVK APQRERIGAV ADLLLDLNLN TVCQEASCPN IGECFAGGTA
60 70 80 90 100
TFLIMGPGCT RACPYCDIDF DKSVRELDPT EPERLGEAVA RLGLKHVVIT
110 120 130 140 150
SVNRDDLPDG GATQFVACIE QVKQRSPLTT IELLIPDFCG NWDALATVMA
160 170 180 190 200
AAPHVLNHNI ETVPRMYRLA RPQGIYERSL ELLQRVRDDW PRAYSKSGLM
210 220 230 240 250
VGLGETDDEV IDVLRDLRTH RVDIVTIGQY LSPGPKHLAV DRFVTPEQFD
260 270 280
TYRRIGEEEL GFLQVVSTPL TRSSYHAGEV QRLMASHPR
Length:289
Mass (Da):32,291
Last modified:October 1, 2003 - v1
Checksum:iB9F35FA576A99D8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX569694 Genomic DNA Translation: CAE08645.1
RefSeqiWP_011128986.1, NC_005070.1

Genome annotation databases

EnsemblBacteriaiCAE08645; CAE08645; SYNW2130
KEGGisyw:SYNW2130

Similar proteinsi

Entry informationi

Entry nameiLIPA1_SYNPX
AccessioniPrimary (citable) accession number: Q7U4D9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: October 1, 2003
Last modified: May 23, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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