Q7U3W8 (GSHB_SYNPX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione synthetase
EC=6.3.2.3

Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase

Gene names

Name:	gshB
Ordered Locus Names:	SYNW2309

Organism

Synechococcus sp. (strain WH8102) [Complete proteome] [HAMAP]

Taxonomic identifier

84588 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP MF_00162
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP MF_00162
Sequence similarities	Belongs to the prokaryotic GSH synthase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutathione synthase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Glutathione synthetase HAMAP MF_00162		PRO_0000197489
Regions
	Domain	120 – 304	185	ATP-grasp
	Nucleotide binding	146 – 202	57	ATP By similarity
Sites
	Metal binding	275	1	Magnesium or manganese By similarity
	Metal binding	277	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7U3W8 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: B67978A17E6FFFB5	FASTA	307	33,471
	10 20 30 40 50 60 MRQLFVLDPL SQIRPEKDST AALMQAAQRA GDDIWSCTPS DLIARGDEPM AVALPVTPDP 70 80 90 100 110 120 WIAVGAPERQ SLAGFDVIWM RKDPPVDEAY LYATHLLEVA ERAGVRVLNR PSALRAWNEK 130 140 150 160 170 180 LGALRFSRWM APTLVAGRVS ELMAFAREQG DVVLKPLGGR AGLGVIRVQA EAPGLKALLE 190 200 210 220 230 240 LVTEQERLPV MAQRFLPDVT EGDKRILLVD GDPLGAVNRR PSEGEFRSNL AVGGQAEATE 250 260 270 280 290 300 LSEPERQICA ALAPALRAEG LFFVGIDVIG GMLSEINVTS PTGVREVERL MQEPLADQTI ERLRSLV

References

[1]

"The genome of a motile marine Synechococcus." Palenik B., Brahamsha B., Larimer F.W., Land M.L., Hauser L., Chain P., Lamerdin J.E., Regala W., Allen E.E., McCarren J., Paulsen I.T., Dufresne A., Partensky F., Webb E.A., Waterbury J. Nature 424:1037-1042(2003) [PubMed: 12917641] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WH8102.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX569695 Genomic DNA. Translation: CAE08824.1.
RefSeq	NP_898398.1. NC_005070.1.
3D structure databases
ProteinModelPortal	Q7U3W8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7U3W8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1730296.
GenomeReviews	Gene locus SYNW2309 in contig BX548020_GR.
KEGG	syw:SYNW2309.
PATRIC	23836372. VBISynSp27240_2456.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0189.
HOGENOM	HBG624526.
OMA	HLTEINV.
PhylomeDB	Q7U3W8.
ProtClustDB	PRK05246.
Enzyme and pathway databases
BioCyc	SSP84588:SYNW2309OR2553-MONOMER.
Family and domain databases
HAMAP	MF_00162. GSH-S. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR006284. Glut_synth_pro. IPR004218. GSHS_ATP-bd. IPR004215. GSHS_N. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KO	K01920.
Pfam	PF02955. GSH-S_ATP. 1 hit. PF02951. GSH-S_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01380. Glut_syn. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHB_SYNPX

Accession

Primary (citable) accession number: Q7U3W8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	October 1, 2003
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program